Novel origin of lamin-derived cytoplasmic intermediate filaments in tardigrades
Intermediate filament (IF) proteins, including nuclear lamins and cytoplasmic IF proteins, are essential cytoskeletal components of bilaterian cells. Despite their important role in protecting tissues against mechanical force, no cytoplasmic IF proteins have been convincingly identified in arthropod...
Published in: | eLife |
---|---|
Main Authors: | , , , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
eLife Sciences Publications, Ltd
2016
|
Subjects: | |
Online Access: | http://dx.doi.org/10.7554/elife.11117 https://cdn.elifesciences.org/articles/11117/elife-11117-v3.pdf https://cdn.elifesciences.org/articles/11117/elife-11117-v3.xml https://elifesciences.org/articles/11117 |
id |
crelifesciences:10.7554/elife.11117 |
---|---|
record_format |
openpolar |
spelling |
crelifesciences:10.7554/elife.11117 2024-09-15T18:41:38+00:00 Novel origin of lamin-derived cytoplasmic intermediate filaments in tardigrades Hering, Lars Bouameur, Jamal-Eddine Reichelt, Julian Magin, Thomas M Mayer, Georg Deutsche Forschungsgemeinschaft Deutsche Forschungsgemeinschaft Deutsche Forschungsgemeinschaft 2016 http://dx.doi.org/10.7554/elife.11117 https://cdn.elifesciences.org/articles/11117/elife-11117-v3.pdf https://cdn.elifesciences.org/articles/11117/elife-11117-v3.xml https://elifesciences.org/articles/11117 en eng eLife Sciences Publications, Ltd http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ eLife volume 5 ISSN 2050-084X journal-article 2016 crelifesciences https://doi.org/10.7554/elife.11117 2024-08-13T04:19:43Z Intermediate filament (IF) proteins, including nuclear lamins and cytoplasmic IF proteins, are essential cytoskeletal components of bilaterian cells. Despite their important role in protecting tissues against mechanical force, no cytoplasmic IF proteins have been convincingly identified in arthropods. Here we show that the ancestral cytoplasmic IF protein gene was lost in the entire panarthropod (onychophoran + tardigrade + arthropod) rather than arthropod lineage and that nuclear, lamin-derived proteins instead acquired new cytoplasmic roles at least three times independently in collembolans, copepods, and tardigrades. Transcriptomic and genomic data revealed three IF protein genes in the tardigrade Hypsibius dujardini, one of which (cytotardin) occurs exclusively in the cytoplasm of epidermal and foregut epithelia, where it forms belt-like filaments around each epithelial cell. These results suggest that a lamin derivative has been co-opted to enhance tissue stability in tardigrades, a function otherwise served by cytoplasmic IF proteins in all other bilaterians. Article in Journal/Newspaper Copepods Tardigrade eLife eLife 5 |
institution |
Open Polar |
collection |
eLife |
op_collection_id |
crelifesciences |
language |
English |
description |
Intermediate filament (IF) proteins, including nuclear lamins and cytoplasmic IF proteins, are essential cytoskeletal components of bilaterian cells. Despite their important role in protecting tissues against mechanical force, no cytoplasmic IF proteins have been convincingly identified in arthropods. Here we show that the ancestral cytoplasmic IF protein gene was lost in the entire panarthropod (onychophoran + tardigrade + arthropod) rather than arthropod lineage and that nuclear, lamin-derived proteins instead acquired new cytoplasmic roles at least three times independently in collembolans, copepods, and tardigrades. Transcriptomic and genomic data revealed three IF protein genes in the tardigrade Hypsibius dujardini, one of which (cytotardin) occurs exclusively in the cytoplasm of epidermal and foregut epithelia, where it forms belt-like filaments around each epithelial cell. These results suggest that a lamin derivative has been co-opted to enhance tissue stability in tardigrades, a function otherwise served by cytoplasmic IF proteins in all other bilaterians. |
author2 |
Deutsche Forschungsgemeinschaft Deutsche Forschungsgemeinschaft Deutsche Forschungsgemeinschaft |
format |
Article in Journal/Newspaper |
author |
Hering, Lars Bouameur, Jamal-Eddine Reichelt, Julian Magin, Thomas M Mayer, Georg |
spellingShingle |
Hering, Lars Bouameur, Jamal-Eddine Reichelt, Julian Magin, Thomas M Mayer, Georg Novel origin of lamin-derived cytoplasmic intermediate filaments in tardigrades |
author_facet |
Hering, Lars Bouameur, Jamal-Eddine Reichelt, Julian Magin, Thomas M Mayer, Georg |
author_sort |
Hering, Lars |
title |
Novel origin of lamin-derived cytoplasmic intermediate filaments in tardigrades |
title_short |
Novel origin of lamin-derived cytoplasmic intermediate filaments in tardigrades |
title_full |
Novel origin of lamin-derived cytoplasmic intermediate filaments in tardigrades |
title_fullStr |
Novel origin of lamin-derived cytoplasmic intermediate filaments in tardigrades |
title_full_unstemmed |
Novel origin of lamin-derived cytoplasmic intermediate filaments in tardigrades |
title_sort |
novel origin of lamin-derived cytoplasmic intermediate filaments in tardigrades |
publisher |
eLife Sciences Publications, Ltd |
publishDate |
2016 |
url |
http://dx.doi.org/10.7554/elife.11117 https://cdn.elifesciences.org/articles/11117/elife-11117-v3.pdf https://cdn.elifesciences.org/articles/11117/elife-11117-v3.xml https://elifesciences.org/articles/11117 |
genre |
Copepods Tardigrade |
genre_facet |
Copepods Tardigrade |
op_source |
eLife volume 5 ISSN 2050-084X |
op_rights |
http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.7554/elife.11117 |
container_title |
eLife |
container_volume |
5 |
_version_ |
1810486031461384192 |