Retinol, cholecalciferol and alpha-tocopherol contents of Bulgarian Black Sea fish species
Abstract The aim of the present study is to determine and to compare the content of retinol, cholecalciferol and alpha-tocopherol in edible tissue of two Black sea fishes - Garfish ( Belone belone ) and Turbot ( Psetta maxima ). All-trans-retinol (vitamin A), cholecalciferol (vitamin D 3 ) and alpha...
| Published in: | Analele Universitatii "Ovidius" Constanta - Seria Chimie |
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| Main Authors: | , , |
| Format: | Article in Journal/Newspaper |
| Language: | unknown |
| Published: |
Walter de Gruyter GmbH
2012
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.2478/v10310-012-0004-7 https://content.sciendo.com/view/journals/auoc/23/1/article-p31.xml https://content.sciendo.com/downloadpdf/journals/auoc/23/1/article-p31.xml |
| Summary: | Abstract The aim of the present study is to determine and to compare the content of retinol, cholecalciferol and alpha-tocopherol in edible tissue of two Black sea fishes - Garfish ( Belone belone ) and Turbot ( Psetta maxima ). All-trans-retinol (vitamin A), cholecalciferol (vitamin D 3 ) and alpha-tocopherol (vitamin E) were analyzed simultaneously using HPLC/UV/FL system (Thermo Scientific Spectra SYSTEM) equipped with RP analytical column. The mobile phase was composed of 97:3 = MeOH:H2O. Retinol and cholecalciferol were monitored by UV detection at lmax = 325 nm and lmax = 265 nm, respectively. Alpha-tocopherol was detected by fluorescence at lex=288 nm and lem=332 nm. The sample preparation procedure includes alkaline saponification, followed by liquid-liquid extraction. Quantities of all-trans-retinol and cholecalciferol were higher in garfish tissues while alpha-tocopherol content in turbot showed seven times higher values. |
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