Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals
Major histocompatibility complex (MHC) class I and II are crucial for the adaptive immune system because they are involved in peptide presentation to T cells. Until recently, it was believed that MHC genes and their associated immune components had been conserved since their evolutionary emergence i...
Published in: | Frontiers in Cell and Developmental Biology |
---|---|
Main Authors: | , , , , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Frontiers Media SA
2023
|
Subjects: | |
Online Access: | http://dx.doi.org/10.3389/fcell.2023.1050323 https://www.frontiersin.org/articles/10.3389/fcell.2023.1050323/full |
id |
crfrontiers:10.3389/fcell.2023.1050323 |
---|---|
record_format |
openpolar |
spelling |
crfrontiers:10.3389/fcell.2023.1050323 2024-05-19T07:37:02+00:00 Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals Bjørnestad, Synne Arstad Solbakken, Monica Hongrø Jakobsen, Kjetill S. Jentoft, Sissel Bakke, Oddmund Progida, Cinzia Universitetet i Oslo 2023 http://dx.doi.org/10.3389/fcell.2023.1050323 https://www.frontiersin.org/articles/10.3389/fcell.2023.1050323/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Cell and Developmental Biology volume 11 ISSN 2296-634X journal-article 2023 crfrontiers https://doi.org/10.3389/fcell.2023.1050323 2024-05-01T06:51:22Z Major histocompatibility complex (MHC) class I and II are crucial for the adaptive immune system because they are involved in peptide presentation to T cells. Until recently, it was believed that MHC genes and their associated immune components had been conserved since their evolutionary emergence in jawed fish. However, sequencing of the Atlantic cod ( Gadus morhua ) genome revealed a loss of MHC class II genes, and an extreme expansion of MHC class I genes. These findings lead to the hypothesis that a loss of the MHC class II pathway coincided with a more versatile use of MHC class I, but so far there is no direct experimental evidence in support of this. To gain a deeper understanding of the function of the expanded MHC class I, we selected five MHC class I gene variants representing five of the six clades identified in previous studies and investigated their intracellular localization in human and Atlantic cod larval cells. Intriguingly, we uncovered that all selected MHC class I variants localize to endolysosomal compartments in Atlantic cod cells. Additionally, by introducing point mutations or deletions in the cytosolic tail, we found that hypothetical sorting signals in the MHC class I cytosolic tail do not influence MHC class I trafficking. Moreover, we demonstrated that in Atlantic cod, tapasin and MHC class I colocalize on endolysosomes suggesting that peptide-loading assistance and stabilization of MHC class I occurs outside the endoplasmic reticulum. Altogether, our results demonstrate that MHC class I from Atlantic cod is sorted to the endolysosomal system, which may indicate that it interacts with exogenous peptides for potential cross presentation. Article in Journal/Newspaper atlantic cod Gadus morhua Frontiers (Publisher) Frontiers in Cell and Developmental Biology 11 |
institution |
Open Polar |
collection |
Frontiers (Publisher) |
op_collection_id |
crfrontiers |
language |
unknown |
description |
Major histocompatibility complex (MHC) class I and II are crucial for the adaptive immune system because they are involved in peptide presentation to T cells. Until recently, it was believed that MHC genes and their associated immune components had been conserved since their evolutionary emergence in jawed fish. However, sequencing of the Atlantic cod ( Gadus morhua ) genome revealed a loss of MHC class II genes, and an extreme expansion of MHC class I genes. These findings lead to the hypothesis that a loss of the MHC class II pathway coincided with a more versatile use of MHC class I, but so far there is no direct experimental evidence in support of this. To gain a deeper understanding of the function of the expanded MHC class I, we selected five MHC class I gene variants representing five of the six clades identified in previous studies and investigated their intracellular localization in human and Atlantic cod larval cells. Intriguingly, we uncovered that all selected MHC class I variants localize to endolysosomal compartments in Atlantic cod cells. Additionally, by introducing point mutations or deletions in the cytosolic tail, we found that hypothetical sorting signals in the MHC class I cytosolic tail do not influence MHC class I trafficking. Moreover, we demonstrated that in Atlantic cod, tapasin and MHC class I colocalize on endolysosomes suggesting that peptide-loading assistance and stabilization of MHC class I occurs outside the endoplasmic reticulum. Altogether, our results demonstrate that MHC class I from Atlantic cod is sorted to the endolysosomal system, which may indicate that it interacts with exogenous peptides for potential cross presentation. |
author2 |
Universitetet i Oslo |
format |
Article in Journal/Newspaper |
author |
Bjørnestad, Synne Arstad Solbakken, Monica Hongrø Jakobsen, Kjetill S. Jentoft, Sissel Bakke, Oddmund Progida, Cinzia |
spellingShingle |
Bjørnestad, Synne Arstad Solbakken, Monica Hongrø Jakobsen, Kjetill S. Jentoft, Sissel Bakke, Oddmund Progida, Cinzia Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals |
author_facet |
Bjørnestad, Synne Arstad Solbakken, Monica Hongrø Jakobsen, Kjetill S. Jentoft, Sissel Bakke, Oddmund Progida, Cinzia |
author_sort |
Bjørnestad, Synne Arstad |
title |
Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals |
title_short |
Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals |
title_full |
Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals |
title_fullStr |
Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals |
title_full_unstemmed |
Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals |
title_sort |
atlantic cod (gadus morhua) mhc i localizes to endolysosomal compartments independently of cytosolic sorting signals |
publisher |
Frontiers Media SA |
publishDate |
2023 |
url |
http://dx.doi.org/10.3389/fcell.2023.1050323 https://www.frontiersin.org/articles/10.3389/fcell.2023.1050323/full |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Frontiers in Cell and Developmental Biology volume 11 ISSN 2296-634X |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fcell.2023.1050323 |
container_title |
Frontiers in Cell and Developmental Biology |
container_volume |
11 |
_version_ |
1799476280469487616 |