Characterization of heavy metals resistant heterotrophic bacteria from soils in the Windmill Islands region, Wilkes Land, East Antarctica
Abstract In this study, selected heavy metals resistant heterotrophic bacteria isolated from soil samples at the Windmill Islands region, Wilkes Land (East Antarctica), were characterized. Phylogenetic analysis revealed affiliation of isolates to genera Bacillus, Lysinibacillus, Micrococcus and Sten...
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Walter de Gruyter GmbH
2014
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crdegruyter:10.2478/popore-2014-0028 2023-05-15T14:12:18+02:00 Characterization of heavy metals resistant heterotrophic bacteria from soils in the Windmill Islands region, Wilkes Land, East Antarctica Tomova, Iva Stoilova−Disheva, Margarita Vasileva−Tonkova, Evgenia 2014 http://dx.doi.org/10.2478/popore-2014-0028 http://content.sciendo.com/view/journals/popore/35/4/article-p593.xml https://www.degruyter.com/view/j/popore.2014.35.issue-4/popore-2014-0028/popore-2014-0028.pdf unknown Walter de Gruyter GmbH http://creativecommons.org/licenses/by-nc-nd/3.0/ CC-BY-NC-ND Polish Polar Research volume 35, issue 4, page 593-607 ISSN 2081-8262 Ecology Ecology, Evolution, Behavior and Systematics journal-article 2014 crdegruyter https://doi.org/10.2478/popore-2014-0028 2022-07-08T14:12:07Z Abstract In this study, selected heavy metals resistant heterotrophic bacteria isolated from soil samples at the Windmill Islands region, Wilkes Land (East Antarctica), were characterized. Phylogenetic analysis revealed affiliation of isolates to genera Bacillus, Lysinibacillus, Micrococcus and Stenotrophomonas. The strains were found to be psychrotolerant and halotolerant, able to tolerate up to 10% NaCl in the growth medium. The Minimum Inhibitory Concentration of the seven heavy metals Cr, Cu, Ni, Co, Cd, Zn, and Pb was determined in solid media for each bacterial strain. Gram−positive Vi−2 strain and Gram−negative Vi−4 strain showed highest multiply heavy metals resistance, and Vi−3 and Vi−4 strains showed multi−antibiotic resistance to more than a half of the 13 used antibiotics. Plasmids were detected only in Gram−negative Vi−4 strain. The bacteria were able to produce different hydrolytic enzymes including industrially important proteases, xylanases, cellulases, and β−glucosidases. High heavy metals resistance of the Antarctic bacteria suggests their potential application for wastewater treatment in cold and temperate climates. Highly sensitive to Cd and Co ions Vi−1, Vi−5 and Vi−7 strains would be promising for developing biosensors to detect these most toxic heavy metals in environmental samples. Article in Journal/Newspaper Antarc* Antarctic Antarctica East Antarctica Polar Research Wilkes Land Windmill Islands De Gruyter (via Crossref) Antarctic The Antarctic East Antarctica Wilkes Land ENVELOPE(120.000,120.000,-69.000,-69.000) Windmill Islands ENVELOPE(110.417,110.417,-66.350,-66.350) Polish Polar Research 35 4 593 607 |
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Open Polar |
collection |
De Gruyter (via Crossref) |
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unknown |
topic |
Ecology Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Ecology Ecology, Evolution, Behavior and Systematics Tomova, Iva Stoilova−Disheva, Margarita Vasileva−Tonkova, Evgenia Characterization of heavy metals resistant heterotrophic bacteria from soils in the Windmill Islands region, Wilkes Land, East Antarctica |
topic_facet |
Ecology Ecology, Evolution, Behavior and Systematics |
description |
Abstract In this study, selected heavy metals resistant heterotrophic bacteria isolated from soil samples at the Windmill Islands region, Wilkes Land (East Antarctica), were characterized. Phylogenetic analysis revealed affiliation of isolates to genera Bacillus, Lysinibacillus, Micrococcus and Stenotrophomonas. The strains were found to be psychrotolerant and halotolerant, able to tolerate up to 10% NaCl in the growth medium. The Minimum Inhibitory Concentration of the seven heavy metals Cr, Cu, Ni, Co, Cd, Zn, and Pb was determined in solid media for each bacterial strain. Gram−positive Vi−2 strain and Gram−negative Vi−4 strain showed highest multiply heavy metals resistance, and Vi−3 and Vi−4 strains showed multi−antibiotic resistance to more than a half of the 13 used antibiotics. Plasmids were detected only in Gram−negative Vi−4 strain. The bacteria were able to produce different hydrolytic enzymes including industrially important proteases, xylanases, cellulases, and β−glucosidases. High heavy metals resistance of the Antarctic bacteria suggests their potential application for wastewater treatment in cold and temperate climates. Highly sensitive to Cd and Co ions Vi−1, Vi−5 and Vi−7 strains would be promising for developing biosensors to detect these most toxic heavy metals in environmental samples. |
format |
Article in Journal/Newspaper |
author |
Tomova, Iva Stoilova−Disheva, Margarita Vasileva−Tonkova, Evgenia |
author_facet |
Tomova, Iva Stoilova−Disheva, Margarita Vasileva−Tonkova, Evgenia |
author_sort |
Tomova, Iva |
title |
Characterization of heavy metals resistant heterotrophic bacteria from soils in the Windmill Islands region, Wilkes Land, East Antarctica |
title_short |
Characterization of heavy metals resistant heterotrophic bacteria from soils in the Windmill Islands region, Wilkes Land, East Antarctica |
title_full |
Characterization of heavy metals resistant heterotrophic bacteria from soils in the Windmill Islands region, Wilkes Land, East Antarctica |
title_fullStr |
Characterization of heavy metals resistant heterotrophic bacteria from soils in the Windmill Islands region, Wilkes Land, East Antarctica |
title_full_unstemmed |
Characterization of heavy metals resistant heterotrophic bacteria from soils in the Windmill Islands region, Wilkes Land, East Antarctica |
title_sort |
characterization of heavy metals resistant heterotrophic bacteria from soils in the windmill islands region, wilkes land, east antarctica |
publisher |
Walter de Gruyter GmbH |
publishDate |
2014 |
url |
http://dx.doi.org/10.2478/popore-2014-0028 http://content.sciendo.com/view/journals/popore/35/4/article-p593.xml https://www.degruyter.com/view/j/popore.2014.35.issue-4/popore-2014-0028/popore-2014-0028.pdf |
long_lat |
ENVELOPE(120.000,120.000,-69.000,-69.000) ENVELOPE(110.417,110.417,-66.350,-66.350) |
geographic |
Antarctic The Antarctic East Antarctica Wilkes Land Windmill Islands |
geographic_facet |
Antarctic The Antarctic East Antarctica Wilkes Land Windmill Islands |
genre |
Antarc* Antarctic Antarctica East Antarctica Polar Research Wilkes Land Windmill Islands |
genre_facet |
Antarc* Antarctic Antarctica East Antarctica Polar Research Wilkes Land Windmill Islands |
op_source |
Polish Polar Research volume 35, issue 4, page 593-607 ISSN 2081-8262 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/3.0/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.2478/popore-2014-0028 |
container_title |
Polish Polar Research |
container_volume |
35 |
container_issue |
4 |
container_start_page |
593 |
op_container_end_page |
607 |
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1766284596057473024 |