Primer Note: A New Set of Highly Polymorphic Nuclear Microsatellite Markers for Nothofagus nervosa and Related South American Species
Abstract Nothofagus is the main component of southern South American temperate forests. Overexploitation in the past has led to the loss of 40% of the original distribution range. Genetic diversity as well as biological processes shaping the distribution of the genetic variation (e.g. gene flow) con...
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crdegruyter:10.1515/sg-2008-0013 2024-05-19T07:32:37+00:00 Primer Note: A New Set of Highly Polymorphic Nuclear Microsatellite Markers for Nothofagus nervosa and Related South American Species Marchelli, P. Caron, H. Azpilicueta, M. M. Gallo, L. A. 2008 http://dx.doi.org/10.1515/sg-2008-0013 http://content.sciendo.com/view/journals/sg/57/1-6/article-p82.xml https://www.sciendo.com/article/10.1515/sg-2008-0013 en eng Walter de Gruyter GmbH http://creativecommons.org/licenses/by-nc-nd/4.0 Silvae Genetica volume 57, issue 1-6, page 82-85 ISSN 2509-8934 journal-article 2008 crdegruyter https://doi.org/10.1515/sg-2008-0013 2024-05-02T06:51:34Z Abstract Nothofagus is the main component of southern South American temperate forests. Overexploitation in the past has led to the loss of 40% of the original distribution range. Genetic diversity as well as biological processes shaping the distribution of the genetic variation (e.g. gene flow) constitutes basic knowledge for the implementation of conservation measures and for the definition of Evolutionary Significant Units. Nuclear microsatellites are the marker of choice for gene flow and fine-scale genetic structure studies. We enlarged a previous set of microsatellites (SSRs) for South American Nothofagus species, with special concern to Nothofagus nervosa (Phil.) Dim. et Mil. Five new SSRs are presented with allele numbers up to 12 in a single population. The primers transferred well to five related species (N. obliqua (Mirb.) Oerst, N. glauca (Phil.) Krasser, N. dombeyi (Mirb.) Oerst , N. pumilio (Poepp et Endl.) Krasser and N. antarctica (G. Forster) Oerst, with allele numbers up to 11. The high level of polymorphism promises a sufficient power for gene flow and parentage analyses. Article in Journal/Newspaper Antarc* Antarctica De Gruyter Silvae Genetica 57 1-6 82 85 |
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language |
English |
description |
Abstract Nothofagus is the main component of southern South American temperate forests. Overexploitation in the past has led to the loss of 40% of the original distribution range. Genetic diversity as well as biological processes shaping the distribution of the genetic variation (e.g. gene flow) constitutes basic knowledge for the implementation of conservation measures and for the definition of Evolutionary Significant Units. Nuclear microsatellites are the marker of choice for gene flow and fine-scale genetic structure studies. We enlarged a previous set of microsatellites (SSRs) for South American Nothofagus species, with special concern to Nothofagus nervosa (Phil.) Dim. et Mil. Five new SSRs are presented with allele numbers up to 12 in a single population. The primers transferred well to five related species (N. obliqua (Mirb.) Oerst, N. glauca (Phil.) Krasser, N. dombeyi (Mirb.) Oerst , N. pumilio (Poepp et Endl.) Krasser and N. antarctica (G. Forster) Oerst, with allele numbers up to 11. The high level of polymorphism promises a sufficient power for gene flow and parentage analyses. |
format |
Article in Journal/Newspaper |
author |
Marchelli, P. Caron, H. Azpilicueta, M. M. Gallo, L. A. |
spellingShingle |
Marchelli, P. Caron, H. Azpilicueta, M. M. Gallo, L. A. Primer Note: A New Set of Highly Polymorphic Nuclear Microsatellite Markers for Nothofagus nervosa and Related South American Species |
author_facet |
Marchelli, P. Caron, H. Azpilicueta, M. M. Gallo, L. A. |
author_sort |
Marchelli, P. |
title |
Primer Note: A New Set of Highly Polymorphic Nuclear Microsatellite Markers for Nothofagus nervosa and Related South American Species |
title_short |
Primer Note: A New Set of Highly Polymorphic Nuclear Microsatellite Markers for Nothofagus nervosa and Related South American Species |
title_full |
Primer Note: A New Set of Highly Polymorphic Nuclear Microsatellite Markers for Nothofagus nervosa and Related South American Species |
title_fullStr |
Primer Note: A New Set of Highly Polymorphic Nuclear Microsatellite Markers for Nothofagus nervosa and Related South American Species |
title_full_unstemmed |
Primer Note: A New Set of Highly Polymorphic Nuclear Microsatellite Markers for Nothofagus nervosa and Related South American Species |
title_sort |
primer note: a new set of highly polymorphic nuclear microsatellite markers for nothofagus nervosa and related south american species |
publisher |
Walter de Gruyter GmbH |
publishDate |
2008 |
url |
http://dx.doi.org/10.1515/sg-2008-0013 http://content.sciendo.com/view/journals/sg/57/1-6/article-p82.xml https://www.sciendo.com/article/10.1515/sg-2008-0013 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Silvae Genetica volume 57, issue 1-6, page 82-85 ISSN 2509-8934 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/4.0 |
op_doi |
https://doi.org/10.1515/sg-2008-0013 |
container_title |
Silvae Genetica |
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57 |
container_issue |
1-6 |
container_start_page |
82 |
op_container_end_page |
85 |
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1799470725517541376 |