Differential Regulation of the Expression of the Two Thyrotropin Beta Subunit Paralogs by Salmon Pituitary Cells In Vitro
We recently characterized two paralogs of the thyrotropin (TSH) beta subunit in Atlantic salmon, tshβa and tshβb , issued from teleost-specific whole genome duplication. The transcript expression of tshβb , but not of tshβa , peaks at the time of smoltification, which revealed a specific involvement...
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Online Access: | http://dx.doi.org/10.3389/fendo.2020.603538 https://www.frontiersin.org/articles/10.3389/fendo.2020.603538/full |
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crfrontiers:10.3389/fendo.2020.603538 2024-09-15T17:56:20+00:00 Differential Regulation of the Expression of the Two Thyrotropin Beta Subunit Paralogs by Salmon Pituitary Cells In Vitro Fleming, Mitchell Stewart Maugars, Gersende Martin, Patrick Dufour, Sylvie Rousseau, Karine Agence Nationale de la Recherche FP7 People: Marie-Curie Actions 2020 http://dx.doi.org/10.3389/fendo.2020.603538 https://www.frontiersin.org/articles/10.3389/fendo.2020.603538/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Endocrinology volume 11 ISSN 1664-2392 journal-article 2020 crfrontiers https://doi.org/10.3389/fendo.2020.603538 2024-07-23T04:02:53Z We recently characterized two paralogs of the thyrotropin (TSH) beta subunit in Atlantic salmon, tshβa and tshβb , issued from teleost-specific whole genome duplication. The transcript expression of tshβb , but not of tshβa , peaks at the time of smoltification, which revealed a specific involvement of tshβb paralog in this metamorphic event. Tshβa and tshβb are expressed by distinct pituitary cells in salmon, likely related to TSH cells from the pars distalis and pars tuberalis , respectively, in mammals and birds. The present study aimed at investigating the neuroendocrine and endocrine factors potentially involved in the differential regulation of tshβa and tshβb paralogs, using primary cultures of Atlantic salmon pituitary cells. The effects of various neurohormones and endocrine factors potentially involved in the control of development, growth, and metabolism were tested. Transcript levels of tshβa and tshβb were measured by qPCR, as well as those of growth hormone ( gh ), for comparison and validation. Corticotropin-releasing hormone (CRH) stimulated tshβa transcript levels in agreement with its potential role in the thyrotropic axis in teleosts, but had no effect on tshβb paralog, while it also stimulated gh transcript levels. Thyrotropin-releasing hormone (TRH) had no effect on neither tshβ paralogs nor gh . Somatostatin (SRIH) had no effects on both tshβ paralogs, while it exerted a canonical inhibitory effect on gh transcript levels. Thyroid hormones [triiodothyronine (T3) and thyroxine (T4)] inhibited transcript levels of both tshβ paralogs, as well as gh , but with a much stronger effect on tshβa than on tshβb and gh. Conversely, cortisol had a stronger inhibitory effect on tshβb than tshβa , while no effect on gh . Remarkably, insulin-like growth factor 1 (IGF1) dose-dependently stimulated tshβb transcript levels, while it had no effect on tshβa , and a classical inhibitory effect on gh . This study provides the first data on the neuroendocrine factors involved in the differential regulation of the ... Article in Journal/Newspaper Atlantic salmon Frontiers (Publisher) Frontiers in Endocrinology 11 |
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We recently characterized two paralogs of the thyrotropin (TSH) beta subunit in Atlantic salmon, tshβa and tshβb , issued from teleost-specific whole genome duplication. The transcript expression of tshβb , but not of tshβa , peaks at the time of smoltification, which revealed a specific involvement of tshβb paralog in this metamorphic event. Tshβa and tshβb are expressed by distinct pituitary cells in salmon, likely related to TSH cells from the pars distalis and pars tuberalis , respectively, in mammals and birds. The present study aimed at investigating the neuroendocrine and endocrine factors potentially involved in the differential regulation of tshβa and tshβb paralogs, using primary cultures of Atlantic salmon pituitary cells. The effects of various neurohormones and endocrine factors potentially involved in the control of development, growth, and metabolism were tested. Transcript levels of tshβa and tshβb were measured by qPCR, as well as those of growth hormone ( gh ), for comparison and validation. Corticotropin-releasing hormone (CRH) stimulated tshβa transcript levels in agreement with its potential role in the thyrotropic axis in teleosts, but had no effect on tshβb paralog, while it also stimulated gh transcript levels. Thyrotropin-releasing hormone (TRH) had no effect on neither tshβ paralogs nor gh . Somatostatin (SRIH) had no effects on both tshβ paralogs, while it exerted a canonical inhibitory effect on gh transcript levels. Thyroid hormones [triiodothyronine (T3) and thyroxine (T4)] inhibited transcript levels of both tshβ paralogs, as well as gh , but with a much stronger effect on tshβa than on tshβb and gh. Conversely, cortisol had a stronger inhibitory effect on tshβb than tshβa , while no effect on gh . Remarkably, insulin-like growth factor 1 (IGF1) dose-dependently stimulated tshβb transcript levels, while it had no effect on tshβa , and a classical inhibitory effect on gh . This study provides the first data on the neuroendocrine factors involved in the differential regulation of the ... |
author2 |
Agence Nationale de la Recherche FP7 People: Marie-Curie Actions |
format |
Article in Journal/Newspaper |
author |
Fleming, Mitchell Stewart Maugars, Gersende Martin, Patrick Dufour, Sylvie Rousseau, Karine |
spellingShingle |
Fleming, Mitchell Stewart Maugars, Gersende Martin, Patrick Dufour, Sylvie Rousseau, Karine Differential Regulation of the Expression of the Two Thyrotropin Beta Subunit Paralogs by Salmon Pituitary Cells In Vitro |
author_facet |
Fleming, Mitchell Stewart Maugars, Gersende Martin, Patrick Dufour, Sylvie Rousseau, Karine |
author_sort |
Fleming, Mitchell Stewart |
title |
Differential Regulation of the Expression of the Two Thyrotropin Beta Subunit Paralogs by Salmon Pituitary Cells In Vitro |
title_short |
Differential Regulation of the Expression of the Two Thyrotropin Beta Subunit Paralogs by Salmon Pituitary Cells In Vitro |
title_full |
Differential Regulation of the Expression of the Two Thyrotropin Beta Subunit Paralogs by Salmon Pituitary Cells In Vitro |
title_fullStr |
Differential Regulation of the Expression of the Two Thyrotropin Beta Subunit Paralogs by Salmon Pituitary Cells In Vitro |
title_full_unstemmed |
Differential Regulation of the Expression of the Two Thyrotropin Beta Subunit Paralogs by Salmon Pituitary Cells In Vitro |
title_sort |
differential regulation of the expression of the two thyrotropin beta subunit paralogs by salmon pituitary cells in vitro |
publisher |
Frontiers Media SA |
publishDate |
2020 |
url |
http://dx.doi.org/10.3389/fendo.2020.603538 https://www.frontiersin.org/articles/10.3389/fendo.2020.603538/full |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Frontiers in Endocrinology volume 11 ISSN 1664-2392 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fendo.2020.603538 |
container_title |
Frontiers in Endocrinology |
container_volume |
11 |
_version_ |
1810432540796780544 |