| id |
crelifesciences:10.7554/elife.40497.004
|
| record_format |
openpolar
|
| spelling |
crelifesciences:10.7554/elife.40497.004 2023-07-23T04:21:16+02:00 Figure 1—figure supplement 2. Simplified Schematic of Lattice Light Sheet Microscope. The schematic is organized to show the major modules of the microscope. The Laser Combiner module contains six lasers (three shown here) for excitation ranging from 405 to 639 nm, each of which are independently expanded and collimated by using a pair of lenses that serve as a beam expander (BE). The paths of each laser are combined and made collinear by using 1 mirror and 1 dichroic mirror (DC) per laser. The combined beams are then input into an Acousto-optic tunable filter (AOTF) for rapid switching between lasers and control of power. A beam dump (BD) is used to safely capture the light from lasers not being used. To achieve constant and controllable photoactivation the laser combiner was modified to include a 405 nm laser that bypasses the AOTF. A half-wave plate (HWP) is used to adjust the polarization of the input light to the Spatial Patterning module. For spatial patterning, a pair of cylindrical lenses (CL) is used to stretch the Gaussian beam output from the Laser Combiner module into a thin stripe, which illuminates the Spatial Light Modulator (SLM) after passing through a polarizing beam splitter (PBS) and a second HWP. A lens projects the Fourier transform of the plane of the SLM onto an annular mask which is used to confine the spatial frequencies of the patterned light to the desired minimum and maximum numerical apertures. In the scanning module, a pair of lenses de-magnifies and projects the annular mask plane onto first the z-galvo scanning mirror for moving the light sheet through the sample, and a second pair-of lenses relays the plane of the z-galvo onto the x-galvo for dithering the sheet for uniform illumination. Another pair of lenses is then used to project a magnified image of the galvo planes to the back focal plane of the excitation objective (EO) which focuses the light to project the lattice pattern through the sample. An orthogonally placed detection objective (DO) collects the emission light, and a tube-lens (TL) then forms an image at each cameras sensor plane. A dichroic mirror first splits the light into red (>560 nm) and green (<560 nm) channels, followed by a narrower bandpass emission filter (EMF) for further filtering before each camera. With the exception of the modifications to the laser combiner module and the use of two Hamamatsu sCMOS ORCA Flash 4.0 cameras for detection the design is identical to what was originally described by Chen et al., 2014a and Chen et al., 2014b. 2018-12-27T13:00:24Z http://dx.doi.org/10.7554/elife.40497.004 unknown eLife Sciences Publications, Ltd component 2018 crelifesciences https://doi.org/10.7554/elife.40497.004 2023-07-05T08:40:01Z Other/Unknown Material Orca eLife (E-Journal - via Crossref) Stripe ENVELOPE(9.914,9.914,63.019,63.019)
|
| institution |
Open Polar
|
| collection |
eLife (E-Journal - via Crossref)
|
| op_collection_id |
crelifesciences
|
| language |
unknown
|
| format |
Other/Unknown Material
|
| title |
Figure 1—figure supplement 2. Simplified Schematic of Lattice Light Sheet Microscope.
|
| spellingShingle |
Figure 1—figure supplement 2. Simplified Schematic of Lattice Light Sheet Microscope.
|
| title_short |
Figure 1—figure supplement 2. Simplified Schematic of Lattice Light Sheet Microscope.
|
| title_full |
Figure 1—figure supplement 2. Simplified Schematic of Lattice Light Sheet Microscope.
|
| title_fullStr |
Figure 1—figure supplement 2. Simplified Schematic of Lattice Light Sheet Microscope.
|
| title_full_unstemmed |
Figure 1—figure supplement 2. Simplified Schematic of Lattice Light Sheet Microscope.
|
| title_sort |
figure 1—figure supplement 2. simplified schematic of lattice light sheet microscope.
|
| publisher |
eLife Sciences Publications, Ltd
|
| publishDate |
2018
|
| url |
http://dx.doi.org/10.7554/elife.40497.004
|
| long_lat |
ENVELOPE(9.914,9.914,63.019,63.019)
|
| geographic |
Stripe
|
| geographic_facet |
Stripe
|
| genre |
Orca
|
| genre_facet |
Orca
|
| op_doi |
https://doi.org/10.7554/elife.40497.004
|
| _version_ |
1772186625647837184
|