Extracellular hydrolytic enzyme production by proteolytic bacteria from the Antarctic
Abstract Cold−adapted marine bacteria producing extracellular hydrolytic enzymes are important for their industrial application and play a key role in degradation of particulate organic matter in their natural environment. In this work, members of a previously−obtained protease−producing bacterial c...
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Walter de Gruyter GmbH
2013
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crdegruyter:10.2478/popore-2013-0014 2023-05-15T14:07:18+02:00 Extracellular hydrolytic enzyme production by proteolytic bacteria from the Antarctic Tropeano, Mauro Vázquez, Susana Coria, Silvia Turjanski, Adrián Cicero, Daniel Bercovich, Andrés Mac Cormack, Walter 2013 http://dx.doi.org/10.2478/popore-2013-0014 http://content.sciendo.com/view/journals/popore/34/3/article-p253.xml https://www.degruyter.com/view/j/popore.2013.34.issue-3/popore-2013-0014/popore-2013-0014.pdf unknown Walter de Gruyter GmbH Polish Polar Research volume 34, issue 3, page 253-267 ISSN 2081-8262 0138-0338 Ecology Ecology, Evolution, Behavior and Systematics journal-article 2013 crdegruyter https://doi.org/10.2478/popore-2013-0014 2022-05-11T14:46:02Z Abstract Cold−adapted marine bacteria producing extracellular hydrolytic enzymes are important for their industrial application and play a key role in degradation of particulate organic matter in their natural environment. In this work, members of a previously−obtained protease−producing bacterial collection isolated from different marine sources from Potter Cove (King George Island, South Shetlands) were taxonomically identified and screened for their ability to produce other economically relevant enzymes. Eighty−eight proteolytic bacterial isolates were grouped into 25 phylotypes based on their Amplified Ribosomal DNA Restriction Analysis profiles. The sequencing of the 16S rRNA genes from representative isolates of the phylotypes showed that the predominant culturable protease−producing bacteria belonged to the class Gammaproteobacteria and were affiliated to the genera Pseudomonas, Shewanella, Colwellia, and Pseudoalteromonas, the latter being the predominant group (64% of isolates). In addition, members of the classes Actinobacteria, Bacilli and Flavobacteria were found. Among the 88 isolates screened we detected producers of amylases (21), pectinases (67), cellulases (53), CM−cellulases (68), xylanases (55) and agarases (57). More than 85% of the isolates showed at least one of the extracellular enzymatic activities tested, with some of them producing up to six extracellular enzymes. Our results confirmed that using selective conditions to isolate producers of one extracellular enzyme activity increases the probability of recovering bacteria that will also produce additional extracellular enzymes. This finding establishes a starting point for future programs oriented to the prospecting for biomolecules in Antarctica. Article in Journal/Newspaper Antarc* Antarctic Antarctica King George Island Polar Research De Gruyter (via Crossref) Antarctic The Antarctic King George Island Potter Cove Polish Polar Research 34 3 253 267 |
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Open Polar |
collection |
De Gruyter (via Crossref) |
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unknown |
topic |
Ecology Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Ecology Ecology, Evolution, Behavior and Systematics Tropeano, Mauro Vázquez, Susana Coria, Silvia Turjanski, Adrián Cicero, Daniel Bercovich, Andrés Mac Cormack, Walter Extracellular hydrolytic enzyme production by proteolytic bacteria from the Antarctic |
topic_facet |
Ecology Ecology, Evolution, Behavior and Systematics |
description |
Abstract Cold−adapted marine bacteria producing extracellular hydrolytic enzymes are important for their industrial application and play a key role in degradation of particulate organic matter in their natural environment. In this work, members of a previously−obtained protease−producing bacterial collection isolated from different marine sources from Potter Cove (King George Island, South Shetlands) were taxonomically identified and screened for their ability to produce other economically relevant enzymes. Eighty−eight proteolytic bacterial isolates were grouped into 25 phylotypes based on their Amplified Ribosomal DNA Restriction Analysis profiles. The sequencing of the 16S rRNA genes from representative isolates of the phylotypes showed that the predominant culturable protease−producing bacteria belonged to the class Gammaproteobacteria and were affiliated to the genera Pseudomonas, Shewanella, Colwellia, and Pseudoalteromonas, the latter being the predominant group (64% of isolates). In addition, members of the classes Actinobacteria, Bacilli and Flavobacteria were found. Among the 88 isolates screened we detected producers of amylases (21), pectinases (67), cellulases (53), CM−cellulases (68), xylanases (55) and agarases (57). More than 85% of the isolates showed at least one of the extracellular enzymatic activities tested, with some of them producing up to six extracellular enzymes. Our results confirmed that using selective conditions to isolate producers of one extracellular enzyme activity increases the probability of recovering bacteria that will also produce additional extracellular enzymes. This finding establishes a starting point for future programs oriented to the prospecting for biomolecules in Antarctica. |
format |
Article in Journal/Newspaper |
author |
Tropeano, Mauro Vázquez, Susana Coria, Silvia Turjanski, Adrián Cicero, Daniel Bercovich, Andrés Mac Cormack, Walter |
author_facet |
Tropeano, Mauro Vázquez, Susana Coria, Silvia Turjanski, Adrián Cicero, Daniel Bercovich, Andrés Mac Cormack, Walter |
author_sort |
Tropeano, Mauro |
title |
Extracellular hydrolytic enzyme production by proteolytic bacteria from the Antarctic |
title_short |
Extracellular hydrolytic enzyme production by proteolytic bacteria from the Antarctic |
title_full |
Extracellular hydrolytic enzyme production by proteolytic bacteria from the Antarctic |
title_fullStr |
Extracellular hydrolytic enzyme production by proteolytic bacteria from the Antarctic |
title_full_unstemmed |
Extracellular hydrolytic enzyme production by proteolytic bacteria from the Antarctic |
title_sort |
extracellular hydrolytic enzyme production by proteolytic bacteria from the antarctic |
publisher |
Walter de Gruyter GmbH |
publishDate |
2013 |
url |
http://dx.doi.org/10.2478/popore-2013-0014 http://content.sciendo.com/view/journals/popore/34/3/article-p253.xml https://www.degruyter.com/view/j/popore.2013.34.issue-3/popore-2013-0014/popore-2013-0014.pdf |
geographic |
Antarctic The Antarctic King George Island Potter Cove |
geographic_facet |
Antarctic The Antarctic King George Island Potter Cove |
genre |
Antarc* Antarctic Antarctica King George Island Polar Research |
genre_facet |
Antarc* Antarctic Antarctica King George Island Polar Research |
op_source |
Polish Polar Research volume 34, issue 3, page 253-267 ISSN 2081-8262 0138-0338 |
op_doi |
https://doi.org/10.2478/popore-2013-0014 |
container_title |
Polish Polar Research |
container_volume |
34 |
container_issue |
3 |
container_start_page |
253 |
op_container_end_page |
267 |
_version_ |
1766279205441503232 |