The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster
Interferon (IFN) system is considered as the first defense line against viral infection, and it has been extensively studied in vertebrates from fish to mammals. In invertebrates, Vagos from arthropod and IFN-like protein ( Cg IFNLP) from Crassostrea gigas appeared to function as IFN-like antiviral...
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crfrontiers:10.3389/fimmu.2021.689783 2024-09-15T18:03:16+00:00 The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster Qiao, Xue Zong, Yanan Liu, Zhaoqun Wu, Zhaojun Li, Yuanmei Wang, Lingling Song, Linsheng 2021 http://dx.doi.org/10.3389/fimmu.2021.689783 https://www.frontiersin.org/articles/10.3389/fimmu.2021.689783/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Immunology volume 12 ISSN 1664-3224 journal-article 2021 crfrontiers https://doi.org/10.3389/fimmu.2021.689783 2024-09-03T04:03:55Z Interferon (IFN) system is considered as the first defense line against viral infection, and it has been extensively studied in vertebrates from fish to mammals. In invertebrates, Vagos from arthropod and IFN-like protein ( Cg IFNLP) from Crassostrea gigas appeared to function as IFN-like antiviral cytokines. In the present study, the Cg IFNLP protein in hemocytes was observed to increase after Poly (I:C) stimulation. After Cg IFNLP was knocked down by RNAi, the mRNA expression of IFN-stimulated genes ( Cg ISGs) was significantly inhibited. Both cyclic GMP-AMP synthase ( Cg cGAS) and stimulator of interferon gene ( Cg STING) identified from oyster were able to recognize the double-stranded nucleic acid [Poly (I:C) and dsDNA] and expressed at high level after Poly (I:C) stimulation. The expression of Cg IFNLP and interferon regulatory factors ( Cg IRF1/8) and the nuclear translocation of Cg IRF8 were all suppressed in Cg cGAS-RNAi or Cg STING-RNAi oysters after Poly (I:C) stimulation. The expression level of Cg STING and TANK binding kinase1 ( Cg TBK1) did not decrease in Cg cGAS-RNAi oysters. After Cg STING was knocked down, the high expression of Cg TBK1 induced by Poly (I:C) was prevented significantly. These results indicated that there was a primitive IFN-like antiviral mechanism dependent on the cGAS/STING–TBK1–IRFs regulatory axis in mollusks, which was different from the classic cGAS–STING–TBK1 signal pathway in mammals. Article in Journal/Newspaper Crassostrea gigas Frontiers (Publisher) Frontiers in Immunology 12 |
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Interferon (IFN) system is considered as the first defense line against viral infection, and it has been extensively studied in vertebrates from fish to mammals. In invertebrates, Vagos from arthropod and IFN-like protein ( Cg IFNLP) from Crassostrea gigas appeared to function as IFN-like antiviral cytokines. In the present study, the Cg IFNLP protein in hemocytes was observed to increase after Poly (I:C) stimulation. After Cg IFNLP was knocked down by RNAi, the mRNA expression of IFN-stimulated genes ( Cg ISGs) was significantly inhibited. Both cyclic GMP-AMP synthase ( Cg cGAS) and stimulator of interferon gene ( Cg STING) identified from oyster were able to recognize the double-stranded nucleic acid [Poly (I:C) and dsDNA] and expressed at high level after Poly (I:C) stimulation. The expression of Cg IFNLP and interferon regulatory factors ( Cg IRF1/8) and the nuclear translocation of Cg IRF8 were all suppressed in Cg cGAS-RNAi or Cg STING-RNAi oysters after Poly (I:C) stimulation. The expression level of Cg STING and TANK binding kinase1 ( Cg TBK1) did not decrease in Cg cGAS-RNAi oysters. After Cg STING was knocked down, the high expression of Cg TBK1 induced by Poly (I:C) was prevented significantly. These results indicated that there was a primitive IFN-like antiviral mechanism dependent on the cGAS/STING–TBK1–IRFs regulatory axis in mollusks, which was different from the classic cGAS–STING–TBK1 signal pathway in mammals. |
format |
Article in Journal/Newspaper |
author |
Qiao, Xue Zong, Yanan Liu, Zhaoqun Wu, Zhaojun Li, Yuanmei Wang, Lingling Song, Linsheng |
spellingShingle |
Qiao, Xue Zong, Yanan Liu, Zhaoqun Wu, Zhaojun Li, Yuanmei Wang, Lingling Song, Linsheng The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster |
author_facet |
Qiao, Xue Zong, Yanan Liu, Zhaoqun Wu, Zhaojun Li, Yuanmei Wang, Lingling Song, Linsheng |
author_sort |
Qiao, Xue |
title |
The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster |
title_short |
The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster |
title_full |
The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster |
title_fullStr |
The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster |
title_full_unstemmed |
The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster |
title_sort |
cgas/sting–tbk1–irf regulatory axis orchestrates a primitive interferon-like antiviral mechanism in oyster |
publisher |
Frontiers Media SA |
publishDate |
2021 |
url |
http://dx.doi.org/10.3389/fimmu.2021.689783 https://www.frontiersin.org/articles/10.3389/fimmu.2021.689783/full |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Frontiers in Immunology volume 12 ISSN 1664-3224 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fimmu.2021.689783 |
container_title |
Frontiers in Immunology |
container_volume |
12 |
_version_ |
1810440775019790336 |