Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection
Mortality outbreaks of young Pacific oysters, Crassostrea gigas , have seriously affected the oyster-farming economy in several countries around the world. Although the causes of these mortality outbreaks appear complex, a viral agent has been identified as the main factor: a herpesvirus called ostr...
Published in: | Frontiers in Cellular and Infection Microbiology |
---|---|
Main Authors: | , , , , , , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Frontiers Media SA
2022
|
Subjects: | |
Online Access: | http://dx.doi.org/10.3389/fcimb.2022.858311 https://www.frontiersin.org/articles/10.3389/fcimb.2022.858311/full |
id |
crfrontiers:10.3389/fcimb.2022.858311 |
---|---|
record_format |
openpolar |
spelling |
crfrontiers:10.3389/fcimb.2022.858311 2024-05-19T07:39:16+00:00 Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection Picot, Sandy Faury, Nicole Pelletier, Camille Arzul, Isabelle Chollet, Bruno Dégremont, Lionel Renault, Tristan Morga, Benjamin H2020 Excellent Science 2022 http://dx.doi.org/10.3389/fcimb.2022.858311 https://www.frontiersin.org/articles/10.3389/fcimb.2022.858311/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Cellular and Infection Microbiology volume 12 ISSN 2235-2988 journal-article 2022 crfrontiers https://doi.org/10.3389/fcimb.2022.858311 2024-05-01T06:50:19Z Mortality outbreaks of young Pacific oysters, Crassostrea gigas , have seriously affected the oyster-farming economy in several countries around the world. Although the causes of these mortality outbreaks appear complex, a viral agent has been identified as the main factor: a herpesvirus called ostreid herpesvirus 1 (OsHV-1). Autophagy is an important degradation pathway involved in the response to several pathologies including viral diseases. In C. gigas , recent studies indicate that this pathway is conserved and functional in at least haemocytes and the mantle. Furthermore, an experimental infection in combination with compounds known to inhibit or induce autophagy in mammals revealed that autophagy is involved in the response to OsHV-1 infection. In light of these results, the aim of this study was to determine the role of autophagy in the response of the Pacific oyster to infection by virus OsHV-1. For this purpose, an experimental infection in combination with a modulator of autophagy was performed on Pacific oysters known to have intermediate susceptibility to OsHV-1 infection. In haemolymph and the mantle, the autophagy response was monitored by flow cytometry, western blotting, and real-time PCR. At the same time, viral infection was evaluated by quantifying viral DNA and RNA amounts by real-time PCR. Although the results showed activation of autophagy in haemolymph and the mantle 14 hours post infection (after viral replication was initiated), they were also indicative of different regulatory mechanisms of autophagy in the two tissues, thus supporting an important function of autophagy in the response to virus OsHV-1. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Frontiers (Publisher) Frontiers in Cellular and Infection Microbiology 12 |
institution |
Open Polar |
collection |
Frontiers (Publisher) |
op_collection_id |
crfrontiers |
language |
unknown |
description |
Mortality outbreaks of young Pacific oysters, Crassostrea gigas , have seriously affected the oyster-farming economy in several countries around the world. Although the causes of these mortality outbreaks appear complex, a viral agent has been identified as the main factor: a herpesvirus called ostreid herpesvirus 1 (OsHV-1). Autophagy is an important degradation pathway involved in the response to several pathologies including viral diseases. In C. gigas , recent studies indicate that this pathway is conserved and functional in at least haemocytes and the mantle. Furthermore, an experimental infection in combination with compounds known to inhibit or induce autophagy in mammals revealed that autophagy is involved in the response to OsHV-1 infection. In light of these results, the aim of this study was to determine the role of autophagy in the response of the Pacific oyster to infection by virus OsHV-1. For this purpose, an experimental infection in combination with a modulator of autophagy was performed on Pacific oysters known to have intermediate susceptibility to OsHV-1 infection. In haemolymph and the mantle, the autophagy response was monitored by flow cytometry, western blotting, and real-time PCR. At the same time, viral infection was evaluated by quantifying viral DNA and RNA amounts by real-time PCR. Although the results showed activation of autophagy in haemolymph and the mantle 14 hours post infection (after viral replication was initiated), they were also indicative of different regulatory mechanisms of autophagy in the two tissues, thus supporting an important function of autophagy in the response to virus OsHV-1. |
author2 |
H2020 Excellent Science |
format |
Article in Journal/Newspaper |
author |
Picot, Sandy Faury, Nicole Pelletier, Camille Arzul, Isabelle Chollet, Bruno Dégremont, Lionel Renault, Tristan Morga, Benjamin |
spellingShingle |
Picot, Sandy Faury, Nicole Pelletier, Camille Arzul, Isabelle Chollet, Bruno Dégremont, Lionel Renault, Tristan Morga, Benjamin Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection |
author_facet |
Picot, Sandy Faury, Nicole Pelletier, Camille Arzul, Isabelle Chollet, Bruno Dégremont, Lionel Renault, Tristan Morga, Benjamin |
author_sort |
Picot, Sandy |
title |
Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection |
title_short |
Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection |
title_full |
Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection |
title_fullStr |
Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection |
title_full_unstemmed |
Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection |
title_sort |
monitoring autophagy at cellular and molecular level in crassostrea gigas during an experimental ostreid herpesvirus 1 (oshv-1) infection |
publisher |
Frontiers Media SA |
publishDate |
2022 |
url |
http://dx.doi.org/10.3389/fcimb.2022.858311 https://www.frontiersin.org/articles/10.3389/fcimb.2022.858311/full |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Frontiers in Cellular and Infection Microbiology volume 12 ISSN 2235-2988 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fcimb.2022.858311 |
container_title |
Frontiers in Cellular and Infection Microbiology |
container_volume |
12 |
_version_ |
1799478826700374016 |