Comparative analyses of different genetic markers for the detection of Acanthamoeba spp. isolates
Abstract Acanthamoeba are widespread free-living amoebae which may cause granulomatous amoebic encephalitis (GAE), keratitis, skin ulcerations and disseminated tissue infection. An important diagnostic and prognostic factor for the treatment of infection is a quick and correct diagnosis of amoebae s...
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Walter de Gruyter GmbH
2014
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Online Access: | http://dx.doi.org/10.2478/s11686-014-0267-6 http://link.springer.com/content/pdf/10.2478/s11686-014-0267-6.pdf http://link.springer.com/article/10.2478/s11686-014-0267-6/fulltext.html http://link.springer.com/content/pdf/10.2478/s11686-014-0267-6 |
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crdegruyter:10.2478/s11686-014-0267-6 2023-05-15T16:50:32+02:00 Comparative analyses of different genetic markers for the detection of Acanthamoeba spp. isolates Derda, Monika Wojtkowiak-Giera, Agnieszka Hadaś, Edward 2014 http://dx.doi.org/10.2478/s11686-014-0267-6 http://link.springer.com/content/pdf/10.2478/s11686-014-0267-6.pdf http://link.springer.com/article/10.2478/s11686-014-0267-6/fulltext.html http://link.springer.com/content/pdf/10.2478/s11686-014-0267-6 unknown Walter de Gruyter GmbH http://www.springer.com/tdm Acta Parasitologica volume 59, issue 3 ISSN 1896-1851 Parasitology journal-article 2014 crdegruyter https://doi.org/10.2478/s11686-014-0267-6 2022-04-14T05:13:38Z Abstract Acanthamoeba are widespread free-living amoebae which may cause granulomatous amoebic encephalitis (GAE), keratitis, skin ulcerations and disseminated tissue infection. An important diagnostic and prognostic factor for the treatment of infection is a quick and correct diagnosis of amoebae strains. The aim of our study was to develop a rapid method for detection and identification of pathogenic Acanthamoeba spp. strains from diagnostic material collected from water. In this study we analysed five amplification-based genetic markers (Aca 16S, Ac6/210, GP, JDP, Nelson) used for identification of pathogenic Acanthamoeba spp. strains isolated in water sources in Poland, Iceland and Sweden. Our results demonstrated the presence of pathogenic Acanthamoeba strains in tap water. PCR assay appeared to be a more rapid and sensitive method to detect the presence of amoebae than the limited conventional techniques. Based on our observations, we can confirm that the use of four out of five genetic markers (Aca 16S, Ac 6/210, JDP, GP, Nelson) may be helpful in identification of Acanthamoeba spp. strains, but only one Aca 16S primer pair is a highly specific marker that distinguishes between pathogenic strains of Acanthamoeba and other free-living amoeba families. Article in Journal/Newspaper Iceland De Gruyter (via Crossref) Acta Parasitologica 59 3 |
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topic |
Parasitology |
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Parasitology Derda, Monika Wojtkowiak-Giera, Agnieszka Hadaś, Edward Comparative analyses of different genetic markers for the detection of Acanthamoeba spp. isolates |
topic_facet |
Parasitology |
description |
Abstract Acanthamoeba are widespread free-living amoebae which may cause granulomatous amoebic encephalitis (GAE), keratitis, skin ulcerations and disseminated tissue infection. An important diagnostic and prognostic factor for the treatment of infection is a quick and correct diagnosis of amoebae strains. The aim of our study was to develop a rapid method for detection and identification of pathogenic Acanthamoeba spp. strains from diagnostic material collected from water. In this study we analysed five amplification-based genetic markers (Aca 16S, Ac6/210, GP, JDP, Nelson) used for identification of pathogenic Acanthamoeba spp. strains isolated in water sources in Poland, Iceland and Sweden. Our results demonstrated the presence of pathogenic Acanthamoeba strains in tap water. PCR assay appeared to be a more rapid and sensitive method to detect the presence of amoebae than the limited conventional techniques. Based on our observations, we can confirm that the use of four out of five genetic markers (Aca 16S, Ac 6/210, JDP, GP, Nelson) may be helpful in identification of Acanthamoeba spp. strains, but only one Aca 16S primer pair is a highly specific marker that distinguishes between pathogenic strains of Acanthamoeba and other free-living amoeba families. |
format |
Article in Journal/Newspaper |
author |
Derda, Monika Wojtkowiak-Giera, Agnieszka Hadaś, Edward |
author_facet |
Derda, Monika Wojtkowiak-Giera, Agnieszka Hadaś, Edward |
author_sort |
Derda, Monika |
title |
Comparative analyses of different genetic markers for the detection of Acanthamoeba spp. isolates |
title_short |
Comparative analyses of different genetic markers for the detection of Acanthamoeba spp. isolates |
title_full |
Comparative analyses of different genetic markers for the detection of Acanthamoeba spp. isolates |
title_fullStr |
Comparative analyses of different genetic markers for the detection of Acanthamoeba spp. isolates |
title_full_unstemmed |
Comparative analyses of different genetic markers for the detection of Acanthamoeba spp. isolates |
title_sort |
comparative analyses of different genetic markers for the detection of acanthamoeba spp. isolates |
publisher |
Walter de Gruyter GmbH |
publishDate |
2014 |
url |
http://dx.doi.org/10.2478/s11686-014-0267-6 http://link.springer.com/content/pdf/10.2478/s11686-014-0267-6.pdf http://link.springer.com/article/10.2478/s11686-014-0267-6/fulltext.html http://link.springer.com/content/pdf/10.2478/s11686-014-0267-6 |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Acta Parasitologica volume 59, issue 3 ISSN 1896-1851 |
op_rights |
http://www.springer.com/tdm |
op_doi |
https://doi.org/10.2478/s11686-014-0267-6 |
container_title |
Acta Parasitologica |
container_volume |
59 |
container_issue |
3 |
_version_ |
1766040668596076544 |