First Description of a Satellite DNA in Manatees’ Centromeric Regions
Trichechus manatus and Trichechus inunguis are the two Sirenia species that occur in the Americas. Despite their increasing extinction risk, many aspects of their biology remain understudied, including the repetitive DNA fraction of their genomes. Here we used the sequenced genome of T. manatus and...
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Online Access: | http://dx.doi.org/10.3389/fgene.2021.694866 https://www.frontiersin.org/articles/10.3389/fgene.2021.694866/full |
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crfrontiers:10.3389/fgene.2021.694866 2024-10-06T13:49:23+00:00 First Description of a Satellite DNA in Manatees’ Centromeric Regions Valeri, Mirela Pelizaro Dias, Guilherme Borges do Espírito Santo, Alice Alves Moreira, Camila Nascimento Yonenaga-Yassuda, Yatiyo Sommer, Iara Braga Kuhn, Gustavo C. S. Svartman, Marta Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Conselho Nacional de Desenvolvimento Científico e Tecnológico 2021 http://dx.doi.org/10.3389/fgene.2021.694866 https://www.frontiersin.org/articles/10.3389/fgene.2021.694866/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Genetics volume 12 ISSN 1664-8021 journal-article 2021 crfrontiers https://doi.org/10.3389/fgene.2021.694866 2024-09-10T04:04:08Z Trichechus manatus and Trichechus inunguis are the two Sirenia species that occur in the Americas. Despite their increasing extinction risk, many aspects of their biology remain understudied, including the repetitive DNA fraction of their genomes. Here we used the sequenced genome of T. manatus and TAREAN to identify satellite DNAs (satDNAs) in this species. We report the first description of TMAsat, a satDNA comprising ~0.87% of the genome, with ~684bp monomers and centromeric localization. In T. inunguis , TMAsat showed similar monomer length, chromosome localization and conserved CENP-B box-like motifs as in T. manatus . We also detected this satDNA in the Dugong dugon and in the now extinct Hydrodamalis gigas genomes. The neighbor-joining tree shows that TMAsat sequences from T. manatus , T. inunguis , D. dugon , and H. gigas lack species-specific clusters, which disagrees with the predictions of concerted evolution. We detected a divergent TMAsat-like homologous sequence in elephants and hyraxes, but not in other mammals, suggesting this sequence was already present in the common ancestor of Paenungulata, and later became a satDNA in the Sirenians. This is the first description of a centromeric satDNA in manatees and will facilitate the inclusion of Sirenia in future studies of centromeres and satDNA biology. Article in Journal/Newspaper Hydrodamalis gigas Frontiers (Publisher) Frontiers in Genetics 12 |
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Trichechus manatus and Trichechus inunguis are the two Sirenia species that occur in the Americas. Despite their increasing extinction risk, many aspects of their biology remain understudied, including the repetitive DNA fraction of their genomes. Here we used the sequenced genome of T. manatus and TAREAN to identify satellite DNAs (satDNAs) in this species. We report the first description of TMAsat, a satDNA comprising ~0.87% of the genome, with ~684bp monomers and centromeric localization. In T. inunguis , TMAsat showed similar monomer length, chromosome localization and conserved CENP-B box-like motifs as in T. manatus . We also detected this satDNA in the Dugong dugon and in the now extinct Hydrodamalis gigas genomes. The neighbor-joining tree shows that TMAsat sequences from T. manatus , T. inunguis , D. dugon , and H. gigas lack species-specific clusters, which disagrees with the predictions of concerted evolution. We detected a divergent TMAsat-like homologous sequence in elephants and hyraxes, but not in other mammals, suggesting this sequence was already present in the common ancestor of Paenungulata, and later became a satDNA in the Sirenians. This is the first description of a centromeric satDNA in manatees and will facilitate the inclusion of Sirenia in future studies of centromeres and satDNA biology. |
author2 |
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Conselho Nacional de Desenvolvimento Científico e Tecnológico |
format |
Article in Journal/Newspaper |
author |
Valeri, Mirela Pelizaro Dias, Guilherme Borges do Espírito Santo, Alice Alves Moreira, Camila Nascimento Yonenaga-Yassuda, Yatiyo Sommer, Iara Braga Kuhn, Gustavo C. S. Svartman, Marta |
spellingShingle |
Valeri, Mirela Pelizaro Dias, Guilherme Borges do Espírito Santo, Alice Alves Moreira, Camila Nascimento Yonenaga-Yassuda, Yatiyo Sommer, Iara Braga Kuhn, Gustavo C. S. Svartman, Marta First Description of a Satellite DNA in Manatees’ Centromeric Regions |
author_facet |
Valeri, Mirela Pelizaro Dias, Guilherme Borges do Espírito Santo, Alice Alves Moreira, Camila Nascimento Yonenaga-Yassuda, Yatiyo Sommer, Iara Braga Kuhn, Gustavo C. S. Svartman, Marta |
author_sort |
Valeri, Mirela Pelizaro |
title |
First Description of a Satellite DNA in Manatees’ Centromeric Regions |
title_short |
First Description of a Satellite DNA in Manatees’ Centromeric Regions |
title_full |
First Description of a Satellite DNA in Manatees’ Centromeric Regions |
title_fullStr |
First Description of a Satellite DNA in Manatees’ Centromeric Regions |
title_full_unstemmed |
First Description of a Satellite DNA in Manatees’ Centromeric Regions |
title_sort |
first description of a satellite dna in manatees’ centromeric regions |
publisher |
Frontiers Media SA |
publishDate |
2021 |
url |
http://dx.doi.org/10.3389/fgene.2021.694866 https://www.frontiersin.org/articles/10.3389/fgene.2021.694866/full |
genre |
Hydrodamalis gigas |
genre_facet |
Hydrodamalis gigas |
op_source |
Frontiers in Genetics volume 12 ISSN 1664-8021 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fgene.2021.694866 |
container_title |
Frontiers in Genetics |
container_volume |
12 |
_version_ |
1812177445865914368 |