The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation
Abstract The role of acetylcholine (ACh) as a signalling molecule in plants was investigated using a model system of Characeae cells. The effect of ACh on conductance of K+ channels in Nitella flexilis cells and on the action potential generation in Nitellopsis obtusa cells after H+-ATPase inhibitio...
Published in: | Open Life Sciences |
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Main Authors: | , , , , , |
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Walter de Gruyter GmbH
2012
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Online Access: | http://dx.doi.org/10.2478/s11535-012-0085-5 http://www.degruyter.com/view/j/biol.2012.7.issue-6/s11535-012-0085-5/s11535-012-0085-5.xml http://www.degruyter.com/view/j/biol.2012.7.issue-6/s11535-012-0085-5/s11535-012-0085-5.pdf |
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crdegruytopen:10.2478/s11535-012-0085-5 2023-05-15T17:23:49+02:00 The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation Kisnieriene, Vilma Ditchenko, Tatiana Kudryashov, Anatoly Sakalauskas, Vidmantas Yurin, Vladimir Ruksenas, Osvaldas 2012 http://dx.doi.org/10.2478/s11535-012-0085-5 http://www.degruyter.com/view/j/biol.2012.7.issue-6/s11535-012-0085-5/s11535-012-0085-5.xml http://www.degruyter.com/view/j/biol.2012.7.issue-6/s11535-012-0085-5/s11535-012-0085-5.pdf unknown Walter de Gruyter GmbH http://creativecommons.org/licenses/by-nc-nd/3.0/ CC-BY-NC-ND Open Life Sciences volume 7, issue 6 ISSN 2391-5412 journal-article 2012 crdegruytopen https://doi.org/10.2478/s11535-012-0085-5 2018-03-07T13:27:53Z Abstract The role of acetylcholine (ACh) as a signalling molecule in plants was investigated using a model system of Characeae cells. The effect of ACh on conductance of K+ channels in Nitella flexilis cells and on the action potential generation in Nitellopsis obtusa cells after H+-ATPase inhibition, where repolarization occurs after the opening of outward rectifying K+ channels, was investigated. Voltage-clamp method based on only one electrode impalement was used to evaluate the activity of separate potassium ion transport system at rest. We found that ACh at high concentrations (1 mM and 5 mM) activates K+ channels as the main membrane transport system at the resting state involved in electrogenesis of Characeaen membrane potential. We observed that ACh caused an increase in duration of AP repolarization of cells in K+ state when plasmalemma electrical characteristics are determined by large conductance K+ channels irrespective of whether AP were spontaneous or electrically evoked. These results indicate interference of ACh with electrical cellular signalling pathway in plants. Article in Journal/Newspaper Nitella flexilis Sciendo (de Gruyter - via CrossRef) Open Life Sciences 7 6 1066 1075 |
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Open Polar |
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Sciendo (de Gruyter - via CrossRef) |
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crdegruytopen |
language |
unknown |
description |
Abstract The role of acetylcholine (ACh) as a signalling molecule in plants was investigated using a model system of Characeae cells. The effect of ACh on conductance of K+ channels in Nitella flexilis cells and on the action potential generation in Nitellopsis obtusa cells after H+-ATPase inhibition, where repolarization occurs after the opening of outward rectifying K+ channels, was investigated. Voltage-clamp method based on only one electrode impalement was used to evaluate the activity of separate potassium ion transport system at rest. We found that ACh at high concentrations (1 mM and 5 mM) activates K+ channels as the main membrane transport system at the resting state involved in electrogenesis of Characeaen membrane potential. We observed that ACh caused an increase in duration of AP repolarization of cells in K+ state when plasmalemma electrical characteristics are determined by large conductance K+ channels irrespective of whether AP were spontaneous or electrically evoked. These results indicate interference of ACh with electrical cellular signalling pathway in plants. |
format |
Article in Journal/Newspaper |
author |
Kisnieriene, Vilma Ditchenko, Tatiana Kudryashov, Anatoly Sakalauskas, Vidmantas Yurin, Vladimir Ruksenas, Osvaldas |
spellingShingle |
Kisnieriene, Vilma Ditchenko, Tatiana Kudryashov, Anatoly Sakalauskas, Vidmantas Yurin, Vladimir Ruksenas, Osvaldas The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation |
author_facet |
Kisnieriene, Vilma Ditchenko, Tatiana Kudryashov, Anatoly Sakalauskas, Vidmantas Yurin, Vladimir Ruksenas, Osvaldas |
author_sort |
Kisnieriene, Vilma |
title |
The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation |
title_short |
The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation |
title_full |
The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation |
title_fullStr |
The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation |
title_full_unstemmed |
The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation |
title_sort |
effect of acetylcholine on characeae k+ channels at rest and during action potential generation |
publisher |
Walter de Gruyter GmbH |
publishDate |
2012 |
url |
http://dx.doi.org/10.2478/s11535-012-0085-5 http://www.degruyter.com/view/j/biol.2012.7.issue-6/s11535-012-0085-5/s11535-012-0085-5.xml http://www.degruyter.com/view/j/biol.2012.7.issue-6/s11535-012-0085-5/s11535-012-0085-5.pdf |
genre |
Nitella flexilis |
genre_facet |
Nitella flexilis |
op_source |
Open Life Sciences volume 7, issue 6 ISSN 2391-5412 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/3.0/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.2478/s11535-012-0085-5 |
container_title |
Open Life Sciences |
container_volume |
7 |
container_issue |
6 |
container_start_page |
1066 |
op_container_end_page |
1075 |
_version_ |
1766114280202043392 |