Detection of IncP replicon-specific regions in DNA from Antarctic microbiota
Abstract Plasmids capable of horizontal transfer contribute to the adaptability of bacteria, as they may provide genes that enable their hosts to cope with different selective pressures. Only limited information is available on plasmids from Antarctic habitats, and up until now surveys have only use...
Published in: | Open Life Sciences |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Walter de Gruyter GmbH
2007
|
Subjects: | |
Online Access: | http://dx.doi.org/10.2478/s11535-007-0025-y http://www.degruyter.com/view/j/biol.2007.2.issue-3/s11535-007-0025-y/s11535-007-0025-y.xml http://www.degruyter.com/view/j/biol.2007.2.issue-3/s11535-007-0025-y/s11535-007-0025-y.pdf |
id |
crdegruytopen:10.2478/s11535-007-0025-y |
---|---|
record_format |
openpolar |
spelling |
crdegruytopen:10.2478/s11535-007-0025-y 2023-05-15T14:11:57+02:00 Detection of IncP replicon-specific regions in DNA from Antarctic microbiota Imperio, Tatiana Bargagli, Roberto Marri, Laura 2007 http://dx.doi.org/10.2478/s11535-007-0025-y http://www.degruyter.com/view/j/biol.2007.2.issue-3/s11535-007-0025-y/s11535-007-0025-y.xml http://www.degruyter.com/view/j/biol.2007.2.issue-3/s11535-007-0025-y/s11535-007-0025-y.pdf unknown Walter de Gruyter GmbH http://creativecommons.org/licenses/by-nc-nd/3.0/ CC-BY-NC-ND Open Life Sciences volume 2, issue 3 ISSN 2391-5412 journal-article 2007 crdegruytopen https://doi.org/10.2478/s11535-007-0025-y 2018-03-07T13:26:52Z Abstract Plasmids capable of horizontal transfer contribute to the adaptability of bacteria, as they may provide genes that enable their hosts to cope with different selective pressures. Only limited information is available on plasmids from Antarctic habitats, and up until now surveys have only used traditional methods of endogenous plasmid isolation. The method based on primer systems, designed on the basis of published sequences for plasmids from different incompatibility (Inc) groups, is appropriate to detect the replicon-specific regions of corresponding plasmids in cultured bacteria, or in total community DNA, which share sufficient DNA similarity with reference plasmids at the amplified regions. In this study, we applied broad-host-range plasmid-specific primers to DNA from microbial samples collected at six different locations in Northern Victoria Land (Antarctica). DNA preparations were used as targets for PCR (polymerase chain reaction) amplification with primers for the IncP (trfA2) and IncQ (oriV ) groups. PCR products were Southern blotted and hybridized with PCR-derived probes for trfA2 and oriV regions. This approach detected the occurrence of IncP-specific sequences in eight out of fifteen DNA samples, suggesting a gene-mobilizing capacity within the original habitats. Article in Journal/Newspaper Antarc* Antarctic Antarctica Victoria Land Sciendo (de Gruyter - via CrossRef) Antarctic Victoria Land Open Life Sciences 2 3 378 384 |
institution |
Open Polar |
collection |
Sciendo (de Gruyter - via CrossRef) |
op_collection_id |
crdegruytopen |
language |
unknown |
description |
Abstract Plasmids capable of horizontal transfer contribute to the adaptability of bacteria, as they may provide genes that enable their hosts to cope with different selective pressures. Only limited information is available on plasmids from Antarctic habitats, and up until now surveys have only used traditional methods of endogenous plasmid isolation. The method based on primer systems, designed on the basis of published sequences for plasmids from different incompatibility (Inc) groups, is appropriate to detect the replicon-specific regions of corresponding plasmids in cultured bacteria, or in total community DNA, which share sufficient DNA similarity with reference plasmids at the amplified regions. In this study, we applied broad-host-range plasmid-specific primers to DNA from microbial samples collected at six different locations in Northern Victoria Land (Antarctica). DNA preparations were used as targets for PCR (polymerase chain reaction) amplification with primers for the IncP (trfA2) and IncQ (oriV ) groups. PCR products were Southern blotted and hybridized with PCR-derived probes for trfA2 and oriV regions. This approach detected the occurrence of IncP-specific sequences in eight out of fifteen DNA samples, suggesting a gene-mobilizing capacity within the original habitats. |
format |
Article in Journal/Newspaper |
author |
Imperio, Tatiana Bargagli, Roberto Marri, Laura |
spellingShingle |
Imperio, Tatiana Bargagli, Roberto Marri, Laura Detection of IncP replicon-specific regions in DNA from Antarctic microbiota |
author_facet |
Imperio, Tatiana Bargagli, Roberto Marri, Laura |
author_sort |
Imperio, Tatiana |
title |
Detection of IncP replicon-specific regions in DNA from Antarctic microbiota |
title_short |
Detection of IncP replicon-specific regions in DNA from Antarctic microbiota |
title_full |
Detection of IncP replicon-specific regions in DNA from Antarctic microbiota |
title_fullStr |
Detection of IncP replicon-specific regions in DNA from Antarctic microbiota |
title_full_unstemmed |
Detection of IncP replicon-specific regions in DNA from Antarctic microbiota |
title_sort |
detection of incp replicon-specific regions in dna from antarctic microbiota |
publisher |
Walter de Gruyter GmbH |
publishDate |
2007 |
url |
http://dx.doi.org/10.2478/s11535-007-0025-y http://www.degruyter.com/view/j/biol.2007.2.issue-3/s11535-007-0025-y/s11535-007-0025-y.xml http://www.degruyter.com/view/j/biol.2007.2.issue-3/s11535-007-0025-y/s11535-007-0025-y.pdf |
geographic |
Antarctic Victoria Land |
geographic_facet |
Antarctic Victoria Land |
genre |
Antarc* Antarctic Antarctica Victoria Land |
genre_facet |
Antarc* Antarctic Antarctica Victoria Land |
op_source |
Open Life Sciences volume 2, issue 3 ISSN 2391-5412 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/3.0/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.2478/s11535-007-0025-y |
container_title |
Open Life Sciences |
container_volume |
2 |
container_issue |
3 |
container_start_page |
378 |
op_container_end_page |
384 |
_version_ |
1766284185920602112 |