LC3-Mediated Mitophagy After CCCP or Vibrio splendidus Exposure in the Pacific Oyster Crassostrea gigas
Mitochondrial selective autophagy, known as mitophagy, surveils the mitochondrial population by eliminating superfluous and/or impaired organelles to mediate cellular survival and viability in response to injury/trauma and infection. In this study, the components of the mitophagy pathway in the Paci...
Published in: | Frontiers in Cell and Developmental Biology |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Frontiers Media SA
2022
|
Subjects: | |
Online Access: | http://dx.doi.org/10.3389/fcell.2022.885478 https://www.frontiersin.org/articles/10.3389/fcell.2022.885478/full |
id |
crfrontiers:10.3389/fcell.2022.885478 |
---|---|
record_format |
openpolar |
spelling |
crfrontiers:10.3389/fcell.2022.885478 2024-05-19T07:39:16+00:00 LC3-Mediated Mitophagy After CCCP or Vibrio splendidus Exposure in the Pacific Oyster Crassostrea gigas Sun, Jiejie Lv, Xiaoqian Leng, Jinyuan Wang, Lingling Song, Linsheng 2022 http://dx.doi.org/10.3389/fcell.2022.885478 https://www.frontiersin.org/articles/10.3389/fcell.2022.885478/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Cell and Developmental Biology volume 10 ISSN 2296-634X journal-article 2022 crfrontiers https://doi.org/10.3389/fcell.2022.885478 2024-05-01T06:48:58Z Mitochondrial selective autophagy, known as mitophagy, surveils the mitochondrial population by eliminating superfluous and/or impaired organelles to mediate cellular survival and viability in response to injury/trauma and infection. In this study, the components of the mitophagy pathway in the Pacific oyster Crassostrea gigas were screened from NCBI with reference to the protein sequences of the human mitophagy process. A total of 10 mitophagy process–related genes were identified from C. gigas , including NIX, FUNDC1, PHB2, Cardiolipin, P62, VDAC2, MFN2, PARL, MPP, and OPTN. They shared high similarities with their homologs in the human mitophagy pathway and were expressed in various tissues of C. gigas . After CCCP exposure, the fluorescence intensity of the mitochondrial probe JC-1 monomers increased significantly in hemocytes, while the fluorescence intensity of JC-1 aggregates decreased significantly. Meanwhile, the fluorescence of lysosomes was found to be co-localized with that of Cg LC3 and mitochondria in CCCP-treated hemocytes. Double- and single-membrane-bound vacuoles resembling autophagic structures were observed in the hemocytes after CCCP exposure. The fluorescence intensity of JC-1 monomers and the abundance of Cg LC3Ⅱ in hemocytes both increased after Vibrio splendidus exposure. At the same time, the green signals of Cg LC3 were co-localized with red signals of the mitochondria, and the fluorescence intensity of autophagy increased significantly in hemocytes after V. splendidus exposure. The results confirmed the existence of a complete mitophagy pathway in mollusks for the first time, which was helpful for further study on the function of mitochondrial autophagy in mollusks. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Frontiers (Publisher) Frontiers in Cell and Developmental Biology 10 |
institution |
Open Polar |
collection |
Frontiers (Publisher) |
op_collection_id |
crfrontiers |
language |
unknown |
description |
Mitochondrial selective autophagy, known as mitophagy, surveils the mitochondrial population by eliminating superfluous and/or impaired organelles to mediate cellular survival and viability in response to injury/trauma and infection. In this study, the components of the mitophagy pathway in the Pacific oyster Crassostrea gigas were screened from NCBI with reference to the protein sequences of the human mitophagy process. A total of 10 mitophagy process–related genes were identified from C. gigas , including NIX, FUNDC1, PHB2, Cardiolipin, P62, VDAC2, MFN2, PARL, MPP, and OPTN. They shared high similarities with their homologs in the human mitophagy pathway and were expressed in various tissues of C. gigas . After CCCP exposure, the fluorescence intensity of the mitochondrial probe JC-1 monomers increased significantly in hemocytes, while the fluorescence intensity of JC-1 aggregates decreased significantly. Meanwhile, the fluorescence of lysosomes was found to be co-localized with that of Cg LC3 and mitochondria in CCCP-treated hemocytes. Double- and single-membrane-bound vacuoles resembling autophagic structures were observed in the hemocytes after CCCP exposure. The fluorescence intensity of JC-1 monomers and the abundance of Cg LC3Ⅱ in hemocytes both increased after Vibrio splendidus exposure. At the same time, the green signals of Cg LC3 were co-localized with red signals of the mitochondria, and the fluorescence intensity of autophagy increased significantly in hemocytes after V. splendidus exposure. The results confirmed the existence of a complete mitophagy pathway in mollusks for the first time, which was helpful for further study on the function of mitochondrial autophagy in mollusks. |
format |
Article in Journal/Newspaper |
author |
Sun, Jiejie Lv, Xiaoqian Leng, Jinyuan Wang, Lingling Song, Linsheng |
spellingShingle |
Sun, Jiejie Lv, Xiaoqian Leng, Jinyuan Wang, Lingling Song, Linsheng LC3-Mediated Mitophagy After CCCP or Vibrio splendidus Exposure in the Pacific Oyster Crassostrea gigas |
author_facet |
Sun, Jiejie Lv, Xiaoqian Leng, Jinyuan Wang, Lingling Song, Linsheng |
author_sort |
Sun, Jiejie |
title |
LC3-Mediated Mitophagy After CCCP or Vibrio splendidus Exposure in the Pacific Oyster Crassostrea gigas |
title_short |
LC3-Mediated Mitophagy After CCCP or Vibrio splendidus Exposure in the Pacific Oyster Crassostrea gigas |
title_full |
LC3-Mediated Mitophagy After CCCP or Vibrio splendidus Exposure in the Pacific Oyster Crassostrea gigas |
title_fullStr |
LC3-Mediated Mitophagy After CCCP or Vibrio splendidus Exposure in the Pacific Oyster Crassostrea gigas |
title_full_unstemmed |
LC3-Mediated Mitophagy After CCCP or Vibrio splendidus Exposure in the Pacific Oyster Crassostrea gigas |
title_sort |
lc3-mediated mitophagy after cccp or vibrio splendidus exposure in the pacific oyster crassostrea gigas |
publisher |
Frontiers Media SA |
publishDate |
2022 |
url |
http://dx.doi.org/10.3389/fcell.2022.885478 https://www.frontiersin.org/articles/10.3389/fcell.2022.885478/full |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Frontiers in Cell and Developmental Biology volume 10 ISSN 2296-634X |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fcell.2022.885478 |
container_title |
Frontiers in Cell and Developmental Biology |
container_volume |
10 |
_version_ |
1799478827495194624 |