Figure 6. Intracellular homo- and hetero-multimerization of mGBPs at the PVM of T. gondii.
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eLife Sciences Publications, Ltd
2016
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| Online Access: | http://dx.doi.org/10.7554/elife.11479.014 |
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crelifesciences:10.7554/elife.11479.014 |
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crelifesciences:10.7554/elife.11479.014 2023-07-23T04:22:14+02:00 Figure 6. Intracellular homo- and hetero-multimerization of mGBPs at the PVM of T. gondii. All cells were pre-treated with IFNγ for 16 hr prior investigation (a) Left panels. GFP fluorescence intensity images of living G-mGBP2 or G-mGBP2/mCh-mGBP(1,2,3,5,6) MEFs infected with T. gondii highlighted with selections of pixels within different intracellular localizations. Right panels. Two MFIS 2D-histograms of GFP <τD>f on y axes, GFP/mCherry FG/FR and photon number per pixel (N) on x axes. The pixel populations locating in cytosol (N < 1000: red island) and at the PVM (N > 1000: green island) were separated according to photon numbers. (b) Upper panel. For individual G-mGBP2, G-mGBP2/mCh-mGBP2 or G-mGBP2/mCh-mGBP6 MEFs pixel averages of rD in the cytosol and at the PVM were plotted against <τD>f or CG-mGBP2. Lower panel. Averages of <rD>loc were plotted against <τD>f and CG-mGBP2. Please refer to Figure 4c for further information on the legend and overlaid curves. (c, d) εmix(t) and ε(D,A)(t) diagrams of a representative T. gondii infected G-mGBP2/mCh-mGBP2 MEF (c) and G-mGBP2/mCh-mGBP6 MEF (d). The drop in εmix(t) curves, as marked by the arrows, represents xFRET at the PVM (blue) and in the cytosol (red). The dashed curves representing the ε(D,A)(t) diagrams of G-mGBP2/mCh-mGBP2 interactions in the cytosol (red) and VLS (green) in uninfected cells are inserted for comparison from Figure 4d. In (c), kFRET at the PVM is 0.24 ns-1. 2016-02-26T09:59:51Z http://dx.doi.org/10.7554/elife.11479.014 unknown eLife Sciences Publications, Ltd component 2016 crelifesciences https://doi.org/10.7554/elife.11479.014 2023-07-05T08:47:50Z Other/Unknown Material Green Island Red Island eLife (E-Journal - via Crossref) |
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Open Polar |
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eLife (E-Journal - via Crossref) |
| op_collection_id |
crelifesciences |
| language |
unknown |
| format |
Other/Unknown Material |
| title |
Figure 6. Intracellular homo- and hetero-multimerization of mGBPs at the PVM of T. gondii. |
| spellingShingle |
Figure 6. Intracellular homo- and hetero-multimerization of mGBPs at the PVM of T. gondii. |
| title_short |
Figure 6. Intracellular homo- and hetero-multimerization of mGBPs at the PVM of T. gondii. |
| title_full |
Figure 6. Intracellular homo- and hetero-multimerization of mGBPs at the PVM of T. gondii. |
| title_fullStr |
Figure 6. Intracellular homo- and hetero-multimerization of mGBPs at the PVM of T. gondii. |
| title_full_unstemmed |
Figure 6. Intracellular homo- and hetero-multimerization of mGBPs at the PVM of T. gondii. |
| title_sort |
figure 6. intracellular homo- and hetero-multimerization of mgbps at the pvm of t. gondii. |
| publisher |
eLife Sciences Publications, Ltd |
| publishDate |
2016 |
| url |
http://dx.doi.org/10.7554/elife.11479.014 |
| genre |
Green Island Red Island |
| genre_facet |
Green Island Red Island |
| op_doi |
https://doi.org/10.7554/elife.11479.014 |
| _version_ |
1772188970804838400 |