Cardiomyocyte Protection by Hibernating Brown Bear Serum: Toward the Identification of New Protective Molecules Against Myocardial Infarction
Ischemic heart disease remains one of the leading causes of death worldwide. Despite intensive research on the treatment of acute myocardial infarction, no effective therapy has shown clinical success. Therefore, novel therapeutic strategies are required to protect the heart from reperfusion injury....
Published in: | Frontiers in Cardiovascular Medicine |
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Online Access: | http://dx.doi.org/10.3389/fcvm.2021.687501 https://www.frontiersin.org/articles/10.3389/fcvm.2021.687501/full |
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crfrontiers:10.3389/fcvm.2021.687501 2024-09-30T14:45:37+00:00 Cardiomyocyte Protection by Hibernating Brown Bear Serum: Toward the Identification of New Protective Molecules Against Myocardial Infarction Givre, Lucas Crola Da Silva, Claire Swenson, Jon E. Arnemo, Jon M. Gauquelin-Koch, Guillemette Bertile, Fabrice Lefai, Etienne Gomez, Ludovic 2021 http://dx.doi.org/10.3389/fcvm.2021.687501 https://www.frontiersin.org/articles/10.3389/fcvm.2021.687501/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Cardiovascular Medicine volume 8 ISSN 2297-055X journal-article 2021 crfrontiers https://doi.org/10.3389/fcvm.2021.687501 2024-09-03T04:05:56Z Ischemic heart disease remains one of the leading causes of death worldwide. Despite intensive research on the treatment of acute myocardial infarction, no effective therapy has shown clinical success. Therefore, novel therapeutic strategies are required to protect the heart from reperfusion injury. Interestingly, despite physical inactivity during hibernation, brown bears ( Ursus arctos ) cope with cardiovascular physiological conditions that would be detrimental to humans. We hypothesized that bear serum might contain circulating factors that could provide protection against cell injury. In this study, we sought to determine whether addition of bear serum might improve cardiomyocyte survival following hypoxia–reoxygenation. Isolated mouse cardiomyocytes underwent 45 min of hypoxia followed by reoxygenation. At the onset of reoxygenation, cells received fetal bovine serum (FBS; positive control), summer (SBS) or winter bear serum (WBS), or adult serums of other species, as indicated. After 2 h of reoxygenation, propidium iodide staining was used to evaluate cell viability by flow cytometry. Whereas, 0.5% SBS tended to decrease reperfusion injury, 0.5% WBS significantly reduced cell death, averaging 74.04 ± 7.06% vs. 79.20 ± 6.53% in the FBS group. This cardioprotective effect was lost at 0.1%, became toxic above 5%, and was specific to the bear. Our results showed that bear serum exerts a therapeutic effect with an efficacy threshold, an optimal dose, and a toxic effect on cardiomyocyte viability after hypoxia–reoxygenation. Therefore, the bear serum may be a potential source for identifying new therapeutic molecules to fight against myocardial reperfusion injury and cell death in general. Article in Journal/Newspaper Ursus arctos Frontiers (Publisher) Frontiers in Cardiovascular Medicine 8 |
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Ischemic heart disease remains one of the leading causes of death worldwide. Despite intensive research on the treatment of acute myocardial infarction, no effective therapy has shown clinical success. Therefore, novel therapeutic strategies are required to protect the heart from reperfusion injury. Interestingly, despite physical inactivity during hibernation, brown bears ( Ursus arctos ) cope with cardiovascular physiological conditions that would be detrimental to humans. We hypothesized that bear serum might contain circulating factors that could provide protection against cell injury. In this study, we sought to determine whether addition of bear serum might improve cardiomyocyte survival following hypoxia–reoxygenation. Isolated mouse cardiomyocytes underwent 45 min of hypoxia followed by reoxygenation. At the onset of reoxygenation, cells received fetal bovine serum (FBS; positive control), summer (SBS) or winter bear serum (WBS), or adult serums of other species, as indicated. After 2 h of reoxygenation, propidium iodide staining was used to evaluate cell viability by flow cytometry. Whereas, 0.5% SBS tended to decrease reperfusion injury, 0.5% WBS significantly reduced cell death, averaging 74.04 ± 7.06% vs. 79.20 ± 6.53% in the FBS group. This cardioprotective effect was lost at 0.1%, became toxic above 5%, and was specific to the bear. Our results showed that bear serum exerts a therapeutic effect with an efficacy threshold, an optimal dose, and a toxic effect on cardiomyocyte viability after hypoxia–reoxygenation. Therefore, the bear serum may be a potential source for identifying new therapeutic molecules to fight against myocardial reperfusion injury and cell death in general. |
format |
Article in Journal/Newspaper |
author |
Givre, Lucas Crola Da Silva, Claire Swenson, Jon E. Arnemo, Jon M. Gauquelin-Koch, Guillemette Bertile, Fabrice Lefai, Etienne Gomez, Ludovic |
spellingShingle |
Givre, Lucas Crola Da Silva, Claire Swenson, Jon E. Arnemo, Jon M. Gauquelin-Koch, Guillemette Bertile, Fabrice Lefai, Etienne Gomez, Ludovic Cardiomyocyte Protection by Hibernating Brown Bear Serum: Toward the Identification of New Protective Molecules Against Myocardial Infarction |
author_facet |
Givre, Lucas Crola Da Silva, Claire Swenson, Jon E. Arnemo, Jon M. Gauquelin-Koch, Guillemette Bertile, Fabrice Lefai, Etienne Gomez, Ludovic |
author_sort |
Givre, Lucas |
title |
Cardiomyocyte Protection by Hibernating Brown Bear Serum: Toward the Identification of New Protective Molecules Against Myocardial Infarction |
title_short |
Cardiomyocyte Protection by Hibernating Brown Bear Serum: Toward the Identification of New Protective Molecules Against Myocardial Infarction |
title_full |
Cardiomyocyte Protection by Hibernating Brown Bear Serum: Toward the Identification of New Protective Molecules Against Myocardial Infarction |
title_fullStr |
Cardiomyocyte Protection by Hibernating Brown Bear Serum: Toward the Identification of New Protective Molecules Against Myocardial Infarction |
title_full_unstemmed |
Cardiomyocyte Protection by Hibernating Brown Bear Serum: Toward the Identification of New Protective Molecules Against Myocardial Infarction |
title_sort |
cardiomyocyte protection by hibernating brown bear serum: toward the identification of new protective molecules against myocardial infarction |
publisher |
Frontiers Media SA |
publishDate |
2021 |
url |
http://dx.doi.org/10.3389/fcvm.2021.687501 https://www.frontiersin.org/articles/10.3389/fcvm.2021.687501/full |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_source |
Frontiers in Cardiovascular Medicine volume 8 ISSN 2297-055X |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fcvm.2021.687501 |
container_title |
Frontiers in Cardiovascular Medicine |
container_volume |
8 |
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1811646163978289152 |