Cross-Species Applicability of Microsatellite Markers for Investigation of Sea Ducks (Mergini) Genetic Differentiation
Cross-Species Applicability of Microsatellite Markers for Investigation of Sea Ducks (Mergini) Genetic Differentiation Genetic studies of waterfowl have provided insufficient information on the evolutionary history of the sea duck tribe (Mergini, Anseriformes), as highly variable molecular markers h...
Published in: | Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. |
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Walter de Gruyter GmbH
2008
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crdegruyter:10.2478/v10046-009-0002-0 2023-05-15T17:10:55+02:00 Cross-Species Applicability of Microsatellite Markers for Investigation of Sea Ducks (Mergini) Genetic Differentiation Sruoga, Aniolas Slavėnaitė, Sigita Butkauskas, Dalius Gražulevičius, Gediminas 2008 http://dx.doi.org/10.2478/v10046-009-0002-0 https://content.sciendo.com/view/journals/prolas/62/6/article-p215.xml https://www.degruyter.com/view/j/prolas.2008.62.issue-6/v10046-009-0002-0/v10046-009-0002-0.pdf unknown Walter de Gruyter GmbH Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. volume 62, issue 6, page 215-218 ISSN 1407-009X Multidisciplinary journal-article 2008 crdegruyter https://doi.org/10.2478/v10046-009-0002-0 2022-04-14T05:07:40Z Cross-Species Applicability of Microsatellite Markers for Investigation of Sea Ducks (Mergini) Genetic Differentiation Genetic studies of waterfowl have provided insufficient information on the evolutionary history of the sea duck tribe (Mergini, Anseriformes), as highly variable molecular markers have not been identified. Cross-species applicability of microsatellites has been shown for several bird families. Therefore, the objective of our work was to examine whether specific primers used previously for Anatidae, Phasianidae and Laridae taxons could amplify microsatellite loci of sea duck species: Long-tailed duck (Clangula hyemalis), Goosander (Mergus merganser) and Velvet Scoter (Melanitta fusca). Tissue samples were collected and DNA was extracted by rapid salt extraction method. Amplification of DNA fragments was carried out using specific microsatellite primers of APH21, Aalmu1, Sfimu4, Sfimu5 (Anatidae), ADL209, ADL115 (Phasianidae) and K71, RGB28 (Laridae). Four primer pairs (APH21, Aalmu1, K71, and nSfimu4) were suitable for investigation of interspecies genetic variability among Long-tailed duck and Velvet Scoter. Intraspecies specificity has been detected for primer pair ADL 209 in all three duck species. The primer pair APH21 was selected as most promising for investigation of intraspecies variability of Long-tailed duck and Velvet Scoter . Article in Journal/Newspaper Melanitta fusca velvet scoter De Gruyter (via Crossref) Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. 62 6 215 218 |
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topic |
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Multidisciplinary Sruoga, Aniolas Slavėnaitė, Sigita Butkauskas, Dalius Gražulevičius, Gediminas Cross-Species Applicability of Microsatellite Markers for Investigation of Sea Ducks (Mergini) Genetic Differentiation |
topic_facet |
Multidisciplinary |
description |
Cross-Species Applicability of Microsatellite Markers for Investigation of Sea Ducks (Mergini) Genetic Differentiation Genetic studies of waterfowl have provided insufficient information on the evolutionary history of the sea duck tribe (Mergini, Anseriformes), as highly variable molecular markers have not been identified. Cross-species applicability of microsatellites has been shown for several bird families. Therefore, the objective of our work was to examine whether specific primers used previously for Anatidae, Phasianidae and Laridae taxons could amplify microsatellite loci of sea duck species: Long-tailed duck (Clangula hyemalis), Goosander (Mergus merganser) and Velvet Scoter (Melanitta fusca). Tissue samples were collected and DNA was extracted by rapid salt extraction method. Amplification of DNA fragments was carried out using specific microsatellite primers of APH21, Aalmu1, Sfimu4, Sfimu5 (Anatidae), ADL209, ADL115 (Phasianidae) and K71, RGB28 (Laridae). Four primer pairs (APH21, Aalmu1, K71, and nSfimu4) were suitable for investigation of interspecies genetic variability among Long-tailed duck and Velvet Scoter. Intraspecies specificity has been detected for primer pair ADL 209 in all three duck species. The primer pair APH21 was selected as most promising for investigation of intraspecies variability of Long-tailed duck and Velvet Scoter . |
format |
Article in Journal/Newspaper |
author |
Sruoga, Aniolas Slavėnaitė, Sigita Butkauskas, Dalius Gražulevičius, Gediminas |
author_facet |
Sruoga, Aniolas Slavėnaitė, Sigita Butkauskas, Dalius Gražulevičius, Gediminas |
author_sort |
Sruoga, Aniolas |
title |
Cross-Species Applicability of Microsatellite Markers for Investigation of Sea Ducks (Mergini) Genetic Differentiation |
title_short |
Cross-Species Applicability of Microsatellite Markers for Investigation of Sea Ducks (Mergini) Genetic Differentiation |
title_full |
Cross-Species Applicability of Microsatellite Markers for Investigation of Sea Ducks (Mergini) Genetic Differentiation |
title_fullStr |
Cross-Species Applicability of Microsatellite Markers for Investigation of Sea Ducks (Mergini) Genetic Differentiation |
title_full_unstemmed |
Cross-Species Applicability of Microsatellite Markers for Investigation of Sea Ducks (Mergini) Genetic Differentiation |
title_sort |
cross-species applicability of microsatellite markers for investigation of sea ducks (mergini) genetic differentiation |
publisher |
Walter de Gruyter GmbH |
publishDate |
2008 |
url |
http://dx.doi.org/10.2478/v10046-009-0002-0 https://content.sciendo.com/view/journals/prolas/62/6/article-p215.xml https://www.degruyter.com/view/j/prolas.2008.62.issue-6/v10046-009-0002-0/v10046-009-0002-0.pdf |
genre |
Melanitta fusca velvet scoter |
genre_facet |
Melanitta fusca velvet scoter |
op_source |
Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. volume 62, issue 6, page 215-218 ISSN 1407-009X |
op_doi |
https://doi.org/10.2478/v10046-009-0002-0 |
container_title |
Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. |
container_volume |
62 |
container_issue |
6 |
container_start_page |
215 |
op_container_end_page |
218 |
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1766067583088328704 |