Protein-Based Vaccine Protect Against Piscirickettsia salmonis in Atlantic Salmon (Salmo salar)
An effective and economical vaccine against the Piscirickettsia salmonis pathogen is needed for sustainable salmon farming and to reduce disease-related economic losses. Consequently, the aquaculture industry urgently needs to investigate efficient prophylactic measures. Three protein-based vaccine...
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Online Access: | http://dx.doi.org/10.3389/fimmu.2021.602689 https://www.frontiersin.org/articles/10.3389/fimmu.2021.602689/full |
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crfrontiers:10.3389/fimmu.2021.602689 2024-10-06T13:47:18+00:00 Protein-Based Vaccine Protect Against Piscirickettsia salmonis in Atlantic Salmon (Salmo salar) Pontigo, Juan Pablo Espinoza, Carla Hernandez, Mauricio Nourdin, Guillermo Oliver, Cristian Avendaño-Herrera, Rubén Figueroa, Jaime Rauch, Cecilia Troncoso, José M. Vargas-Chacoff, Luis Yá�ez, Alejandro J. 2021 http://dx.doi.org/10.3389/fimmu.2021.602689 https://www.frontiersin.org/articles/10.3389/fimmu.2021.602689/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Immunology volume 12 ISSN 1664-3224 journal-article 2021 crfrontiers https://doi.org/10.3389/fimmu.2021.602689 2024-09-10T04:03:42Z An effective and economical vaccine against the Piscirickettsia salmonis pathogen is needed for sustainable salmon farming and to reduce disease-related economic losses. Consequently, the aquaculture industry urgently needs to investigate efficient prophylactic measures. Three protein-based vaccine prototypes against Piscirickettsia salmonis were prepared from a highly pathogenic Chilean isolate. Only one vaccine effectively protected Atlantic salmon ( Salmo salar ), in correlation with the induction of Piscirickettsia-specific IgM antibodies and a high induction of transcripts encoding pro-inflammatory cytokines (i.e., Il-1β and TNF-α). In addition, we studied the proteome fraction protein of P. salmonis strain Austral-005 using multidimensional protein identification technology. The analyzes identified 87 proteins of different subcellular origins, such as the cytoplasmic and membrane compartment, where many of them have virulence functions. The other two prototypes activated only the innate immune responses, but did not protect Salmo salar against P. salmonis . These results suggest that the knowledge of the formulation of vaccines based on P. salmonis proteins is useful as an effective therapy, this demonstrates the importance of the different research tools to improve the study of the different immune responses, resistance to diseases in the Atlantic salmon. We suggest that this vaccine can help prevent widespread infection by P. salmonis , in addition to being able to be used as a booster after a primary vaccine to maintain high levels of circulating protective antibodies, greatly helping to reduce the economic losses caused by the pathogen. Article in Journal/Newspaper Atlantic salmon Salmo salar Frontiers (Publisher) Austral Frontiers in Immunology 12 |
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An effective and economical vaccine against the Piscirickettsia salmonis pathogen is needed for sustainable salmon farming and to reduce disease-related economic losses. Consequently, the aquaculture industry urgently needs to investigate efficient prophylactic measures. Three protein-based vaccine prototypes against Piscirickettsia salmonis were prepared from a highly pathogenic Chilean isolate. Only one vaccine effectively protected Atlantic salmon ( Salmo salar ), in correlation with the induction of Piscirickettsia-specific IgM antibodies and a high induction of transcripts encoding pro-inflammatory cytokines (i.e., Il-1β and TNF-α). In addition, we studied the proteome fraction protein of P. salmonis strain Austral-005 using multidimensional protein identification technology. The analyzes identified 87 proteins of different subcellular origins, such as the cytoplasmic and membrane compartment, where many of them have virulence functions. The other two prototypes activated only the innate immune responses, but did not protect Salmo salar against P. salmonis . These results suggest that the knowledge of the formulation of vaccines based on P. salmonis proteins is useful as an effective therapy, this demonstrates the importance of the different research tools to improve the study of the different immune responses, resistance to diseases in the Atlantic salmon. We suggest that this vaccine can help prevent widespread infection by P. salmonis , in addition to being able to be used as a booster after a primary vaccine to maintain high levels of circulating protective antibodies, greatly helping to reduce the economic losses caused by the pathogen. |
format |
Article in Journal/Newspaper |
author |
Pontigo, Juan Pablo Espinoza, Carla Hernandez, Mauricio Nourdin, Guillermo Oliver, Cristian Avendaño-Herrera, Rubén Figueroa, Jaime Rauch, Cecilia Troncoso, José M. Vargas-Chacoff, Luis Yá�ez, Alejandro J. |
spellingShingle |
Pontigo, Juan Pablo Espinoza, Carla Hernandez, Mauricio Nourdin, Guillermo Oliver, Cristian Avendaño-Herrera, Rubén Figueroa, Jaime Rauch, Cecilia Troncoso, José M. Vargas-Chacoff, Luis Yá�ez, Alejandro J. Protein-Based Vaccine Protect Against Piscirickettsia salmonis in Atlantic Salmon (Salmo salar) |
author_facet |
Pontigo, Juan Pablo Espinoza, Carla Hernandez, Mauricio Nourdin, Guillermo Oliver, Cristian Avendaño-Herrera, Rubén Figueroa, Jaime Rauch, Cecilia Troncoso, José M. Vargas-Chacoff, Luis Yá�ez, Alejandro J. |
author_sort |
Pontigo, Juan Pablo |
title |
Protein-Based Vaccine Protect Against Piscirickettsia salmonis in Atlantic Salmon (Salmo salar) |
title_short |
Protein-Based Vaccine Protect Against Piscirickettsia salmonis in Atlantic Salmon (Salmo salar) |
title_full |
Protein-Based Vaccine Protect Against Piscirickettsia salmonis in Atlantic Salmon (Salmo salar) |
title_fullStr |
Protein-Based Vaccine Protect Against Piscirickettsia salmonis in Atlantic Salmon (Salmo salar) |
title_full_unstemmed |
Protein-Based Vaccine Protect Against Piscirickettsia salmonis in Atlantic Salmon (Salmo salar) |
title_sort |
protein-based vaccine protect against piscirickettsia salmonis in atlantic salmon (salmo salar) |
publisher |
Frontiers Media SA |
publishDate |
2021 |
url |
http://dx.doi.org/10.3389/fimmu.2021.602689 https://www.frontiersin.org/articles/10.3389/fimmu.2021.602689/full |
geographic |
Austral |
geographic_facet |
Austral |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Frontiers in Immunology volume 12 ISSN 1664-3224 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fimmu.2021.602689 |
container_title |
Frontiers in Immunology |
container_volume |
12 |
_version_ |
1812175533730955264 |