| id |
crelifesciences:10.7554/elife.06496.009
|
| record_format |
openpolar
|
| spelling |
crelifesciences:10.7554/elife.06496.009 2023-07-23T04:21:14+02:00 Figure 4. ORCA binds and regulates levels of H3K9 methylation. (A) a Schematic of experimental setup for peptide pulldown and analyses by SiMPull. b TIRF images of YFP-ORCA WT and 1–127 aa pulled down by H3K9 modified peptides. Note that the YFP-ORCA WT and 1–127 aa truncation mutant expressing lysates were diluted so that the concentration of the overexpressed proteins is comparable (200 and 800 times, respectively for WT and 1–127 aa). c Average number of fluorescent molecules per imaging area. Scale bars, 10 μm. (B) a Chromatin fractionation in ORCA-depleted U2OS cells followed by IB analysis of H3K9me2 and me3. b Chromatin fractionation in ORCA-depleted diploid fibroblasts, WI38 followed by IB analysis of H3K9me2 and me3. Splicing factor, SRSF1 is shown as a loading control. Error bars represent s.d., n = 3. S and S2-cytosolic; S3-nuclear soluble and MNase sensitive; P: nuclear; P3: nuclear insoluble and MNase resistant fraction. 2015-05-25T15:15:30Z http://dx.doi.org/10.7554/elife.06496.009 unknown eLife Sciences Publications, Ltd component 2015 crelifesciences https://doi.org/10.7554/elife.06496.009 2023-07-05T08:41:48Z Other/Unknown Material Orca eLife (E-Journal - via Crossref)
|
| institution |
Open Polar
|
| collection |
eLife (E-Journal - via Crossref)
|
| op_collection_id |
crelifesciences
|
| language |
unknown
|
| format |
Other/Unknown Material
|
| title |
Figure 4. ORCA binds and regulates levels of H3K9 methylation.
|
| spellingShingle |
Figure 4. ORCA binds and regulates levels of H3K9 methylation.
|
| title_short |
Figure 4. ORCA binds and regulates levels of H3K9 methylation.
|
| title_full |
Figure 4. ORCA binds and regulates levels of H3K9 methylation.
|
| title_fullStr |
Figure 4. ORCA binds and regulates levels of H3K9 methylation.
|
| title_full_unstemmed |
Figure 4. ORCA binds and regulates levels of H3K9 methylation.
|
| title_sort |
figure 4. orca binds and regulates levels of h3k9 methylation.
|
| publisher |
eLife Sciences Publications, Ltd
|
| publishDate |
2015
|
| url |
http://dx.doi.org/10.7554/elife.06496.009
|
| genre |
Orca
|
| genre_facet |
Orca
|
| op_doi |
https://doi.org/10.7554/elife.06496.009
|
| _version_ |
1772186559482691584
|