In vitro Antioxidant Capacities of Two Benzonaphthoxanthenones: Ohioensins F and G, Isolated from the Antarctic Moss Polytrichastrum alpinum
Antioxidant agents against reactive oxygen species can be used for several cosmetic and medicinal applications. This study’s objective was to evaluate the antioxidant activities of Polytrichastrum alpinum (Hedw.) G. L. Sm. (Polytrichaceae), an Antarctic moss species collected from King George Island...
Published in: | Zeitschrift für Naturforschung C |
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Walter de Gruyter GmbH
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Online Access: | http://dx.doi.org/10.1515/znc-2009-3-408 http://www.degruyter.com/view/j/znc.2009.64.issue-3-4/znc-2009-3-408/znc-2009-3-408.xml https://www.degruyter.com/document/doi/10.1515/znc-2009-3-408/xml https://www.degruyter.com/document/doi/10.1515/znc-2009-3-408/pdf |
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crdegruyter:10.1515/znc-2009-3-408 2024-09-15T17:46:27+00:00 In vitro Antioxidant Capacities of Two Benzonaphthoxanthenones: Ohioensins F and G, Isolated from the Antarctic Moss Polytrichastrum alpinum Bhattarai, Hari Datta Paudel, Babita Lee, Hong Kum Oh, Hyuncheol Yim, Joung Han 2009 http://dx.doi.org/10.1515/znc-2009-3-408 http://www.degruyter.com/view/j/znc.2009.64.issue-3-4/znc-2009-3-408/znc-2009-3-408.xml https://www.degruyter.com/document/doi/10.1515/znc-2009-3-408/xml https://www.degruyter.com/document/doi/10.1515/znc-2009-3-408/pdf en eng Walter de Gruyter GmbH http://creativecommons.org/licenses/by-nc-nd/3.0/ Zeitschrift für Naturforschung C volume 64, issue 3-4, page 197-200 ISSN 1865-7125 0939-5075 journal-article 2009 crdegruyter https://doi.org/10.1515/znc-2009-3-408 2024-07-29T04:10:29Z Antioxidant agents against reactive oxygen species can be used for several cosmetic and medicinal applications. This study’s objective was to evaluate the antioxidant activities of Polytrichastrum alpinum (Hedw.) G. L. Sm. (Polytrichaceae), an Antarctic moss species collected from King George Island (Antarctica). The identifi cation of the moss species was performed on the basis of morphological characteristics and molecular sequencing of the 18S rRNA gene. Two benzonaphthoxanthenones: ohioensins F and G, were isolated from the extract after several chromatographic procedures. The various in vitro antioxidant capacities of a methanolic extract of P. alpinum and the isolated compounds were evaluated by analyzing the scavenging capacities of free radicals of 2,2-azino-bis(3-ethylbenzthiazoline- 6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH), the total phenol assay with Folin-Ciocalteu reagent, the ferric ion (Fe 3+ ) reducing power and the nitric oxide (NO) scavenging activity and compared to those of commercial standards for each assay. The experimental data showed that even the crude extract of P. alpinum exhibited potent antioxidant activity. The antioxidant activity was increased two- to seven-fold for the purified compounds. The antioxidant activities of both purified compounds were found to be more or less the same in all experiments. However, the obtained data showed that the Fe3+ reducing power of the purified compounds and crude methanolic extract was almost the same suggesting the presence of other stronger reducing agents in the methanolic extract which could not be isolated in the present experiment. Therefore, further work on the isolation of these stronger antioxidant agents from this moss specimen of the extreme environment is warranted. Developments of laboratory mass culture techniques are anticipated to achieve bulk production of the active constituents for commercial application. Article in Journal/Newspaper Antarc* Antarctic Antarctica King George Island De Gruyter Zeitschrift für Naturforschung C 64 3-4 197 200 |
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De Gruyter |
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crdegruyter |
language |
English |
description |
Antioxidant agents against reactive oxygen species can be used for several cosmetic and medicinal applications. This study’s objective was to evaluate the antioxidant activities of Polytrichastrum alpinum (Hedw.) G. L. Sm. (Polytrichaceae), an Antarctic moss species collected from King George Island (Antarctica). The identifi cation of the moss species was performed on the basis of morphological characteristics and molecular sequencing of the 18S rRNA gene. Two benzonaphthoxanthenones: ohioensins F and G, were isolated from the extract after several chromatographic procedures. The various in vitro antioxidant capacities of a methanolic extract of P. alpinum and the isolated compounds were evaluated by analyzing the scavenging capacities of free radicals of 2,2-azino-bis(3-ethylbenzthiazoline- 6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH), the total phenol assay with Folin-Ciocalteu reagent, the ferric ion (Fe 3+ ) reducing power and the nitric oxide (NO) scavenging activity and compared to those of commercial standards for each assay. The experimental data showed that even the crude extract of P. alpinum exhibited potent antioxidant activity. The antioxidant activity was increased two- to seven-fold for the purified compounds. The antioxidant activities of both purified compounds were found to be more or less the same in all experiments. However, the obtained data showed that the Fe3+ reducing power of the purified compounds and crude methanolic extract was almost the same suggesting the presence of other stronger reducing agents in the methanolic extract which could not be isolated in the present experiment. Therefore, further work on the isolation of these stronger antioxidant agents from this moss specimen of the extreme environment is warranted. Developments of laboratory mass culture techniques are anticipated to achieve bulk production of the active constituents for commercial application. |
format |
Article in Journal/Newspaper |
author |
Bhattarai, Hari Datta Paudel, Babita Lee, Hong Kum Oh, Hyuncheol Yim, Joung Han |
spellingShingle |
Bhattarai, Hari Datta Paudel, Babita Lee, Hong Kum Oh, Hyuncheol Yim, Joung Han In vitro Antioxidant Capacities of Two Benzonaphthoxanthenones: Ohioensins F and G, Isolated from the Antarctic Moss Polytrichastrum alpinum |
author_facet |
Bhattarai, Hari Datta Paudel, Babita Lee, Hong Kum Oh, Hyuncheol Yim, Joung Han |
author_sort |
Bhattarai, Hari Datta |
title |
In vitro Antioxidant Capacities of Two Benzonaphthoxanthenones: Ohioensins F and G, Isolated from the Antarctic Moss Polytrichastrum alpinum |
title_short |
In vitro Antioxidant Capacities of Two Benzonaphthoxanthenones: Ohioensins F and G, Isolated from the Antarctic Moss Polytrichastrum alpinum |
title_full |
In vitro Antioxidant Capacities of Two Benzonaphthoxanthenones: Ohioensins F and G, Isolated from the Antarctic Moss Polytrichastrum alpinum |
title_fullStr |
In vitro Antioxidant Capacities of Two Benzonaphthoxanthenones: Ohioensins F and G, Isolated from the Antarctic Moss Polytrichastrum alpinum |
title_full_unstemmed |
In vitro Antioxidant Capacities of Two Benzonaphthoxanthenones: Ohioensins F and G, Isolated from the Antarctic Moss Polytrichastrum alpinum |
title_sort |
in vitro antioxidant capacities of two benzonaphthoxanthenones: ohioensins f and g, isolated from the antarctic moss polytrichastrum alpinum |
publisher |
Walter de Gruyter GmbH |
publishDate |
2009 |
url |
http://dx.doi.org/10.1515/znc-2009-3-408 http://www.degruyter.com/view/j/znc.2009.64.issue-3-4/znc-2009-3-408/znc-2009-3-408.xml https://www.degruyter.com/document/doi/10.1515/znc-2009-3-408/xml https://www.degruyter.com/document/doi/10.1515/znc-2009-3-408/pdf |
genre |
Antarc* Antarctic Antarctica King George Island |
genre_facet |
Antarc* Antarctic Antarctica King George Island |
op_source |
Zeitschrift für Naturforschung C volume 64, issue 3-4, page 197-200 ISSN 1865-7125 0939-5075 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/3.0/ |
op_doi |
https://doi.org/10.1515/znc-2009-3-408 |
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Zeitschrift für Naturforschung C |
container_volume |
64 |
container_issue |
3-4 |
container_start_page |
197 |
op_container_end_page |
200 |
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1810494587303624704 |