Chagas Immunochromatographic Rapid Test in the Serological Diagnosis of Trypanosoma cruzi Infection in Wild and Domestic Canids
Canis lupus familiaris (domestic dog) represents a reliable sentinel for the occurrence of a well-established transmission cycle of Trypanosoma cruzi among wild mammals in the surroundings and, consequently, where the risk of human infection exists. Serological diagnosis is the chosen method to iden...
Published in: | Frontiers in Cellular and Infection Microbiology |
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2022
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crfrontiers:10.3389/fcimb.2022.835383 2024-04-28T08:15:33+00:00 Chagas Immunochromatographic Rapid Test in the Serological Diagnosis of Trypanosoma cruzi Infection in Wild and Domestic Canids Rodrigues, Esthefany S. Santos, Gilbert Q. Silva, Marlon Vicente da Barros, Juliana H. S. Bernardo, Aline R. Diniz, Rafaela L. Rubim, Nara M. Roque, André L. R. Jansen, Ana Maria Silva, Edimilson D. Xavier, Samanta C. C. Conselho Nacional de Desenvolvimento Científico e Tecnológico Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro 2022 http://dx.doi.org/10.3389/fcimb.2022.835383 https://www.frontiersin.org/articles/10.3389/fcimb.2022.835383/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Cellular and Infection Microbiology volume 12 ISSN 2235-2988 Infectious Diseases Microbiology (medical) Immunology Microbiology journal-article 2022 crfrontiers https://doi.org/10.3389/fcimb.2022.835383 2024-04-02T07:42:39Z Canis lupus familiaris (domestic dog) represents a reliable sentinel for the occurrence of a well-established transmission cycle of Trypanosoma cruzi among wild mammals in the surroundings and, consequently, where the risk of human infection exists. Serological diagnosis is the chosen method to identify T. cruzi infection in dogs that, in Brazil, rarely present positive parasitological tests. The use of recombinant chimeric parasitic antigens results in a sensitive and specific serological diagnostic test in contrast to the use of crude T. cruzi antigens. Our objective was to evaluate the Chagas/Bio-Manguinhos Lateral Flow Immunochromatographic Rapid Test (Chagas-LFRT) for the diagnosis of T. cruzi infection in domestic dogs and the potential of application of this diagnostic platform to wild canid species. Two recombinant proteins (IBMP-8.1 and IBMP-8.4) that displayed the best performance in the enzyme immunoassay (ELISA) in previous studies were tested in a platform with two diagnostic bands. A panel of 281 dog serum samples was evaluated: 133 positive for T. cruzi by serological diagnosis, including 20 samples with positive blood cultures belonging to different discrete typing units (DTUs); 129 negative samples; and 19 samples from dogs infected by other trypanosomatids: Leishmania infantum , Trypanosoma rangeli , Trypanosoma caninum and Crithidia mellificae , in addition to samples infected by Anaplasma platys , Dirofilaria immitis and Erlichia sp. that were employed to evaluate eventual cross-reactions. We also evaluated the Chagas-LFRT to detect T. cruzi infection in 9 serum samples from six wild canid species. We observed that the intensity pattern of the bands was directly proportional to the serological titer observed in IFAT. The sensitivity was 94%, the specificity was 91% according to the ROC curve, and the defined cutoff was an optical density of 4.8. The agreement obtained was considered substantial by the kappa analysis (84%). From T. cruzi positive hemoculture samples, 88.9% were positive by ... Article in Journal/Newspaper Canis lupus Frontiers (Publisher) Frontiers in Cellular and Infection Microbiology 12 |
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Infectious Diseases Microbiology (medical) Immunology Microbiology |
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Infectious Diseases Microbiology (medical) Immunology Microbiology Rodrigues, Esthefany S. Santos, Gilbert Q. Silva, Marlon Vicente da Barros, Juliana H. S. Bernardo, Aline R. Diniz, Rafaela L. Rubim, Nara M. Roque, André L. R. Jansen, Ana Maria Silva, Edimilson D. Xavier, Samanta C. C. Chagas Immunochromatographic Rapid Test in the Serological Diagnosis of Trypanosoma cruzi Infection in Wild and Domestic Canids |
topic_facet |
Infectious Diseases Microbiology (medical) Immunology Microbiology |
description |
Canis lupus familiaris (domestic dog) represents a reliable sentinel for the occurrence of a well-established transmission cycle of Trypanosoma cruzi among wild mammals in the surroundings and, consequently, where the risk of human infection exists. Serological diagnosis is the chosen method to identify T. cruzi infection in dogs that, in Brazil, rarely present positive parasitological tests. The use of recombinant chimeric parasitic antigens results in a sensitive and specific serological diagnostic test in contrast to the use of crude T. cruzi antigens. Our objective was to evaluate the Chagas/Bio-Manguinhos Lateral Flow Immunochromatographic Rapid Test (Chagas-LFRT) for the diagnosis of T. cruzi infection in domestic dogs and the potential of application of this diagnostic platform to wild canid species. Two recombinant proteins (IBMP-8.1 and IBMP-8.4) that displayed the best performance in the enzyme immunoassay (ELISA) in previous studies were tested in a platform with two diagnostic bands. A panel of 281 dog serum samples was evaluated: 133 positive for T. cruzi by serological diagnosis, including 20 samples with positive blood cultures belonging to different discrete typing units (DTUs); 129 negative samples; and 19 samples from dogs infected by other trypanosomatids: Leishmania infantum , Trypanosoma rangeli , Trypanosoma caninum and Crithidia mellificae , in addition to samples infected by Anaplasma platys , Dirofilaria immitis and Erlichia sp. that were employed to evaluate eventual cross-reactions. We also evaluated the Chagas-LFRT to detect T. cruzi infection in 9 serum samples from six wild canid species. We observed that the intensity pattern of the bands was directly proportional to the serological titer observed in IFAT. The sensitivity was 94%, the specificity was 91% according to the ROC curve, and the defined cutoff was an optical density of 4.8. The agreement obtained was considered substantial by the kappa analysis (84%). From T. cruzi positive hemoculture samples, 88.9% were positive by ... |
author2 |
Conselho Nacional de Desenvolvimento Científico e Tecnológico Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro |
format |
Article in Journal/Newspaper |
author |
Rodrigues, Esthefany S. Santos, Gilbert Q. Silva, Marlon Vicente da Barros, Juliana H. S. Bernardo, Aline R. Diniz, Rafaela L. Rubim, Nara M. Roque, André L. R. Jansen, Ana Maria Silva, Edimilson D. Xavier, Samanta C. C. |
author_facet |
Rodrigues, Esthefany S. Santos, Gilbert Q. Silva, Marlon Vicente da Barros, Juliana H. S. Bernardo, Aline R. Diniz, Rafaela L. Rubim, Nara M. Roque, André L. R. Jansen, Ana Maria Silva, Edimilson D. Xavier, Samanta C. C. |
author_sort |
Rodrigues, Esthefany S. |
title |
Chagas Immunochromatographic Rapid Test in the Serological Diagnosis of Trypanosoma cruzi Infection in Wild and Domestic Canids |
title_short |
Chagas Immunochromatographic Rapid Test in the Serological Diagnosis of Trypanosoma cruzi Infection in Wild and Domestic Canids |
title_full |
Chagas Immunochromatographic Rapid Test in the Serological Diagnosis of Trypanosoma cruzi Infection in Wild and Domestic Canids |
title_fullStr |
Chagas Immunochromatographic Rapid Test in the Serological Diagnosis of Trypanosoma cruzi Infection in Wild and Domestic Canids |
title_full_unstemmed |
Chagas Immunochromatographic Rapid Test in the Serological Diagnosis of Trypanosoma cruzi Infection in Wild and Domestic Canids |
title_sort |
chagas immunochromatographic rapid test in the serological diagnosis of trypanosoma cruzi infection in wild and domestic canids |
publisher |
Frontiers Media SA |
publishDate |
2022 |
url |
http://dx.doi.org/10.3389/fcimb.2022.835383 https://www.frontiersin.org/articles/10.3389/fcimb.2022.835383/full |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Frontiers in Cellular and Infection Microbiology volume 12 ISSN 2235-2988 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fcimb.2022.835383 |
container_title |
Frontiers in Cellular and Infection Microbiology |
container_volume |
12 |
_version_ |
1797581048496783360 |