Fluorescence Spectroscopic and Thermodynamic Studies of Chlorophyll Containing Monolayers and Vesicles. Part I: Mixed Monolayers of Pheophytin A and Lecithin

• Published in: Zeitschrift für Naturforschung C
• Main Authors: Heithier, H., Galla, H.-J., Möhwald, H.
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Walter de Gruyter GmbH 1978
• Subjects: General Biochemistry, Genetics and Molecular Biology; DML
• Online Access: http://dx.doi.org/10.1515/znc-1978-5-613; http://www.degruyter.com/view/j/znc.1978.33.issue-5-6/znc-1978-5-613/znc-1978-5-613.xml; https://www.degruyter.com/document/doi/10.1515/znc-1978-5-613/pdf

Mixed monolayers of pheophytin a and α-ʟ-dimyristoyl lecithin (DML) are investigated on the water surface. The studies gain their special value from the simultaneous measurement of surface pressure and fluorescence intensity as a function of the covered area per molecule. A phase separation in the liquid state of the monolayer is established. Phase 1 exists almost exclusively of pheophytin molecules. Phase 2 exists essentially of DML domains solubilizing pheo­phytin in a concentration of 15 mol%. During the DML main transition the pheophytin solubility decreases to about 2 mol%, the excess pheophytin being precipitated within the surface layer. During the pheophytin main transition an ordered structure below the surface layer is formed. A stabilizing interaction between the pheophytin domains and their environment is observed and discussed. A sharp fluorescence change at a pressure below 0.5 dyn/cm indicates another phase transition. It very probably involves an unwrapping of pheophytin from water molecules.