Expression patterns of the poplar NF-Y gene family in response to Alternaria alternata and hormone treatment and the role of PdbNF-YA11 in disease resistance
Plant nuclear factor-Y (NF-Y) transcription factors (TFs) are key regulators of growth and stress resistance. However, the role of NF-Y TFs in poplar in response to biotic stress is still unclear. In this study, we cloned 26 PdbNF-Y encoding genes in the hybrid poplar P. davidiana × P. bollena , inc...
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crfrontiers:10.3389/fbioe.2022.956271 2024-06-23T07:55:59+00:00 Expression patterns of the poplar NF-Y gene family in response to Alternaria alternata and hormone treatment and the role of PdbNF-YA11 in disease resistance Huang, Ying Ma, Huijun Wang, Xiaodong Cui, Tianxiang Han, Gang Zhang, Yu Wang, Chao National Natural Science Foundation of China 2022 http://dx.doi.org/10.3389/fbioe.2022.956271 https://www.frontiersin.org/articles/10.3389/fbioe.2022.956271/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Bioengineering and Biotechnology volume 10 ISSN 2296-4185 journal-article 2022 crfrontiers https://doi.org/10.3389/fbioe.2022.956271 2024-06-11T04:09:32Z Plant nuclear factor-Y (NF-Y) transcription factors (TFs) are key regulators of growth and stress resistance. However, the role of NF-Y TFs in poplar in response to biotic stress is still unclear. In this study, we cloned 26 PdbNF-Y encoding genes in the hybrid poplar P. davidiana × P. bollena , including 12 PdbNF-YA s, six PdbNF-YB s, and eight PdbNF-YC s. Their physical and chemical parameters, conserved domains, and phylogeny were subsequently analyzed. The protein–protein interaction (PPI) network showed that the three PdbNF-Y subunits may interact with NF-Y proteins belonging to two other subfamilies and other TFs. Tissue expression analysis revealed that PdbNF-Y s exhibited three distinct expression patterns in three tissues. Cis-elements related to stress-responsiveness were found in the promoters of PdbNF-Y s, and most PdbNF-Y s were shown to be differentially expressed under Alternaria alternata and hormone treatments. Compared with the PdbNF-YB and PdbNF-YC subfamilies, more PdbNF-YA s were significantly induced under the two treatments. Moreover, loss- and gain-of-function analyses showed that PdbNF-YA11 plays a positive role in poplar resistance to A. alternata . Additionally, RT‒qPCR analyses showed that overexpression and silencing PdbNF-YA11 altered the transcript levels of JA-related genes, including LOX , AOS , AOC , COI , JAZ , ORCA , and MYC , suggesting that PdbNF-YA11 -mediated disease resistance is related to activation of the JA pathway. Our findings will contribute to functional analysis of NF-Y genes in woody plants, especially their roles in response to biotic stress. Article in Journal/Newspaper Orca Frontiers (Publisher) Frontiers in Bioengineering and Biotechnology 10 |
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Plant nuclear factor-Y (NF-Y) transcription factors (TFs) are key regulators of growth and stress resistance. However, the role of NF-Y TFs in poplar in response to biotic stress is still unclear. In this study, we cloned 26 PdbNF-Y encoding genes in the hybrid poplar P. davidiana × P. bollena , including 12 PdbNF-YA s, six PdbNF-YB s, and eight PdbNF-YC s. Their physical and chemical parameters, conserved domains, and phylogeny were subsequently analyzed. The protein–protein interaction (PPI) network showed that the three PdbNF-Y subunits may interact with NF-Y proteins belonging to two other subfamilies and other TFs. Tissue expression analysis revealed that PdbNF-Y s exhibited three distinct expression patterns in three tissues. Cis-elements related to stress-responsiveness were found in the promoters of PdbNF-Y s, and most PdbNF-Y s were shown to be differentially expressed under Alternaria alternata and hormone treatments. Compared with the PdbNF-YB and PdbNF-YC subfamilies, more PdbNF-YA s were significantly induced under the two treatments. Moreover, loss- and gain-of-function analyses showed that PdbNF-YA11 plays a positive role in poplar resistance to A. alternata . Additionally, RT‒qPCR analyses showed that overexpression and silencing PdbNF-YA11 altered the transcript levels of JA-related genes, including LOX , AOS , AOC , COI , JAZ , ORCA , and MYC , suggesting that PdbNF-YA11 -mediated disease resistance is related to activation of the JA pathway. Our findings will contribute to functional analysis of NF-Y genes in woody plants, especially their roles in response to biotic stress. |
author2 |
National Natural Science Foundation of China |
format |
Article in Journal/Newspaper |
author |
Huang, Ying Ma, Huijun Wang, Xiaodong Cui, Tianxiang Han, Gang Zhang, Yu Wang, Chao |
spellingShingle |
Huang, Ying Ma, Huijun Wang, Xiaodong Cui, Tianxiang Han, Gang Zhang, Yu Wang, Chao Expression patterns of the poplar NF-Y gene family in response to Alternaria alternata and hormone treatment and the role of PdbNF-YA11 in disease resistance |
author_facet |
Huang, Ying Ma, Huijun Wang, Xiaodong Cui, Tianxiang Han, Gang Zhang, Yu Wang, Chao |
author_sort |
Huang, Ying |
title |
Expression patterns of the poplar NF-Y gene family in response to Alternaria alternata and hormone treatment and the role of PdbNF-YA11 in disease resistance |
title_short |
Expression patterns of the poplar NF-Y gene family in response to Alternaria alternata and hormone treatment and the role of PdbNF-YA11 in disease resistance |
title_full |
Expression patterns of the poplar NF-Y gene family in response to Alternaria alternata and hormone treatment and the role of PdbNF-YA11 in disease resistance |
title_fullStr |
Expression patterns of the poplar NF-Y gene family in response to Alternaria alternata and hormone treatment and the role of PdbNF-YA11 in disease resistance |
title_full_unstemmed |
Expression patterns of the poplar NF-Y gene family in response to Alternaria alternata and hormone treatment and the role of PdbNF-YA11 in disease resistance |
title_sort |
expression patterns of the poplar nf-y gene family in response to alternaria alternata and hormone treatment and the role of pdbnf-ya11 in disease resistance |
publisher |
Frontiers Media SA |
publishDate |
2022 |
url |
http://dx.doi.org/10.3389/fbioe.2022.956271 https://www.frontiersin.org/articles/10.3389/fbioe.2022.956271/full |
genre |
Orca |
genre_facet |
Orca |
op_source |
Frontiers in Bioengineering and Biotechnology volume 10 ISSN 2296-4185 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fbioe.2022.956271 |
container_title |
Frontiers in Bioengineering and Biotechnology |
container_volume |
10 |
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1802648809691414528 |