Analysis of cultivable aerobic bacteria isolated from bottom sediments in the Wijdefjorden region, Spitsbergen
Analysis of cultivable aerobic bacteria isolated from bottom sediments in the Wijdefjorden region, Spitsbergen The paper presents the first physicochemical and microbiological studies conducted in the northern area of Svalbard (Spitsbergen). Ten sediment samples were collected from the bottom of the...
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Walter de Gruyter GmbH
2011
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crdegruyter:10.2478/v10183-011-0012-x 2023-05-15T18:02:38+02:00 Analysis of cultivable aerobic bacteria isolated from bottom sediments in the Wijdefjorden region, Spitsbergen Konieczna, Iwona Wojtasik, Barbara Kwinkowski, Marek Burska, Dorota Nowiński, Kamil Żarnowiec, Paulina Kaca, Wiesław 2011 http://dx.doi.org/10.2478/v10183-011-0012-x https://content.sciendo.com/view/journals/popore/32/2/article-p181.xml https://www.degruyter.com/view/j/popore.2011.32.issue-2/v10183-011-0012-x/v10183-011-0012-x.pdf unknown Walter de Gruyter GmbH Polish Polar Research volume 32, issue 2, page 181-195 ISSN 2081-8262 0138-0338 Ecology Ecology, Evolution, Behavior and Systematics journal-article 2011 crdegruyter https://doi.org/10.2478/v10183-011-0012-x 2022-04-14T05:13:15Z Analysis of cultivable aerobic bacteria isolated from bottom sediments in the Wijdefjorden region, Spitsbergen The paper presents the first physicochemical and microbiological studies conducted in the northern area of Svalbard (Spitsbergen). Ten sediment samples were collected from the bottom of the longest fjord in the region, Wijdefjorden. Bottom sediments from ten lakes lo- cated along the shores of Wijdefjorden and Woodfjorden were also sampled. Organic matter content (LOI), water content, temperature, pH, and salinity of the sediments were determined. The quantity of aerobic bacteria cultured on various growth media at 4°C, 14°C, and 37°C ranged from 10 2 to 10 6 cfu/g of wet sediment mass, depending on the type of sampling station (fjord or lake). The number of bacteria did not correlate with organic matter content. Out of the 37 bacterial strains isolated from Wijdefjorden, 48% and 70% revealed ureolytic and proteolytic activity, respectively. The proportion of freshwater strains with ureolytic and proteolytic activitywas 32% and 55%, respectively. Antibiotic resistance testing indicated that bacterial strains from the bottom sediments of the lakes were resistant to 8 antibiotics (out of the 18 investigated). Possible sources of this resistance are discussed. Using 16S DNA analysis, bacterial isolates from the lakes were identified as Pseudomonas sp., whereas frequently occurring strains in bottom sediment of the fjord were Pseudoalteromonas sp. Article in Journal/Newspaper Polar Research Svalbard Wijdefjord* Woodfjord* Woodfjorden Spitsbergen De Gruyter (via Crossref) Svalbard Woodfjorden ENVELOPE(14.000,14.000,79.833,79.833) Wijdefjorden ENVELOPE(15.569,15.569,79.543,79.543) Polish Polar Research 32 2 181 195 |
institution |
Open Polar |
collection |
De Gruyter (via Crossref) |
op_collection_id |
crdegruyter |
language |
unknown |
topic |
Ecology Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Ecology Ecology, Evolution, Behavior and Systematics Konieczna, Iwona Wojtasik, Barbara Kwinkowski, Marek Burska, Dorota Nowiński, Kamil Żarnowiec, Paulina Kaca, Wiesław Analysis of cultivable aerobic bacteria isolated from bottom sediments in the Wijdefjorden region, Spitsbergen |
topic_facet |
Ecology Ecology, Evolution, Behavior and Systematics |
description |
Analysis of cultivable aerobic bacteria isolated from bottom sediments in the Wijdefjorden region, Spitsbergen The paper presents the first physicochemical and microbiological studies conducted in the northern area of Svalbard (Spitsbergen). Ten sediment samples were collected from the bottom of the longest fjord in the region, Wijdefjorden. Bottom sediments from ten lakes lo- cated along the shores of Wijdefjorden and Woodfjorden were also sampled. Organic matter content (LOI), water content, temperature, pH, and salinity of the sediments were determined. The quantity of aerobic bacteria cultured on various growth media at 4°C, 14°C, and 37°C ranged from 10 2 to 10 6 cfu/g of wet sediment mass, depending on the type of sampling station (fjord or lake). The number of bacteria did not correlate with organic matter content. Out of the 37 bacterial strains isolated from Wijdefjorden, 48% and 70% revealed ureolytic and proteolytic activity, respectively. The proportion of freshwater strains with ureolytic and proteolytic activitywas 32% and 55%, respectively. Antibiotic resistance testing indicated that bacterial strains from the bottom sediments of the lakes were resistant to 8 antibiotics (out of the 18 investigated). Possible sources of this resistance are discussed. Using 16S DNA analysis, bacterial isolates from the lakes were identified as Pseudomonas sp., whereas frequently occurring strains in bottom sediment of the fjord were Pseudoalteromonas sp. |
format |
Article in Journal/Newspaper |
author |
Konieczna, Iwona Wojtasik, Barbara Kwinkowski, Marek Burska, Dorota Nowiński, Kamil Żarnowiec, Paulina Kaca, Wiesław |
author_facet |
Konieczna, Iwona Wojtasik, Barbara Kwinkowski, Marek Burska, Dorota Nowiński, Kamil Żarnowiec, Paulina Kaca, Wiesław |
author_sort |
Konieczna, Iwona |
title |
Analysis of cultivable aerobic bacteria isolated from bottom sediments in the Wijdefjorden region, Spitsbergen |
title_short |
Analysis of cultivable aerobic bacteria isolated from bottom sediments in the Wijdefjorden region, Spitsbergen |
title_full |
Analysis of cultivable aerobic bacteria isolated from bottom sediments in the Wijdefjorden region, Spitsbergen |
title_fullStr |
Analysis of cultivable aerobic bacteria isolated from bottom sediments in the Wijdefjorden region, Spitsbergen |
title_full_unstemmed |
Analysis of cultivable aerobic bacteria isolated from bottom sediments in the Wijdefjorden region, Spitsbergen |
title_sort |
analysis of cultivable aerobic bacteria isolated from bottom sediments in the wijdefjorden region, spitsbergen |
publisher |
Walter de Gruyter GmbH |
publishDate |
2011 |
url |
http://dx.doi.org/10.2478/v10183-011-0012-x https://content.sciendo.com/view/journals/popore/32/2/article-p181.xml https://www.degruyter.com/view/j/popore.2011.32.issue-2/v10183-011-0012-x/v10183-011-0012-x.pdf |
long_lat |
ENVELOPE(14.000,14.000,79.833,79.833) ENVELOPE(15.569,15.569,79.543,79.543) |
geographic |
Svalbard Woodfjorden Wijdefjorden |
geographic_facet |
Svalbard Woodfjorden Wijdefjorden |
genre |
Polar Research Svalbard Wijdefjord* Woodfjord* Woodfjorden Spitsbergen |
genre_facet |
Polar Research Svalbard Wijdefjord* Woodfjord* Woodfjorden Spitsbergen |
op_source |
Polish Polar Research volume 32, issue 2, page 181-195 ISSN 2081-8262 0138-0338 |
op_doi |
https://doi.org/10.2478/v10183-011-0012-x |
container_title |
Polish Polar Research |
container_volume |
32 |
container_issue |
2 |
container_start_page |
181 |
op_container_end_page |
195 |
_version_ |
1766173046197977088 |