Genetic diversity of F1 and F2 interspecific hybrids between dwarf birch (Betula nana L.) and Himalayan birch (B. utilis var. jacquemontii (Spach) Winkl. 'Doorenbos') using RAPD-PCR markers and ploidy analysis.
Crosses between Betula nana and B. utilis 'Doorenbos' were undertaken in order to obtain interspecific hybrids which could be characterized by wide spreading stems, strong branching habit, decorative clear white bark and an interesting shape of purple leaves. The research purpose was to ex...
Published in: | Acta Biochimica Polonica |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Frontiers Media SA
2014
|
Subjects: | |
Online Access: | http://dx.doi.org/10.18388/abp.2014_1885 https://ojs.ptbioch.edu.pl/index.php/abp/article/download/1885/518 |
id |
crfrontiers:10.18388/abp.2014_1885 |
---|---|
record_format |
openpolar |
spelling |
crfrontiers:10.18388/abp.2014_1885 2024-06-23T07:51:47+00:00 Genetic diversity of F1 and F2 interspecific hybrids between dwarf birch (Betula nana L.) and Himalayan birch (B. utilis var. jacquemontii (Spach) Winkl. 'Doorenbos') using RAPD-PCR markers and ploidy analysis. Czernicka, Małgorzata Pławiak, Jarosław Muras, Piotr 2014 http://dx.doi.org/10.18388/abp.2014_1885 https://ojs.ptbioch.edu.pl/index.php/abp/article/download/1885/518 unknown Frontiers Media SA Acta Biochimica Polonica volume 61, issue 2 ISSN 1734-154X 0001-527X journal-article 2014 crfrontiers https://doi.org/10.18388/abp.2014_1885 2024-06-04T05:51:59Z Crosses between Betula nana and B. utilis 'Doorenbos' were undertaken in order to obtain interspecific hybrids which could be characterized by wide spreading stems, strong branching habit, decorative clear white bark and an interesting shape of purple leaves. The research purpose was to examine genetic diversity of the 16 F1 and F2 putative progenies by using the RAPD-PCR method and the ploidy analysis. A total of 242 RAPD markers were scored with 24 primers and 220 (90.9%) polymorphic bands were found. In the NJ dendrogram, cluster I consisted of the female parent--B. nana and 12 hybrids and cluster II grouped the male parent--B. utilis 'Doorenbos' with 4 hybrids (F2/2, F1/8, F1/7 and F2/1). The 2-D scaling by PCoA was in agreement with the similarity index, i.e. two hybrids (F1/8, F2/2) grouped with the male parent while others with female parent. Classification of the hybrid plants by chromosome counting demonstrated that 13 hybrids were confirmed with accurate chromosome counts as being diploid (2n=2x=28) and 3 plants (F1/7, F1/8, F2/2) as triploid with 42 chromosomes. Article in Journal/Newspaper Betula nana Dwarf birch Frontiers (Publisher) Acta Biochimica Polonica 61 2 |
institution |
Open Polar |
collection |
Frontiers (Publisher) |
op_collection_id |
crfrontiers |
language |
unknown |
description |
Crosses between Betula nana and B. utilis 'Doorenbos' were undertaken in order to obtain interspecific hybrids which could be characterized by wide spreading stems, strong branching habit, decorative clear white bark and an interesting shape of purple leaves. The research purpose was to examine genetic diversity of the 16 F1 and F2 putative progenies by using the RAPD-PCR method and the ploidy analysis. A total of 242 RAPD markers were scored with 24 primers and 220 (90.9%) polymorphic bands were found. In the NJ dendrogram, cluster I consisted of the female parent--B. nana and 12 hybrids and cluster II grouped the male parent--B. utilis 'Doorenbos' with 4 hybrids (F2/2, F1/8, F1/7 and F2/1). The 2-D scaling by PCoA was in agreement with the similarity index, i.e. two hybrids (F1/8, F2/2) grouped with the male parent while others with female parent. Classification of the hybrid plants by chromosome counting demonstrated that 13 hybrids were confirmed with accurate chromosome counts as being diploid (2n=2x=28) and 3 plants (F1/7, F1/8, F2/2) as triploid with 42 chromosomes. |
format |
Article in Journal/Newspaper |
author |
Czernicka, Małgorzata Pławiak, Jarosław Muras, Piotr |
spellingShingle |
Czernicka, Małgorzata Pławiak, Jarosław Muras, Piotr Genetic diversity of F1 and F2 interspecific hybrids between dwarf birch (Betula nana L.) and Himalayan birch (B. utilis var. jacquemontii (Spach) Winkl. 'Doorenbos') using RAPD-PCR markers and ploidy analysis. |
author_facet |
Czernicka, Małgorzata Pławiak, Jarosław Muras, Piotr |
author_sort |
Czernicka, Małgorzata |
title |
Genetic diversity of F1 and F2 interspecific hybrids between dwarf birch (Betula nana L.) and Himalayan birch (B. utilis var. jacquemontii (Spach) Winkl. 'Doorenbos') using RAPD-PCR markers and ploidy analysis. |
title_short |
Genetic diversity of F1 and F2 interspecific hybrids between dwarf birch (Betula nana L.) and Himalayan birch (B. utilis var. jacquemontii (Spach) Winkl. 'Doorenbos') using RAPD-PCR markers and ploidy analysis. |
title_full |
Genetic diversity of F1 and F2 interspecific hybrids between dwarf birch (Betula nana L.) and Himalayan birch (B. utilis var. jacquemontii (Spach) Winkl. 'Doorenbos') using RAPD-PCR markers and ploidy analysis. |
title_fullStr |
Genetic diversity of F1 and F2 interspecific hybrids between dwarf birch (Betula nana L.) and Himalayan birch (B. utilis var. jacquemontii (Spach) Winkl. 'Doorenbos') using RAPD-PCR markers and ploidy analysis. |
title_full_unstemmed |
Genetic diversity of F1 and F2 interspecific hybrids between dwarf birch (Betula nana L.) and Himalayan birch (B. utilis var. jacquemontii (Spach) Winkl. 'Doorenbos') using RAPD-PCR markers and ploidy analysis. |
title_sort |
genetic diversity of f1 and f2 interspecific hybrids between dwarf birch (betula nana l.) and himalayan birch (b. utilis var. jacquemontii (spach) winkl. 'doorenbos') using rapd-pcr markers and ploidy analysis. |
publisher |
Frontiers Media SA |
publishDate |
2014 |
url |
http://dx.doi.org/10.18388/abp.2014_1885 https://ojs.ptbioch.edu.pl/index.php/abp/article/download/1885/518 |
genre |
Betula nana Dwarf birch |
genre_facet |
Betula nana Dwarf birch |
op_source |
Acta Biochimica Polonica volume 61, issue 2 ISSN 1734-154X 0001-527X |
op_doi |
https://doi.org/10.18388/abp.2014_1885 |
container_title |
Acta Biochimica Polonica |
container_volume |
61 |
container_issue |
2 |
_version_ |
1802642921558638592 |