Innovation in Nucleotide-Binding Oligomerization-Like Receptor and Toll-Like Receptor Sensing Drives the Major Histocompatibility Complex-II Free Atlantic Cod Immune System
The absence of MHC class II antigen presentation and multiple pathogen recognition receptors in the Atlantic cod has not impaired its immune response however how underlying mechanisms have adapted remains largely unknown. In this study, ex vivo cod macrophages were challenged with various bacterial...
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Online Access: | http://dx.doi.org/10.3389/fimmu.2020.609456 https://www.frontiersin.org/articles/10.3389/fimmu.2020.609456/full |
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crfrontiers:10.3389/fimmu.2020.609456 2024-03-31T07:51:26+00:00 Innovation in Nucleotide-Binding Oligomerization-Like Receptor and Toll-Like Receptor Sensing Drives the Major Histocompatibility Complex-II Free Atlantic Cod Immune System Jin, Xingkun Morro, Bernat Tørresen, Ole K. Moiche, Visila Solbakken, Monica H. Jakobsen, Kjetill S. Jentoft, Sissel MacKenzie, Simon 2020 http://dx.doi.org/10.3389/fimmu.2020.609456 https://www.frontiersin.org/articles/10.3389/fimmu.2020.609456/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Immunology volume 11 ISSN 1664-3224 Immunology Immunology and Allergy journal-article 2020 crfrontiers https://doi.org/10.3389/fimmu.2020.609456 2024-03-05T00:17:59Z The absence of MHC class II antigen presentation and multiple pathogen recognition receptors in the Atlantic cod has not impaired its immune response however how underlying mechanisms have adapted remains largely unknown. In this study, ex vivo cod macrophages were challenged with various bacterial and viral microbe-associated molecular patterns (MAMP) to identify major response pathways. Cytosolic MAMP-PRR pathways based upon the NOD-like receptors (NLRs) and RIG-I-like receptors (RLRs) were identified as the critical response pathways. Our analyses suggest that internalization of exogenous ligands through scavenger receptors drives both pathways activating transcription factors like NF-kB (Nuclear factor-kappa B) and interferon regulatory factors (IRFs). Further, ligand-dependent differential expression of a unique TLR25 isoform and multiple NLR paralogues suggests (sub)neofunctionalization toward specific immune defensive strategies. Our results further demonstrate that the unique immune system of the Atlantic cod provides an unprecedented opportunity to explore the evolutionary history of PRR-based signaling in vertebrate immunity. Article in Journal/Newspaper atlantic cod Frontiers (Publisher) Frontiers in Immunology 11 |
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topic |
Immunology Immunology and Allergy |
spellingShingle |
Immunology Immunology and Allergy Jin, Xingkun Morro, Bernat Tørresen, Ole K. Moiche, Visila Solbakken, Monica H. Jakobsen, Kjetill S. Jentoft, Sissel MacKenzie, Simon Innovation in Nucleotide-Binding Oligomerization-Like Receptor and Toll-Like Receptor Sensing Drives the Major Histocompatibility Complex-II Free Atlantic Cod Immune System |
topic_facet |
Immunology Immunology and Allergy |
description |
The absence of MHC class II antigen presentation and multiple pathogen recognition receptors in the Atlantic cod has not impaired its immune response however how underlying mechanisms have adapted remains largely unknown. In this study, ex vivo cod macrophages were challenged with various bacterial and viral microbe-associated molecular patterns (MAMP) to identify major response pathways. Cytosolic MAMP-PRR pathways based upon the NOD-like receptors (NLRs) and RIG-I-like receptors (RLRs) were identified as the critical response pathways. Our analyses suggest that internalization of exogenous ligands through scavenger receptors drives both pathways activating transcription factors like NF-kB (Nuclear factor-kappa B) and interferon regulatory factors (IRFs). Further, ligand-dependent differential expression of a unique TLR25 isoform and multiple NLR paralogues suggests (sub)neofunctionalization toward specific immune defensive strategies. Our results further demonstrate that the unique immune system of the Atlantic cod provides an unprecedented opportunity to explore the evolutionary history of PRR-based signaling in vertebrate immunity. |
format |
Article in Journal/Newspaper |
author |
Jin, Xingkun Morro, Bernat Tørresen, Ole K. Moiche, Visila Solbakken, Monica H. Jakobsen, Kjetill S. Jentoft, Sissel MacKenzie, Simon |
author_facet |
Jin, Xingkun Morro, Bernat Tørresen, Ole K. Moiche, Visila Solbakken, Monica H. Jakobsen, Kjetill S. Jentoft, Sissel MacKenzie, Simon |
author_sort |
Jin, Xingkun |
title |
Innovation in Nucleotide-Binding Oligomerization-Like Receptor and Toll-Like Receptor Sensing Drives the Major Histocompatibility Complex-II Free Atlantic Cod Immune System |
title_short |
Innovation in Nucleotide-Binding Oligomerization-Like Receptor and Toll-Like Receptor Sensing Drives the Major Histocompatibility Complex-II Free Atlantic Cod Immune System |
title_full |
Innovation in Nucleotide-Binding Oligomerization-Like Receptor and Toll-Like Receptor Sensing Drives the Major Histocompatibility Complex-II Free Atlantic Cod Immune System |
title_fullStr |
Innovation in Nucleotide-Binding Oligomerization-Like Receptor and Toll-Like Receptor Sensing Drives the Major Histocompatibility Complex-II Free Atlantic Cod Immune System |
title_full_unstemmed |
Innovation in Nucleotide-Binding Oligomerization-Like Receptor and Toll-Like Receptor Sensing Drives the Major Histocompatibility Complex-II Free Atlantic Cod Immune System |
title_sort |
innovation in nucleotide-binding oligomerization-like receptor and toll-like receptor sensing drives the major histocompatibility complex-ii free atlantic cod immune system |
publisher |
Frontiers Media SA |
publishDate |
2020 |
url |
http://dx.doi.org/10.3389/fimmu.2020.609456 https://www.frontiersin.org/articles/10.3389/fimmu.2020.609456/full |
genre |
atlantic cod |
genre_facet |
atlantic cod |
op_source |
Frontiers in Immunology volume 11 ISSN 1664-3224 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fimmu.2020.609456 |
container_title |
Frontiers in Immunology |
container_volume |
11 |
_version_ |
1795030051684614144 |