Retinol, cholecalciferol and alpha-tocopherol contents of Bulgarian Black Sea fish species

• Published in: Analele Universitatii "Ovidius" Constanta - Seria Chimie
• Main Authors: Stancheva, Mona, Dobreva, Diana A., Galunska, Bistra
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Walter de Gruyter GmbH 2012
• Subjects: Turbot
• Online Access: http://dx.doi.org/10.2478/v10310-012-0004-7; https://content.sciendo.com/view/journals/auoc/23/1/article-p31.xml; https://content.sciendo.com/downloadpdf/journals/auoc/23/1/article-p31.xml

Abstract The aim of the present study is to determine and to compare the content of retinol, cholecalciferol and alpha-tocopherol in edible tissue of two Black sea fishes - Garfish ( Belone belone ) and Turbot ( Psetta maxima ). All-trans-retinol (vitamin A), cholecalciferol (vitamin D 3 ) and alpha-tocopherol (vitamin E) were analyzed simultaneously using HPLC/UV/FL system (Thermo Scientific Spectra SYSTEM) equipped with RP analytical column. The mobile phase was composed of 97:3 = MeOH:H2O. Retinol and cholecalciferol were monitored by UV detection at lmax = 325 nm and lmax = 265 nm, respectively. Alpha-tocopherol was detected by fluorescence at lex=288 nm and lem=332 nm. The sample preparation procedure includes alkaline saponification, followed by liquid-liquid extraction. Quantities of all-trans-retinol and cholecalciferol were higher in garfish tissues while alpha-tocopherol content in turbot showed seven times higher values.