Discovery and characterization of Hv1-type proton channels in reef-building corals
Voltage-dependent proton-permeable channels are membrane proteins mediating a number of important physiological functions. Here we report the presence of a gene encoding H v 1 voltage-dependent, proton-permeable channels in two species of reef-building corals. We performed a characterization of thei...
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2021
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Online Access: | http://dx.doi.org/10.7554/elife.69248 https://cdn.elifesciences.org/articles/69248/elife-69248-v1.pdf https://cdn.elifesciences.org/articles/69248/elife-69248-v1.xml https://elifesciences.org/articles/69248 |
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crelifesciences:10.7554/elife.69248 2024-05-19T07:46:35+00:00 Discovery and characterization of Hv1-type proton channels in reef-building corals Rangel-Yescas, Gisela Cervantes, Cecilia Cervantes-Rocha, Miguel A Suárez-Delgado, Esteban Banaszak, Anastazia T Maldonado, Ernesto Ramsey, Ian Scott Rosenbaum, Tamara Islas, Leon D Universidad Nacional Autónoma de México Universidad Nacional Autónoma de México Universidad Nacional Autónoma de México Consejo Nacional de Ciencia y Tecnología 2021 http://dx.doi.org/10.7554/elife.69248 https://cdn.elifesciences.org/articles/69248/elife-69248-v1.pdf https://cdn.elifesciences.org/articles/69248/elife-69248-v1.xml https://elifesciences.org/articles/69248 en eng eLife Sciences Publications, Ltd http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ eLife volume 10 ISSN 2050-084X journal-article 2021 crelifesciences https://doi.org/10.7554/elife.69248 2024-05-01T07:20:51Z Voltage-dependent proton-permeable channels are membrane proteins mediating a number of important physiological functions. Here we report the presence of a gene encoding H v 1 voltage-dependent, proton-permeable channels in two species of reef-building corals. We performed a characterization of their biophysical properties and found that these channels are fast-activating and modulated by the pH gradient in a distinct manner. The biophysical properties of these novel channels make them interesting model systems. We have also developed an allosteric gating model that provides mechanistic insight into the modulation of voltage-dependence by protons. This work also represents the first functional characterization of any ion channel in scleractinian corals. We discuss the implications of the presence of these channels in the membranes of coral cells in the calcification and pH-regulation processes and possible consequences of ocean acidification related to the function of these channels. Article in Journal/Newspaper Ocean acidification eLife eLife 10 |
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Open Polar |
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eLife |
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crelifesciences |
language |
English |
description |
Voltage-dependent proton-permeable channels are membrane proteins mediating a number of important physiological functions. Here we report the presence of a gene encoding H v 1 voltage-dependent, proton-permeable channels in two species of reef-building corals. We performed a characterization of their biophysical properties and found that these channels are fast-activating and modulated by the pH gradient in a distinct manner. The biophysical properties of these novel channels make them interesting model systems. We have also developed an allosteric gating model that provides mechanistic insight into the modulation of voltage-dependence by protons. This work also represents the first functional characterization of any ion channel in scleractinian corals. We discuss the implications of the presence of these channels in the membranes of coral cells in the calcification and pH-regulation processes and possible consequences of ocean acidification related to the function of these channels. |
author2 |
Universidad Nacional Autónoma de México Universidad Nacional Autónoma de México Universidad Nacional Autónoma de México Consejo Nacional de Ciencia y Tecnología |
format |
Article in Journal/Newspaper |
author |
Rangel-Yescas, Gisela Cervantes, Cecilia Cervantes-Rocha, Miguel A Suárez-Delgado, Esteban Banaszak, Anastazia T Maldonado, Ernesto Ramsey, Ian Scott Rosenbaum, Tamara Islas, Leon D |
spellingShingle |
Rangel-Yescas, Gisela Cervantes, Cecilia Cervantes-Rocha, Miguel A Suárez-Delgado, Esteban Banaszak, Anastazia T Maldonado, Ernesto Ramsey, Ian Scott Rosenbaum, Tamara Islas, Leon D Discovery and characterization of Hv1-type proton channels in reef-building corals |
author_facet |
Rangel-Yescas, Gisela Cervantes, Cecilia Cervantes-Rocha, Miguel A Suárez-Delgado, Esteban Banaszak, Anastazia T Maldonado, Ernesto Ramsey, Ian Scott Rosenbaum, Tamara Islas, Leon D |
author_sort |
Rangel-Yescas, Gisela |
title |
Discovery and characterization of Hv1-type proton channels in reef-building corals |
title_short |
Discovery and characterization of Hv1-type proton channels in reef-building corals |
title_full |
Discovery and characterization of Hv1-type proton channels in reef-building corals |
title_fullStr |
Discovery and characterization of Hv1-type proton channels in reef-building corals |
title_full_unstemmed |
Discovery and characterization of Hv1-type proton channels in reef-building corals |
title_sort |
discovery and characterization of hv1-type proton channels in reef-building corals |
publisher |
eLife Sciences Publications, Ltd |
publishDate |
2021 |
url |
http://dx.doi.org/10.7554/elife.69248 https://cdn.elifesciences.org/articles/69248/elife-69248-v1.pdf https://cdn.elifesciences.org/articles/69248/elife-69248-v1.xml https://elifesciences.org/articles/69248 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
eLife volume 10 ISSN 2050-084X |
op_rights |
http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.7554/elife.69248 |
container_title |
eLife |
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10 |
_version_ |
1799486790483050496 |