| id |
crelifesciences:10.7554/elife.06496.005
|
| record_format |
openpolar
|
| spelling |
crelifesciences:10.7554/elife.06496.005 2023-07-23T04:21:14+02:00 Figure 2. ORCA associates with KMTs in a chromatin context-dependent manner. (A) a Schematic representation of various truncation mutants of ORCA containing a T7-epitope on the N-terminus. The specific domains that can associate with G9a and Suv39H1 based on–IB (Figure 2—figure supplement 1Aa,Ab) is depicted as ‘+’. b Schematic representation of various truncation mutants of G9a containing a HA-epitope on the N-terminus. The interaction domain of G9a that interacts with ORCA (Figure 2Ba) is denoted as ‘+’. c Schematic representation of various truncation mutants of Suv39H1 containing a HA-epitope on the N-terminus. The interaction domain of Suv39H1 that interacts with ORCA (Figure 2Bb) is denoted as ‘+’. (B) a IP in U2OS cells expressing various HA-G9a mutants and T7-G9a using T7 Ab and analysis by T7 and HA-IB. b IP in U2OS cells expressing various HA-Suv39H1 mutants and T7-G9a using T7 Ab and analysis by T7 and HA IB. ‘*’ denotes the cross-reacting band. (C) a Cells co-transfected with YFP-LacI (negative control) and CFP-G9a or YFP-LacI-ORCA or the truncation mutants along with CFP G9a in CLTon cells. b The % of cells with CFP-G9a recruited to the locus is plotted. (D) a Cells co-transfected with YFP-LacI (negative control) and CFP-ORCA or YFP-LacI-G9a wild type and the mutants, which are catalytically inactive along with CFP-ORCA in CLTon cells. b The % of cells with CFP-ORCA recruited to the locus. (E) a U2OS 2-6-3 CLTon cells co-transfected with YFP-LacI-ORCA and CFP-G9a in the presence and absence of doxycycline. b The % of cells with CFP-G9a recruited to the locus in both conditions. Scale bars equal 10 μm. Inset represents 150% magnification of the boxed region. Error bars represent s.d., n = 3. ****p < 0.0001. 2015-05-25T15:15:30Z http://dx.doi.org/10.7554/elife.06496.005 unknown eLife Sciences Publications, Ltd component 2015 crelifesciences https://doi.org/10.7554/elife.06496.005 2023-07-05T08:38:19Z Other/Unknown Material Orca eLife (E-Journal - via Crossref)
|
| institution |
Open Polar
|
| collection |
eLife (E-Journal - via Crossref)
|
| op_collection_id |
crelifesciences
|
| language |
unknown
|
| format |
Other/Unknown Material
|
| title |
Figure 2. ORCA associates with KMTs in a chromatin context-dependent manner.
|
| spellingShingle |
Figure 2. ORCA associates with KMTs in a chromatin context-dependent manner.
|
| title_short |
Figure 2. ORCA associates with KMTs in a chromatin context-dependent manner.
|
| title_full |
Figure 2. ORCA associates with KMTs in a chromatin context-dependent manner.
|
| title_fullStr |
Figure 2. ORCA associates with KMTs in a chromatin context-dependent manner.
|
| title_full_unstemmed |
Figure 2. ORCA associates with KMTs in a chromatin context-dependent manner.
|
| title_sort |
figure 2. orca associates with kmts in a chromatin context-dependent manner.
|
| publisher |
eLife Sciences Publications, Ltd
|
| publishDate |
2015
|
| url |
http://dx.doi.org/10.7554/elife.06496.005
|
| genre |
Orca
|
| genre_facet |
Orca
|
| op_doi |
https://doi.org/10.7554/elife.06496.005
|
| _version_ |
1772186557863690240
|