Confirming Hypoderma tarandi (Diptera: Oestridae) human ophthalmomyiasis by larval DNA barcoding
Abstract DNA barcoding is a practical tool for species identification, when morphological classification of an organism is difficult. Herein we describe the utilisation of this technique in a case of ophthalmomyiasis interna. A 12-year-old boy was infested during a summer holiday in northern Norway,...
Published in: | Acta Parasitologica |
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Walter de Gruyter GmbH
2014
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Online Access: | http://dx.doi.org/10.2478/s11686-014-0242-2 |
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crdegruytopen:10.2478/s11686-014-0242-2 2023-05-15T17:43:24+02:00 Confirming Hypoderma tarandi (Diptera: Oestridae) human ophthalmomyiasis by larval DNA barcoding Rukke, Bjørn Cholidis, Symira Johnsen, Arild Ottesen, Preben 2014 http://dx.doi.org/10.2478/s11686-014-0242-2 unknown Walter de Gruyter GmbH Acta Parasitologica volume 59, issue 2 ISSN 1896-1851 journal-article 2014 crdegruytopen https://doi.org/10.2478/s11686-014-0242-2 2017-05-04T06:26:06Z Abstract DNA barcoding is a practical tool for species identification, when morphological classification of an organism is difficult. Herein we describe the utilisation of this technique in a case of ophthalmomyiasis interna. A 12-year-old boy was infested during a summer holiday in northern Norway, while visiting an area populated with reindeer. Following medical examination, a Diptera larva was surgically removed from the boy’s eye and tentatively identified from its morphological traits as Hypoderma tarandi (L.) (Diptera: Oestridae). Ultimately, DNA barcoding confirmed this impression. The larval cytochrome c oxidase subunit 1 (COI) DNA sequence was matched with both profiles of five adult H. tarandi from the same region where the boy was infested, and other established profiles of H. tarandi in the Barcode of Life Data Systems (BOLD) identification engine. Article in Journal/Newspaper Northern Norway Sciendo (de Gruyter - via CrossRef) Norway Acta Parasitologica 59 2 |
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Sciendo (de Gruyter - via CrossRef) |
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description |
Abstract DNA barcoding is a practical tool for species identification, when morphological classification of an organism is difficult. Herein we describe the utilisation of this technique in a case of ophthalmomyiasis interna. A 12-year-old boy was infested during a summer holiday in northern Norway, while visiting an area populated with reindeer. Following medical examination, a Diptera larva was surgically removed from the boy’s eye and tentatively identified from its morphological traits as Hypoderma tarandi (L.) (Diptera: Oestridae). Ultimately, DNA barcoding confirmed this impression. The larval cytochrome c oxidase subunit 1 (COI) DNA sequence was matched with both profiles of five adult H. tarandi from the same region where the boy was infested, and other established profiles of H. tarandi in the Barcode of Life Data Systems (BOLD) identification engine. |
format |
Article in Journal/Newspaper |
author |
Rukke, Bjørn Cholidis, Symira Johnsen, Arild Ottesen, Preben |
spellingShingle |
Rukke, Bjørn Cholidis, Symira Johnsen, Arild Ottesen, Preben Confirming Hypoderma tarandi (Diptera: Oestridae) human ophthalmomyiasis by larval DNA barcoding |
author_facet |
Rukke, Bjørn Cholidis, Symira Johnsen, Arild Ottesen, Preben |
author_sort |
Rukke, Bjørn |
title |
Confirming Hypoderma tarandi (Diptera: Oestridae) human ophthalmomyiasis by larval DNA barcoding |
title_short |
Confirming Hypoderma tarandi (Diptera: Oestridae) human ophthalmomyiasis by larval DNA barcoding |
title_full |
Confirming Hypoderma tarandi (Diptera: Oestridae) human ophthalmomyiasis by larval DNA barcoding |
title_fullStr |
Confirming Hypoderma tarandi (Diptera: Oestridae) human ophthalmomyiasis by larval DNA barcoding |
title_full_unstemmed |
Confirming Hypoderma tarandi (Diptera: Oestridae) human ophthalmomyiasis by larval DNA barcoding |
title_sort |
confirming hypoderma tarandi (diptera: oestridae) human ophthalmomyiasis by larval dna barcoding |
publisher |
Walter de Gruyter GmbH |
publishDate |
2014 |
url |
http://dx.doi.org/10.2478/s11686-014-0242-2 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Northern Norway |
genre_facet |
Northern Norway |
op_source |
Acta Parasitologica volume 59, issue 2 ISSN 1896-1851 |
op_doi |
https://doi.org/10.2478/s11686-014-0242-2 |
container_title |
Acta Parasitologica |
container_volume |
59 |
container_issue |
2 |
_version_ |
1766145471557926912 |