Bioinformatic and Mechanistic Analysis of the Palmerolide PKS-NRPS Biosynthetic Pathway From the Microbiome of an Antarctic Ascidian
Complex interactions exist between microbiomes and their hosts. Increasingly, defensive metabolites that have been attributed to host biosynthetic capability are now being recognized as products of host-associated microbes. These unique metabolites often have bioactivity targets in human disease and...
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Online Access: | http://dx.doi.org/10.3389/fchem.2021.802574 https://www.frontiersin.org/articles/10.3389/fchem.2021.802574/full |
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crfrontiers:10.3389/fchem.2021.802574 2024-06-23T07:47:00+00:00 Bioinformatic and Mechanistic Analysis of the Palmerolide PKS-NRPS Biosynthetic Pathway From the Microbiome of an Antarctic Ascidian Avalon, Nicole E. Murray, Alison E. Daligault, Hajnalka E. Lo, Chien-Chi Davenport, Karen W. Dichosa, Armand E. K. Chain, Patrick S. G. Baker, Bill J. National Institutes of Health National Science Foundation 2021 http://dx.doi.org/10.3389/fchem.2021.802574 https://www.frontiersin.org/articles/10.3389/fchem.2021.802574/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Chemistry volume 9 ISSN 2296-2646 journal-article 2021 crfrontiers https://doi.org/10.3389/fchem.2021.802574 2024-06-04T05:52:34Z Complex interactions exist between microbiomes and their hosts. Increasingly, defensive metabolites that have been attributed to host biosynthetic capability are now being recognized as products of host-associated microbes. These unique metabolites often have bioactivity targets in human disease and can be purposed as pharmaceuticals. Polyketides are a complex family of natural products that often serve as defensive metabolites for competitive or pro-survival purposes for the producing organism, while demonstrating bioactivity in human diseases as cholesterol lowering agents, anti-infectives, and anti-tumor agents. Marine invertebrates and microbes are a rich source of polyketides. Palmerolide A, a polyketide isolated from the Antarctic ascidian Synoicum adareanum, is a vacuolar-ATPase inhibitor with potent bioactivity against melanoma cell lines. The biosynthetic gene clusters (BGCs) responsible for production of secondary metabolites are encoded in the genomes of the producers as discrete genomic elements. A candidate palmerolide BGC was identified from a S. adareanum microbiome-metagenome based on a high degree of congruence with a chemical structure-based retrobiosynthetic prediction. Protein family homology analysis, conserved domain searches, active site and motif identification were used to identify and propose the function of the ∼75 kbp trans -acyltransferase (AT) polyketide synthase-non-ribosomal synthase (PKS-NRPS) domains responsible for the stepwise synthesis of palmerolide A. Though PKS systems often act in a predictable co-linear sequence, this BGC includes multiple trans -acting enzymatic domains, a non-canonical condensation termination domain, a bacterial luciferase-like monooxygenase (LLM), and is found in multiple copies within the metagenome-assembled genome (MAG). Detailed inspection of the five highly similar pal BGC copies suggests the potential for biosynthesis of other members of the palmerolide chemical family. This is the first delineation of a biosynthetic gene cluster from an ... Article in Journal/Newspaper Antarc* Antarctic Frontiers (Publisher) Antarctic The Antarctic Frontiers in Chemistry 9 |
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Frontiers (Publisher) |
op_collection_id |
crfrontiers |
language |
unknown |
description |
Complex interactions exist between microbiomes and their hosts. Increasingly, defensive metabolites that have been attributed to host biosynthetic capability are now being recognized as products of host-associated microbes. These unique metabolites often have bioactivity targets in human disease and can be purposed as pharmaceuticals. Polyketides are a complex family of natural products that often serve as defensive metabolites for competitive or pro-survival purposes for the producing organism, while demonstrating bioactivity in human diseases as cholesterol lowering agents, anti-infectives, and anti-tumor agents. Marine invertebrates and microbes are a rich source of polyketides. Palmerolide A, a polyketide isolated from the Antarctic ascidian Synoicum adareanum, is a vacuolar-ATPase inhibitor with potent bioactivity against melanoma cell lines. The biosynthetic gene clusters (BGCs) responsible for production of secondary metabolites are encoded in the genomes of the producers as discrete genomic elements. A candidate palmerolide BGC was identified from a S. adareanum microbiome-metagenome based on a high degree of congruence with a chemical structure-based retrobiosynthetic prediction. Protein family homology analysis, conserved domain searches, active site and motif identification were used to identify and propose the function of the ∼75 kbp trans -acyltransferase (AT) polyketide synthase-non-ribosomal synthase (PKS-NRPS) domains responsible for the stepwise synthesis of palmerolide A. Though PKS systems often act in a predictable co-linear sequence, this BGC includes multiple trans -acting enzymatic domains, a non-canonical condensation termination domain, a bacterial luciferase-like monooxygenase (LLM), and is found in multiple copies within the metagenome-assembled genome (MAG). Detailed inspection of the five highly similar pal BGC copies suggests the potential for biosynthesis of other members of the palmerolide chemical family. This is the first delineation of a biosynthetic gene cluster from an ... |
author2 |
National Institutes of Health National Science Foundation |
format |
Article in Journal/Newspaper |
author |
Avalon, Nicole E. Murray, Alison E. Daligault, Hajnalka E. Lo, Chien-Chi Davenport, Karen W. Dichosa, Armand E. K. Chain, Patrick S. G. Baker, Bill J. |
spellingShingle |
Avalon, Nicole E. Murray, Alison E. Daligault, Hajnalka E. Lo, Chien-Chi Davenport, Karen W. Dichosa, Armand E. K. Chain, Patrick S. G. Baker, Bill J. Bioinformatic and Mechanistic Analysis of the Palmerolide PKS-NRPS Biosynthetic Pathway From the Microbiome of an Antarctic Ascidian |
author_facet |
Avalon, Nicole E. Murray, Alison E. Daligault, Hajnalka E. Lo, Chien-Chi Davenport, Karen W. Dichosa, Armand E. K. Chain, Patrick S. G. Baker, Bill J. |
author_sort |
Avalon, Nicole E. |
title |
Bioinformatic and Mechanistic Analysis of the Palmerolide PKS-NRPS Biosynthetic Pathway From the Microbiome of an Antarctic Ascidian |
title_short |
Bioinformatic and Mechanistic Analysis of the Palmerolide PKS-NRPS Biosynthetic Pathway From the Microbiome of an Antarctic Ascidian |
title_full |
Bioinformatic and Mechanistic Analysis of the Palmerolide PKS-NRPS Biosynthetic Pathway From the Microbiome of an Antarctic Ascidian |
title_fullStr |
Bioinformatic and Mechanistic Analysis of the Palmerolide PKS-NRPS Biosynthetic Pathway From the Microbiome of an Antarctic Ascidian |
title_full_unstemmed |
Bioinformatic and Mechanistic Analysis of the Palmerolide PKS-NRPS Biosynthetic Pathway From the Microbiome of an Antarctic Ascidian |
title_sort |
bioinformatic and mechanistic analysis of the palmerolide pks-nrps biosynthetic pathway from the microbiome of an antarctic ascidian |
publisher |
Frontiers Media SA |
publishDate |
2021 |
url |
http://dx.doi.org/10.3389/fchem.2021.802574 https://www.frontiersin.org/articles/10.3389/fchem.2021.802574/full |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Frontiers in Chemistry volume 9 ISSN 2296-2646 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fchem.2021.802574 |
container_title |
Frontiers in Chemistry |
container_volume |
9 |
_version_ |
1802650048639533056 |