Complete Lipopolysaccharide of Piscirickettsia salmonis Is Required for Full Virulence in the Intraperitoneally Challenged Atlantic Salmon, Salmo salar, Model
Bacterial cell envelopes play a critical role in host-pathogen interactions. Macromolecular components of these structures have been closely linked to the virulence of pathogens. Piscirickettsia salmonis is a relevant salmonid pathogen with a worldwide distribution. This bacterium is the etiological...
Published in: | Frontiers in Cellular and Infection Microbiology |
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2022
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Online Access: | http://dx.doi.org/10.3389/fcimb.2022.845661 https://www.frontiersin.org/articles/10.3389/fcimb.2022.845661/full |
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crfrontiers:10.3389/fcimb.2022.845661 2024-06-23T07:51:20+00:00 Complete Lipopolysaccharide of Piscirickettsia salmonis Is Required for Full Virulence in the Intraperitoneally Challenged Atlantic Salmon, Salmo salar, Model Herrera, Valeska Olavarría, Nicole Saavedra, José Yuivar, Yassef Bustos, Patricio Almarza, Oscar Mancilla, Marcos Corporación de Fomento de la Producción Corporación de Fomento de la Producción Fondo Nacional de Desarrollo Científico y Tecnológico 2022 http://dx.doi.org/10.3389/fcimb.2022.845661 https://www.frontiersin.org/articles/10.3389/fcimb.2022.845661/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Cellular and Infection Microbiology volume 12 ISSN 2235-2988 journal-article 2022 crfrontiers https://doi.org/10.3389/fcimb.2022.845661 2024-06-11T04:09:27Z Bacterial cell envelopes play a critical role in host-pathogen interactions. Macromolecular components of these structures have been closely linked to the virulence of pathogens. Piscirickettsia salmonis is a relevant salmonid pathogen with a worldwide distribution. This bacterium is the etiological agent of piscirickettsiosis, a septicemic disease that causes a high economic burden, especially for the Chilean salmon farming industry. Although P. salmonis has been discovered long ago, its pathogenicity and virulence mechanisms are not completely understood. In this work, we present a genetic approach for producing in-frame deletion mutants on genes related to the biosynthesis of membrane-associated polysaccharides. We provide a detailed in vitro phenotype description of knock-out mutants on wzx and wcaJ genes, which encode predicted lipopolysaccharide (LPS) flippase and undecaprenyl-phosphate glucose phosphotransferase enzymes, respectively. We exhibit evidence that the wzx mutant strain carries a defect in the probably most external LPS moiety, while the wcaJ mutant proved to be highly susceptible to the bactericidal action of serum but retained the ability of biofilm production. Beyond that, we demonstrate that the deletion of wzx , but not wcaJ , impairs the virulence of P. salmonis in an intraperitoneally infected Atlantic salmon, Salmo salar , model of piscirickettsiosis. Our findings support a role for LPS in the virulence of P. salmonis during the onset of piscirickettsiosis. Article in Journal/Newspaper Atlantic salmon Salmo salar Frontiers (Publisher) Frontiers in Cellular and Infection Microbiology 12 |
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Frontiers (Publisher) |
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Bacterial cell envelopes play a critical role in host-pathogen interactions. Macromolecular components of these structures have been closely linked to the virulence of pathogens. Piscirickettsia salmonis is a relevant salmonid pathogen with a worldwide distribution. This bacterium is the etiological agent of piscirickettsiosis, a septicemic disease that causes a high economic burden, especially for the Chilean salmon farming industry. Although P. salmonis has been discovered long ago, its pathogenicity and virulence mechanisms are not completely understood. In this work, we present a genetic approach for producing in-frame deletion mutants on genes related to the biosynthesis of membrane-associated polysaccharides. We provide a detailed in vitro phenotype description of knock-out mutants on wzx and wcaJ genes, which encode predicted lipopolysaccharide (LPS) flippase and undecaprenyl-phosphate glucose phosphotransferase enzymes, respectively. We exhibit evidence that the wzx mutant strain carries a defect in the probably most external LPS moiety, while the wcaJ mutant proved to be highly susceptible to the bactericidal action of serum but retained the ability of biofilm production. Beyond that, we demonstrate that the deletion of wzx , but not wcaJ , impairs the virulence of P. salmonis in an intraperitoneally infected Atlantic salmon, Salmo salar , model of piscirickettsiosis. Our findings support a role for LPS in the virulence of P. salmonis during the onset of piscirickettsiosis. |
author2 |
Corporación de Fomento de la Producción Corporación de Fomento de la Producción Fondo Nacional de Desarrollo Científico y Tecnológico |
format |
Article in Journal/Newspaper |
author |
Herrera, Valeska Olavarría, Nicole Saavedra, José Yuivar, Yassef Bustos, Patricio Almarza, Oscar Mancilla, Marcos |
spellingShingle |
Herrera, Valeska Olavarría, Nicole Saavedra, José Yuivar, Yassef Bustos, Patricio Almarza, Oscar Mancilla, Marcos Complete Lipopolysaccharide of Piscirickettsia salmonis Is Required for Full Virulence in the Intraperitoneally Challenged Atlantic Salmon, Salmo salar, Model |
author_facet |
Herrera, Valeska Olavarría, Nicole Saavedra, José Yuivar, Yassef Bustos, Patricio Almarza, Oscar Mancilla, Marcos |
author_sort |
Herrera, Valeska |
title |
Complete Lipopolysaccharide of Piscirickettsia salmonis Is Required for Full Virulence in the Intraperitoneally Challenged Atlantic Salmon, Salmo salar, Model |
title_short |
Complete Lipopolysaccharide of Piscirickettsia salmonis Is Required for Full Virulence in the Intraperitoneally Challenged Atlantic Salmon, Salmo salar, Model |
title_full |
Complete Lipopolysaccharide of Piscirickettsia salmonis Is Required for Full Virulence in the Intraperitoneally Challenged Atlantic Salmon, Salmo salar, Model |
title_fullStr |
Complete Lipopolysaccharide of Piscirickettsia salmonis Is Required for Full Virulence in the Intraperitoneally Challenged Atlantic Salmon, Salmo salar, Model |
title_full_unstemmed |
Complete Lipopolysaccharide of Piscirickettsia salmonis Is Required for Full Virulence in the Intraperitoneally Challenged Atlantic Salmon, Salmo salar, Model |
title_sort |
complete lipopolysaccharide of piscirickettsia salmonis is required for full virulence in the intraperitoneally challenged atlantic salmon, salmo salar, model |
publisher |
Frontiers Media SA |
publishDate |
2022 |
url |
http://dx.doi.org/10.3389/fcimb.2022.845661 https://www.frontiersin.org/articles/10.3389/fcimb.2022.845661/full |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Frontiers in Cellular and Infection Microbiology volume 12 ISSN 2235-2988 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fcimb.2022.845661 |
container_title |
Frontiers in Cellular and Infection Microbiology |
container_volume |
12 |
_version_ |
1802642383236497408 |