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crelifesciences:10.7554/elife.06496.013
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openpolar
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crelifesciences:10.7554/elife.06496.013 2023-07-23T04:21:14+02:00 Figure 6. ORCA is a scaffold for G9a-Suv39H1 complexes. (A) a–b HA-IP in control and ORCA-depleted U2OS cells co-expressing with HA-G9a and Flag-Suv39H1. (B) a TIRF images of GFP SiMPull in control and ORCA-depleted U2OS cells co-transfected with YFP-Suv39H1 and mCherry-G9a. The same lysates incubated with biotinylated HA Ab served as the control. b Average number of YFP fluorescent molecules per imaging area (5000 μm2). c The % of mCherry-G9a pulled down by YFP Suv39H1 in control and ORCA KD. (C) a TIRF images of GFP SiMPull in U2OS cells transiently transfected with YFP-Suv39H1, mCherry-G9a, and T7-ORCA full-length or truncation mutant 1–270 or 128–647. The same lysates incubated with biotinylated HA Ab served as the control. b Average number of YFP fluorescent molecules per imaging area (5000 μm2). c The % of mCherry-G9a pulled down by YFP-Suv39H1. The % of mCherry-G9a pulled down by YFP-Suv39H1 in WT-ORCA is 25 ± 1%; 1–270 ORCA is 14 ± 3%; and 128–647 ORCA is 29 ± 6%. Scale bars, 20 μm. Error bars represent s.d., n = 3. **p < 0.01, ***p < 0.001. 2015-05-25T11:15:30Z http://dx.doi.org/10.7554/elife.06496.013 unknown eLife Sciences Publications, Ltd component 2015 crelifesciences https://doi.org/10.7554/elife.06496.013 2023-07-05T08:40:40Z Other/Unknown Material Orca eLife (E-Journal - via Crossref)
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| institution |
Open Polar
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| collection |
eLife (E-Journal - via Crossref)
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| op_collection_id |
crelifesciences
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| language |
unknown
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| format |
Other/Unknown Material
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| title |
Figure 6. ORCA is a scaffold for G9a-Suv39H1 complexes.
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| spellingShingle |
Figure 6. ORCA is a scaffold for G9a-Suv39H1 complexes.
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| title_short |
Figure 6. ORCA is a scaffold for G9a-Suv39H1 complexes.
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| title_full |
Figure 6. ORCA is a scaffold for G9a-Suv39H1 complexes.
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| title_fullStr |
Figure 6. ORCA is a scaffold for G9a-Suv39H1 complexes.
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| title_full_unstemmed |
Figure 6. ORCA is a scaffold for G9a-Suv39H1 complexes.
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| title_sort |
figure 6. orca is a scaffold for g9a-suv39h1 complexes.
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| publisher |
eLife Sciences Publications, Ltd
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| publishDate |
2015
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| url |
http://dx.doi.org/10.7554/elife.06496.013
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| genre |
Orca
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| genre_facet |
Orca
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| op_doi |
https://doi.org/10.7554/elife.06496.013
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| _version_ |
1772186555767586816
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