Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.)
DNA demethylases (dMTases) are essential proteins in plants that regulate DNA methylation levels. The dMTase genes have been explored in a number of plant species, however, members of this family have not been reported in wheat. We identified 12 wheat dMTase genes divided into two subfamilies: repre...
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crfrontiers:10.3389/fgene.2022.894020 2024-04-28T08:17:08+00:00 Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.) Gahlaut, Vijay Samtani, Harsha Gautam, Tinku Khurana, Paramjit 2022 http://dx.doi.org/10.3389/fgene.2022.894020 https://www.frontiersin.org/articles/10.3389/fgene.2022.894020/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Genetics volume 13 ISSN 1664-8021 Genetics (clinical) Genetics Molecular Medicine journal-article 2022 crfrontiers https://doi.org/10.3389/fgene.2022.894020 2024-04-02T07:43:37Z DNA demethylases (dMTases) are essential proteins in plants that regulate DNA methylation levels. The dMTase genes have been explored in a number of plant species, however, members of this family have not been reported in wheat. We identified 12 wheat dMTase genes divided into two subfamilies: repressor of silencing 1 (ROS1) and DEMETER-Like (DML). The TadMTases in the same subfamily or clade in the phylogenetic tree have similar gene structures, protein motifs, and domains. The promoter sequence contains multiple cis -regulatory elements (CREs) that respond to abiotic stress, hormones, and light, suggesting that the majority of TadMTase genes play a role in wheat growth, development, and stress response. The nuclear localization signals (NLSs), subcellular localization, and SRR motifs were also analyzed. The expression profile analyses revealed that TadMTase genes showed differential gene expression patterns in distinct developmental stages and tissues as well as under heat stress (HS). Furthermore, the qRT-PCR analysis revealed that TadMTase gene expression differed amongst wheat cultivars with varying degrees of HS tolerance. Overall, this work contributes to the understanding of the biological function of wheat dMTases and lays the foundation for future investigations. Article in Journal/Newspaper DML Frontiers (Publisher) Frontiers in Genetics 13 |
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Genetics (clinical) Genetics Molecular Medicine |
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Genetics (clinical) Genetics Molecular Medicine Gahlaut, Vijay Samtani, Harsha Gautam, Tinku Khurana, Paramjit Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.) |
topic_facet |
Genetics (clinical) Genetics Molecular Medicine |
description |
DNA demethylases (dMTases) are essential proteins in plants that regulate DNA methylation levels. The dMTase genes have been explored in a number of plant species, however, members of this family have not been reported in wheat. We identified 12 wheat dMTase genes divided into two subfamilies: repressor of silencing 1 (ROS1) and DEMETER-Like (DML). The TadMTases in the same subfamily or clade in the phylogenetic tree have similar gene structures, protein motifs, and domains. The promoter sequence contains multiple cis -regulatory elements (CREs) that respond to abiotic stress, hormones, and light, suggesting that the majority of TadMTase genes play a role in wheat growth, development, and stress response. The nuclear localization signals (NLSs), subcellular localization, and SRR motifs were also analyzed. The expression profile analyses revealed that TadMTase genes showed differential gene expression patterns in distinct developmental stages and tissues as well as under heat stress (HS). Furthermore, the qRT-PCR analysis revealed that TadMTase gene expression differed amongst wheat cultivars with varying degrees of HS tolerance. Overall, this work contributes to the understanding of the biological function of wheat dMTases and lays the foundation for future investigations. |
format |
Article in Journal/Newspaper |
author |
Gahlaut, Vijay Samtani, Harsha Gautam, Tinku Khurana, Paramjit |
author_facet |
Gahlaut, Vijay Samtani, Harsha Gautam, Tinku Khurana, Paramjit |
author_sort |
Gahlaut, Vijay |
title |
Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.) |
title_short |
Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.) |
title_full |
Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.) |
title_fullStr |
Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.) |
title_full_unstemmed |
Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.) |
title_sort |
identification and characterization of dna demethylase genes and their association with thermal stress in wheat (triticum aestivum l.) |
publisher |
Frontiers Media SA |
publishDate |
2022 |
url |
http://dx.doi.org/10.3389/fgene.2022.894020 https://www.frontiersin.org/articles/10.3389/fgene.2022.894020/full |
genre |
DML |
genre_facet |
DML |
op_source |
Frontiers in Genetics volume 13 ISSN 1664-8021 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fgene.2022.894020 |
container_title |
Frontiers in Genetics |
container_volume |
13 |
_version_ |
1797581907864584192 |