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crelifesciences:10.7554/elife.14847.005
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openpolar
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crelifesciences:10.7554/elife.14847.005 2023-07-23T04:19:01+02:00 Figure 4. Activation of TCF/β-catenin transcriptional activity and myogenesis by NOTCH is WNT-ligand independent. (A,B) Confocal stacks of somites, 6 hr after co-electroporation of 12Tf-d2EGFP (TOPflash reporter, in green), with H2B-RFP (in red) without (A) or with (B) DKK-1, and immunostained for MYF5 (in blue). (C–E) Bar charts showing (C) 33.3% of TOPflash-positive cells in the controls (in white) or 17.7% with DKK-1 (in black); (D) Bar charts indicating 56.8% of TOPflash-positive cells are MYF5-positive in control embryos, while 96.9% of TOPflash-positive cells are MYF5-positive when DKK1 is present; (E) percentage of electroporated cells that are MYF5-positive in controls: 25.7%; with DKK1: 26.6%. (F) Confocal stacks of somites after co-electroporation of HES1-DsRed-express-DR (NOTCH reporter, in red) or with 12Tf-d2EGFP (TOPflash reporter in green) and DKK1. (G) Bar charts showing the% of electroporated cells co-expressing the TOPflash and the NOTCH reporters when DKK1 is present (91.4%, in black) or absent (39.9%, in white). (H) Schematic representing the design of the double electroporation experiments, with a first electroporation targeting the neural tube (NT) and the migrating neural crest (in blue), followed by electroporation in the DML. (I–K) Confocal stacks of somites showing the expression of 12Tf-DsRed-express-DR (TOPflash reporter, in red) and the electroporation marker H2B-BFP (in blue) in the DML. Dorsal neural tube was electroporated with (I) GFP, (J) dominant-negative WNT1 (in green), or (K) wild-type WNT1 (in green). (L) Bar charts showing 19% of DN-WNT1-positive electroporated cells express the TOPflash reporter (in black), 67.6% of WT-WNT1-positive electroporated cells express the TOPflash reporter (in gray), controls (in white): 35.5%. (M) Bar charts showing 15.04% of DN-WNT1-positive electroporated cells express MYF5 (in black), 15.07% for WT-WNT1-positive electroporated cells (in gray), controls (in white): 15.08%. (N,O) Confocal stacks of somites showing the TOPflash reporter 12Tf-DsRed-express-DR (in red), the electroporation marker GFP (in green) and DKK1, electroporated into the DML. The dorsal neural tube was electroporated with (N) BFP or (O) dominant-negative DLL1 and BFP. (P) Bar charts showing the% of electroporated cells expressing the TOPflash reporter, DN-DLL1 + DKK1: 6%, in black; controls with DKK1: 22.3%, in white. (Q) Bar charts showing the% of electroporated cells expressing MYF5, DN-DLL1 + DKK1: 6.8% in black; controls with DKK1: 16.6%, in white. Dotted lines indicate the borders of the neural tube and somites. Abbreviations: NT: neural tube; DML: Dorso-Medial Lip; EP: electroporation. In each panel are indicated the antigens that were detected by immunostaining with the exception of native BFP blue fluorescence. Scale bars: 50 μm. 2016-06-23T18:51:06Z http://dx.doi.org/10.7554/elife.14847.005 unknown eLife Sciences Publications, Ltd component 2016 crelifesciences https://doi.org/10.7554/elife.14847.005 2023-07-05T08:43:44Z Other/Unknown Material DML eLife (E-Journal - via Crossref)
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Open Polar
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eLife (E-Journal - via Crossref)
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crelifesciences
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| language |
unknown
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Other/Unknown Material
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| title |
Figure 4. Activation of TCF/β-catenin transcriptional activity and myogenesis by NOTCH is WNT-ligand independent.
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| spellingShingle |
Figure 4. Activation of TCF/β-catenin transcriptional activity and myogenesis by NOTCH is WNT-ligand independent.
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| title_short |
Figure 4. Activation of TCF/β-catenin transcriptional activity and myogenesis by NOTCH is WNT-ligand independent.
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| title_full |
Figure 4. Activation of TCF/β-catenin transcriptional activity and myogenesis by NOTCH is WNT-ligand independent.
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| title_fullStr |
Figure 4. Activation of TCF/β-catenin transcriptional activity and myogenesis by NOTCH is WNT-ligand independent.
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| title_full_unstemmed |
Figure 4. Activation of TCF/β-catenin transcriptional activity and myogenesis by NOTCH is WNT-ligand independent.
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| title_sort |
figure 4. activation of tcf/β-catenin transcriptional activity and myogenesis by notch is wnt-ligand independent.
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| publisher |
eLife Sciences Publications, Ltd
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| publishDate |
2016
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| url |
http://dx.doi.org/10.7554/elife.14847.005
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| genre |
DML
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DML
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| op_doi |
https://doi.org/10.7554/elife.14847.005
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| _version_ |
1772181770775560192
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