Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles
Abstract The immobilization of lipase B from Candida antarctica (CalB) within micronsized poly- N -Isopropylacrylamide (p-NIPAM) microgel particles with a crosslinker content of 5% is reported. The immobilization of the enzyme was reached by an exchange from polar to organic solvents. After determin...
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crdegruyter:10.1524/zpch.2012.0271 2024-04-28T08:02:24+00:00 Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles Gawlitza, Kornelia Wu, Changzhu Georgieva, Radostina Ansorge-Schumacher, Marion von Klitzing, Regine 2012 http://dx.doi.org/10.1524/zpch.2012.0271 https://www.degruyter.com/document/doi/10.1524/zpch.2012.0271/xml https://www.degruyter.com/document/doi/10.1524/zpch.2012.0271/pdf en eng Walter de Gruyter GmbH Zeitschrift für Physikalische Chemie volume 226, issue 7-8, page 749-759 ISSN 2196-7156 0942-9352 Physical and Theoretical Chemistry journal-article 2012 crdegruyter https://doi.org/10.1524/zpch.2012.0271 2024-04-02T06:57:00Z Abstract The immobilization of lipase B from Candida antarctica (CalB) within micronsized poly- N -Isopropylacrylamide (p-NIPAM) microgel particles with a crosslinker content of 5% is reported. The immobilization of the enzyme was reached by an exchange from polar to organic solvents. After determining the embedded amount of CalB within the polymer network, an enhanced specific activity in n -hexane was obtained. Due to the thermoresponsibility of the polymer particles, the activity reaction was done at 25 ºC and 50 ºC. The results presented show that the reversible collapse of the microgel leads to a decreased activity with increasing temperature. Hence, p-NIPAM microgels display a good opportunity to tailor the activity of CalB. An interesting side effect is that CalB presents a suitable probe to estimate the mesh size of the polymer network, since it penetrates in the unlabeled form but not after labeling with FITC. Article in Journal/Newspaper Antarc* Antarctica De Gruyter Zeitschrift für Physikalische Chemie 226 7-8 749 759 |
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English |
topic |
Physical and Theoretical Chemistry |
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Physical and Theoretical Chemistry Gawlitza, Kornelia Wu, Changzhu Georgieva, Radostina Ansorge-Schumacher, Marion von Klitzing, Regine Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles |
topic_facet |
Physical and Theoretical Chemistry |
description |
Abstract The immobilization of lipase B from Candida antarctica (CalB) within micronsized poly- N -Isopropylacrylamide (p-NIPAM) microgel particles with a crosslinker content of 5% is reported. The immobilization of the enzyme was reached by an exchange from polar to organic solvents. After determining the embedded amount of CalB within the polymer network, an enhanced specific activity in n -hexane was obtained. Due to the thermoresponsibility of the polymer particles, the activity reaction was done at 25 ºC and 50 ºC. The results presented show that the reversible collapse of the microgel leads to a decreased activity with increasing temperature. Hence, p-NIPAM microgels display a good opportunity to tailor the activity of CalB. An interesting side effect is that CalB presents a suitable probe to estimate the mesh size of the polymer network, since it penetrates in the unlabeled form but not after labeling with FITC. |
format |
Article in Journal/Newspaper |
author |
Gawlitza, Kornelia Wu, Changzhu Georgieva, Radostina Ansorge-Schumacher, Marion von Klitzing, Regine |
author_facet |
Gawlitza, Kornelia Wu, Changzhu Georgieva, Radostina Ansorge-Schumacher, Marion von Klitzing, Regine |
author_sort |
Gawlitza, Kornelia |
title |
Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles |
title_short |
Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles |
title_full |
Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles |
title_fullStr |
Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles |
title_full_unstemmed |
Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles |
title_sort |
temperature controlled activity of lipase b from candida antarctica after immobilization within p-nipam microgel particles |
publisher |
Walter de Gruyter GmbH |
publishDate |
2012 |
url |
http://dx.doi.org/10.1524/zpch.2012.0271 https://www.degruyter.com/document/doi/10.1524/zpch.2012.0271/xml https://www.degruyter.com/document/doi/10.1524/zpch.2012.0271/pdf |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Zeitschrift für Physikalische Chemie volume 226, issue 7-8, page 749-759 ISSN 2196-7156 0942-9352 |
op_doi |
https://doi.org/10.1524/zpch.2012.0271 |
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Zeitschrift für Physikalische Chemie |
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226 |
container_issue |
7-8 |
container_start_page |
749 |
op_container_end_page |
759 |
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1797573774050066432 |