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crelifesciences:10.7554/elife.06496.006
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openpolar
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crelifesciences:10.7554/elife.06496.006 2023-07-23T04:21:14+02:00 Figure 2—figure supplement 1. WD domain of ORCA interacts with H3K9 KMTs. (A) a. IP in U2OS cells expressing various T7-ORCA mutants and FLAG-G9a using T7 Ab and analysis of G9a by IB. b. IP in U2OS cells expressing various T7-ORCA mutants and HA-Suv39H1 using T7 Ab and analysis of Suv39H1 by IB. (B) a. Cells were co-transfected CFP-LacI and YFP-G9a (negative control) or CFP-LacI-ORCA along with YFP-G9a truncation mutants. b. The % of cells with YFP-G9a truncation mutants recruited to the locus is plotted. Note the significant reduction in the recruitment of YFP-G9a (aa1-965), the mutant lacking the SET domain, to the locus. Error bars represent s.d, n=3. ****p<0.0001. span=""> <0.0001. span="">(C) Localization of H3K9me2 in CLTon cells in cells with (+) or without (-) the expression of YFP-LacI-G9a. Note the H3K9me2 accumulation in YFP-LacI-G9a expressing cells. (D) IP in U2OS cells expressing T7-ORCA and GFP-G9a-full length and ∆SET mutant using T7 Ab and analysis of GFP-G9a by IB. (E) Localization in CLTon cells expressing CFP-LacI-ORCA, of HP1α and YFP-CDK9, at heterochromatic (-Dox) as well as decondensed locus (+Dox). the expression of YFP-LacI-G9a. Note the loss of HP1α and accumulation of YFP-CDK9 upon decondensation of the locus. Scale bar, 10µm. Inset represents 150% magnification of the boxed region. (F) Tethering of YFP-LacI-Orc3 recruits Orc2 at heterochromatic (-Dox) as well as decondensed locus (+Dox). Scale bar, 10µm. Inset represents 150% magnification of the boxed region. 2015-05-25T15:15:30Z http://dx.doi.org/10.7554/elife.06496.006 unknown eLife Sciences Publications, Ltd component 2015 crelifesciences https://doi.org/10.7554/elife.06496.006 2023-07-05T08:40:28Z Other/Unknown Material Orca eLife (E-Journal - via Crossref)
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Open Polar
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| collection |
eLife (E-Journal - via Crossref)
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| op_collection_id |
crelifesciences
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| language |
unknown
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| format |
Other/Unknown Material
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| title |
Figure 2—figure supplement 1. WD domain of ORCA interacts with H3K9 KMTs.
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| spellingShingle |
Figure 2—figure supplement 1. WD domain of ORCA interacts with H3K9 KMTs.
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| title_short |
Figure 2—figure supplement 1. WD domain of ORCA interacts with H3K9 KMTs.
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| title_full |
Figure 2—figure supplement 1. WD domain of ORCA interacts with H3K9 KMTs.
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| title_fullStr |
Figure 2—figure supplement 1. WD domain of ORCA interacts with H3K9 KMTs.
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| title_full_unstemmed |
Figure 2—figure supplement 1. WD domain of ORCA interacts with H3K9 KMTs.
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| title_sort |
figure 2—figure supplement 1. wd domain of orca interacts with h3k9 kmts.
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| publisher |
eLife Sciences Publications, Ltd
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| publishDate |
2015
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| url |
http://dx.doi.org/10.7554/elife.06496.006
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| genre |
Orca
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| genre_facet |
Orca
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| op_doi |
https://doi.org/10.7554/elife.06496.006
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| _version_ |
1772186568226766848
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