| id |
crelifesciences:10.7554/elife.14847.007
|
| record_format |
openpolar
|
| spelling |
crelifesciences:10.7554/elife.14847.007 2023-07-23T04:19:01+02:00 Figure 5. β-catenin from the cell membrane and AJ is required for the NOTCH-mediated activation of MYF5 expression. (A,B) Confocal stacks of somites after electroporation of WT β-catenin (A) or Y489F β-catenin (B). MYF5 expression is shown (in blue). (C) Bar charts showing the% of electroporated cells that were positive for MYF5 after electroporation of WT β-catenin (27.7%, in white) or Y489F β-catenin (6.4%, in black). (D) Schematic of the experimental design that was followed: (Myc-tagged) WT or a mutant Y489F β-catenin under a CAGGS ubiquitous promoter was electroporated in the DML together with the Tet-On Advanced transactivator (rtTA) and NICD inserted in the response vector. The β-catenin (WT or mutant) was expressed from the start of the experiment; NICD was induced 10 hr later. (E,F) Confocal stacks of somites after electroporation of (E) NICD (in green) and WT β-catenin (in red) or (F) NICD (in green) and Y489F β-catenin (in red). MYF5 expression is shown (in blue). (G) Bar charts showing the% of MYF5-positive cells after electroporation of NICD with WT β-catenin (88%, in white) or Y489F β-catenin (8.1%, in black). In each panel are indicated the antigens that were detected by immunostaining. DML: dorso-medial lip. Scale bars: 50 μm 2016-06-23T14:51:06Z http://dx.doi.org/10.7554/elife.14847.007 unknown eLife Sciences Publications, Ltd component 2016 crelifesciences https://doi.org/10.7554/elife.14847.007 2023-07-05T08:43:37Z Other/Unknown Material DML eLife (E-Journal - via Crossref)
|
| institution |
Open Polar
|
| collection |
eLife (E-Journal - via Crossref)
|
| op_collection_id |
crelifesciences
|
| language |
unknown
|
| format |
Other/Unknown Material
|
| title |
Figure 5. β-catenin from the cell membrane and AJ is required for the NOTCH-mediated activation of MYF5 expression.
|
| spellingShingle |
Figure 5. β-catenin from the cell membrane and AJ is required for the NOTCH-mediated activation of MYF5 expression.
|
| title_short |
Figure 5. β-catenin from the cell membrane and AJ is required for the NOTCH-mediated activation of MYF5 expression.
|
| title_full |
Figure 5. β-catenin from the cell membrane and AJ is required for the NOTCH-mediated activation of MYF5 expression.
|
| title_fullStr |
Figure 5. β-catenin from the cell membrane and AJ is required for the NOTCH-mediated activation of MYF5 expression.
|
| title_full_unstemmed |
Figure 5. β-catenin from the cell membrane and AJ is required for the NOTCH-mediated activation of MYF5 expression.
|
| title_sort |
figure 5. β-catenin from the cell membrane and aj is required for the notch-mediated activation of myf5 expression.
|
| publisher |
eLife Sciences Publications, Ltd
|
| publishDate |
2016
|
| url |
http://dx.doi.org/10.7554/elife.14847.007
|
| genre |
DML
|
| genre_facet |
DML
|
| op_doi |
https://doi.org/10.7554/elife.14847.007
|
| _version_ |
1772181771446648832
|