Non-traditional oils with water-soluble substrate as cell growth booster for the production of mannosylerythritol lipids by Pseudozyma antarctica (ATCC 32657) with their antimicrobial activity
Abstract Among glycolipids, mannosylerythritol lipids (MEL), are mild and environmentally friendly surfactants used in various industrial applications. MELs are produced by biofermentation using non-traditional oils with various water-soluble carbon sources as cell growth booster. This substrate aff...
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crdegruyter:10.1515/tsd-2021-2366 2023-05-15T14:11:21+02:00 Non-traditional oils with water-soluble substrate as cell growth booster for the production of mannosylerythritol lipids by Pseudozyma antarctica (ATCC 32657) with their antimicrobial activity Bhangale, Akash P. Wadekar, Sushant D. Kale, Sandeep B. Mali, Suraj N. Pratap, Amit P. 2022 http://dx.doi.org/10.1515/tsd-2021-2366 https://www.degruyter.com/document/doi/10.1515/tsd-2021-2366/xml https://www.degruyter.com/document/doi/10.1515/tsd-2021-2366/pdf en eng Walter de Gruyter GmbH Tenside Surfactants Detergents volume 59, issue 2, page 122-133 ISSN 0932-3414 2195-8564 Condensed Matter Physics General Chemical Engineering General Chemistry journal-article 2022 crdegruyter https://doi.org/10.1515/tsd-2021-2366 2022-04-14T05:01:13Z Abstract Among glycolipids, mannosylerythritol lipids (MEL), are mild and environmentally friendly surfactants used in various industrial applications. MELs are produced by biofermentation using non-traditional oils with various water-soluble carbon sources as cell growth booster. This substrate affects the production yield and cost of MEL. In this research work, the non-traditional oils jatropha oil, karanja oil and neem oil were used as new substrates along with glucose, glycerol and honey as new water-soluble substrates. All these oils are new feedstocks for the production of MEL using Pseudozyma antarctica (ATCC 32657). Jatropha oil, karanja oil and neem oil with honey as substrates resulted in higher MEL yields of (8.07, 7.75, and 1.86) g/L and better cell growth of (8.07, 7.75, and 1.86) g/L, respectively, than non-traditional oils with glucose and glycerol as substrates. Neem oil gave a lower yield of MEL (1.54 g/L) as well as cell growth (6.06 g/L) compared to jatropha oil and karanja oil (7.03 and 6.17) g/L, respectively. Crude MEL from the fermentation broth was detected by thin-layer chromatography (TLC), Fourier transform infrared spectrommetry (FT-IR), high performance liquid chromatography (HPLC) and proton nuclear magnetic resonance spectroscopy ( 1 H NMR). Purified MEL has been used as an antimicrobial agent in cosmetic products associated with gram-positive and gram-negative bacteria and fungi. Article in Journal/Newspaper Antarc* Antarctica De Gruyter (via Crossref) Tenside Surfactants Detergents 59 2 122 133 |
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English |
topic |
Condensed Matter Physics General Chemical Engineering General Chemistry |
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Condensed Matter Physics General Chemical Engineering General Chemistry Bhangale, Akash P. Wadekar, Sushant D. Kale, Sandeep B. Mali, Suraj N. Pratap, Amit P. Non-traditional oils with water-soluble substrate as cell growth booster for the production of mannosylerythritol lipids by Pseudozyma antarctica (ATCC 32657) with their antimicrobial activity |
topic_facet |
Condensed Matter Physics General Chemical Engineering General Chemistry |
description |
Abstract Among glycolipids, mannosylerythritol lipids (MEL), are mild and environmentally friendly surfactants used in various industrial applications. MELs are produced by biofermentation using non-traditional oils with various water-soluble carbon sources as cell growth booster. This substrate affects the production yield and cost of MEL. In this research work, the non-traditional oils jatropha oil, karanja oil and neem oil were used as new substrates along with glucose, glycerol and honey as new water-soluble substrates. All these oils are new feedstocks for the production of MEL using Pseudozyma antarctica (ATCC 32657). Jatropha oil, karanja oil and neem oil with honey as substrates resulted in higher MEL yields of (8.07, 7.75, and 1.86) g/L and better cell growth of (8.07, 7.75, and 1.86) g/L, respectively, than non-traditional oils with glucose and glycerol as substrates. Neem oil gave a lower yield of MEL (1.54 g/L) as well as cell growth (6.06 g/L) compared to jatropha oil and karanja oil (7.03 and 6.17) g/L, respectively. Crude MEL from the fermentation broth was detected by thin-layer chromatography (TLC), Fourier transform infrared spectrommetry (FT-IR), high performance liquid chromatography (HPLC) and proton nuclear magnetic resonance spectroscopy ( 1 H NMR). Purified MEL has been used as an antimicrobial agent in cosmetic products associated with gram-positive and gram-negative bacteria and fungi. |
format |
Article in Journal/Newspaper |
author |
Bhangale, Akash P. Wadekar, Sushant D. Kale, Sandeep B. Mali, Suraj N. Pratap, Amit P. |
author_facet |
Bhangale, Akash P. Wadekar, Sushant D. Kale, Sandeep B. Mali, Suraj N. Pratap, Amit P. |
author_sort |
Bhangale, Akash P. |
title |
Non-traditional oils with water-soluble substrate as cell growth booster for the production of mannosylerythritol lipids by Pseudozyma antarctica (ATCC 32657) with their antimicrobial activity |
title_short |
Non-traditional oils with water-soluble substrate as cell growth booster for the production of mannosylerythritol lipids by Pseudozyma antarctica (ATCC 32657) with their antimicrobial activity |
title_full |
Non-traditional oils with water-soluble substrate as cell growth booster for the production of mannosylerythritol lipids by Pseudozyma antarctica (ATCC 32657) with their antimicrobial activity |
title_fullStr |
Non-traditional oils with water-soluble substrate as cell growth booster for the production of mannosylerythritol lipids by Pseudozyma antarctica (ATCC 32657) with their antimicrobial activity |
title_full_unstemmed |
Non-traditional oils with water-soluble substrate as cell growth booster for the production of mannosylerythritol lipids by Pseudozyma antarctica (ATCC 32657) with their antimicrobial activity |
title_sort |
non-traditional oils with water-soluble substrate as cell growth booster for the production of mannosylerythritol lipids by pseudozyma antarctica (atcc 32657) with their antimicrobial activity |
publisher |
Walter de Gruyter GmbH |
publishDate |
2022 |
url |
http://dx.doi.org/10.1515/tsd-2021-2366 https://www.degruyter.com/document/doi/10.1515/tsd-2021-2366/xml https://www.degruyter.com/document/doi/10.1515/tsd-2021-2366/pdf |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Tenside Surfactants Detergents volume 59, issue 2, page 122-133 ISSN 0932-3414 2195-8564 |
op_doi |
https://doi.org/10.1515/tsd-2021-2366 |
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Tenside Surfactants Detergents |
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59 |
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2 |
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122 |
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