Assessment of iPBS Primers Potential to Be Used in Genetic Diversity Studies of Wild Cloudberry ( Rubus Chamaemorus L.) Populations
Abstract Inter-primer binding site (iPBS) molecular retrotransposon-based markers are a universal and simple tool to estimate genetic diversity. The goal of this study was to select highly informative iPBS primers to be used in studies of intraspecific genetic diversity of cloudberry (Rubus chamaemo...
Published in: | Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. |
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Walter de Gruyter GmbH
2022
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Online Access: | http://dx.doi.org/10.2478/prolas-2022-0045 https://www.sciendo.com/pdf/10.2478/prolas-2022-0045 |
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crdegruyter:10.2478/prolas-2022-0045 2024-05-19T07:47:51+00:00 Assessment of iPBS Primers Potential to Be Used in Genetic Diversity Studies of Wild Cloudberry ( Rubus Chamaemorus L.) Populations Krasņevska, Nikole Miķelsone, Andra Kruchonok, Alesia Rashal, Isaak Butkauskas, Dalius Grauda, Dace 2022 http://dx.doi.org/10.2478/prolas-2022-0045 https://www.sciendo.com/pdf/10.2478/prolas-2022-0045 en eng Walter de Gruyter GmbH http://creativecommons.org/licenses/by-nc-nd/3.0 Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. volume 76, issue 2, page 314-316 ISSN 2255-890X journal-article 2022 crdegruyter https://doi.org/10.2478/prolas-2022-0045 2024-05-02T06:52:08Z Abstract Inter-primer binding site (iPBS) molecular retrotransposon-based markers are a universal and simple tool to estimate genetic diversity. The goal of this study was to select highly informative iPBS primers to be used in studies of intraspecific genetic diversity of cloudberry (Rubus chamaemorus L.), a common plant species of the boreal zone, associated with raised bog habitats and transitional bogs. Initially, 75 iPBS primers were screened and three of the most informative primers (indicated by numbers 2298, 2277, and 2229) were selected based on the highest number of polymorphic bands and PIC (polymorphic information content) values. Electrophoretic analysis revealed 34 bands amplified with primer 2229, 25 bands with primer 2298, and 22 bands with primer 2277. The percentage of polymorphic loci ranged from 90.9 to 97.1% and PIC values ranged from 0.4885 to 0.4965, indicating potential of the selected primers to be applied in population genetic studies of R. chamaemorus. Article in Journal/Newspaper Rubus chamaemorus De Gruyter Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. 76 2 314 316 |
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English |
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Abstract Inter-primer binding site (iPBS) molecular retrotransposon-based markers are a universal and simple tool to estimate genetic diversity. The goal of this study was to select highly informative iPBS primers to be used in studies of intraspecific genetic diversity of cloudberry (Rubus chamaemorus L.), a common plant species of the boreal zone, associated with raised bog habitats and transitional bogs. Initially, 75 iPBS primers were screened and three of the most informative primers (indicated by numbers 2298, 2277, and 2229) were selected based on the highest number of polymorphic bands and PIC (polymorphic information content) values. Electrophoretic analysis revealed 34 bands amplified with primer 2229, 25 bands with primer 2298, and 22 bands with primer 2277. The percentage of polymorphic loci ranged from 90.9 to 97.1% and PIC values ranged from 0.4885 to 0.4965, indicating potential of the selected primers to be applied in population genetic studies of R. chamaemorus. |
format |
Article in Journal/Newspaper |
author |
Krasņevska, Nikole Miķelsone, Andra Kruchonok, Alesia Rashal, Isaak Butkauskas, Dalius Grauda, Dace |
spellingShingle |
Krasņevska, Nikole Miķelsone, Andra Kruchonok, Alesia Rashal, Isaak Butkauskas, Dalius Grauda, Dace Assessment of iPBS Primers Potential to Be Used in Genetic Diversity Studies of Wild Cloudberry ( Rubus Chamaemorus L.) Populations |
author_facet |
Krasņevska, Nikole Miķelsone, Andra Kruchonok, Alesia Rashal, Isaak Butkauskas, Dalius Grauda, Dace |
author_sort |
Krasņevska, Nikole |
title |
Assessment of iPBS Primers Potential to Be Used in Genetic Diversity Studies of Wild Cloudberry ( Rubus Chamaemorus L.) Populations |
title_short |
Assessment of iPBS Primers Potential to Be Used in Genetic Diversity Studies of Wild Cloudberry ( Rubus Chamaemorus L.) Populations |
title_full |
Assessment of iPBS Primers Potential to Be Used in Genetic Diversity Studies of Wild Cloudberry ( Rubus Chamaemorus L.) Populations |
title_fullStr |
Assessment of iPBS Primers Potential to Be Used in Genetic Diversity Studies of Wild Cloudberry ( Rubus Chamaemorus L.) Populations |
title_full_unstemmed |
Assessment of iPBS Primers Potential to Be Used in Genetic Diversity Studies of Wild Cloudberry ( Rubus Chamaemorus L.) Populations |
title_sort |
assessment of ipbs primers potential to be used in genetic diversity studies of wild cloudberry ( rubus chamaemorus l.) populations |
publisher |
Walter de Gruyter GmbH |
publishDate |
2022 |
url |
http://dx.doi.org/10.2478/prolas-2022-0045 https://www.sciendo.com/pdf/10.2478/prolas-2022-0045 |
genre |
Rubus chamaemorus |
genre_facet |
Rubus chamaemorus |
op_source |
Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. volume 76, issue 2, page 314-316 ISSN 2255-890X |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/3.0 |
op_doi |
https://doi.org/10.2478/prolas-2022-0045 |
container_title |
Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. |
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76 |
container_issue |
2 |
container_start_page |
314 |
op_container_end_page |
316 |
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1799488337671618560 |