Isolation and characterization of an Antarctic Flavobacterium strain with agarase and alginate lyase activities
Abstract Several bacteria that are associated with macroalgae can use phycocolloids as a carbon source. Strain INACH002, isolated from decomposing Porphyra (Rhodophyta), in King George Island, Antarctica, was screened and characterized for the ability to produce agarase and alginate-lyase enzymatic...
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crdegruyter:10.1515/popore-2016-0021 2024-09-09T19:04:41+00:00 Isolation and characterization of an Antarctic Flavobacterium strain with agarase and alginate lyase activities Lavín, Paris Atala, Cristian Gallardo-Cerda, Jorge Gonzalez-Aravena, Marcelo De La Iglesia, Rodrigo Oses, Rómulo Torres-Díaz, Cristian Trefault, Nicole Molina-Montenegro, Marco A. Laughinghouse IV, H. Dail 2016 http://dx.doi.org/10.1515/popore-2016-0021 http://content.sciendo.com/view/journals/popore/37/3/article-p403.xml https://www.degruyter.com/view/j/popore.2016.37.issue-3/popore-2016-0021/popore-2016-0021.pdf unknown Walter de Gruyter GmbH http://creativecommons.org/licenses/by-nc-nd/4.0 Polish Polar Research volume 37, issue 3, page 403-419 ISSN 2081-8262 journal-article 2016 crdegruyter https://doi.org/10.1515/popore-2016-0021 2024-08-05T04:13:42Z Abstract Several bacteria that are associated with macroalgae can use phycocolloids as a carbon source. Strain INACH002, isolated from decomposing Porphyra (Rhodophyta), in King George Island, Antarctica, was screened and characterized for the ability to produce agarase and alginate-lyase enzymatic activities. Our strain INACH002 was identified as a member of the genus Flavobacterium, closely related to Flavobacterium faecale, using 16S rRNA gene analysis. The INACH002 strain was characterized as psychrotrophic due to its optimal temperature (17ºC) and maximum temperature (20°C) of growth. Agarase and alginate-lyase displayed enzymatic activities within a range of 10°C to 50°C, with differences in the optimal temperature to hydrolyze agar (50°C), agarose (50°C) and alginate (30°C) during the first 30 min of activity. Strain Flavobacterium INACH002 is a promising Antarctic biotechnological resource; however, further research is required to illustrate the structural and functional bases of the enzymatic performance observed during the degradation of different substrates at different temperatures. Article in Journal/Newspaper Antarc* Antarctic Antarctica King George Island Polar Research De Gruyter Antarctic King George Island Polish Polar Research 37 3 403 419 |
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Abstract Several bacteria that are associated with macroalgae can use phycocolloids as a carbon source. Strain INACH002, isolated from decomposing Porphyra (Rhodophyta), in King George Island, Antarctica, was screened and characterized for the ability to produce agarase and alginate-lyase enzymatic activities. Our strain INACH002 was identified as a member of the genus Flavobacterium, closely related to Flavobacterium faecale, using 16S rRNA gene analysis. The INACH002 strain was characterized as psychrotrophic due to its optimal temperature (17ºC) and maximum temperature (20°C) of growth. Agarase and alginate-lyase displayed enzymatic activities within a range of 10°C to 50°C, with differences in the optimal temperature to hydrolyze agar (50°C), agarose (50°C) and alginate (30°C) during the first 30 min of activity. Strain Flavobacterium INACH002 is a promising Antarctic biotechnological resource; however, further research is required to illustrate the structural and functional bases of the enzymatic performance observed during the degradation of different substrates at different temperatures. |
format |
Article in Journal/Newspaper |
author |
Lavín, Paris Atala, Cristian Gallardo-Cerda, Jorge Gonzalez-Aravena, Marcelo De La Iglesia, Rodrigo Oses, Rómulo Torres-Díaz, Cristian Trefault, Nicole Molina-Montenegro, Marco A. Laughinghouse IV, H. Dail |
spellingShingle |
Lavín, Paris Atala, Cristian Gallardo-Cerda, Jorge Gonzalez-Aravena, Marcelo De La Iglesia, Rodrigo Oses, Rómulo Torres-Díaz, Cristian Trefault, Nicole Molina-Montenegro, Marco A. Laughinghouse IV, H. Dail Isolation and characterization of an Antarctic Flavobacterium strain with agarase and alginate lyase activities |
author_facet |
Lavín, Paris Atala, Cristian Gallardo-Cerda, Jorge Gonzalez-Aravena, Marcelo De La Iglesia, Rodrigo Oses, Rómulo Torres-Díaz, Cristian Trefault, Nicole Molina-Montenegro, Marco A. Laughinghouse IV, H. Dail |
author_sort |
Lavín, Paris |
title |
Isolation and characterization of an Antarctic Flavobacterium strain with agarase and alginate lyase activities |
title_short |
Isolation and characterization of an Antarctic Flavobacterium strain with agarase and alginate lyase activities |
title_full |
Isolation and characterization of an Antarctic Flavobacterium strain with agarase and alginate lyase activities |
title_fullStr |
Isolation and characterization of an Antarctic Flavobacterium strain with agarase and alginate lyase activities |
title_full_unstemmed |
Isolation and characterization of an Antarctic Flavobacterium strain with agarase and alginate lyase activities |
title_sort |
isolation and characterization of an antarctic flavobacterium strain with agarase and alginate lyase activities |
publisher |
Walter de Gruyter GmbH |
publishDate |
2016 |
url |
http://dx.doi.org/10.1515/popore-2016-0021 http://content.sciendo.com/view/journals/popore/37/3/article-p403.xml https://www.degruyter.com/view/j/popore.2016.37.issue-3/popore-2016-0021/popore-2016-0021.pdf |
geographic |
Antarctic King George Island |
geographic_facet |
Antarctic King George Island |
genre |
Antarc* Antarctic Antarctica King George Island Polar Research |
genre_facet |
Antarc* Antarctic Antarctica King George Island Polar Research |
op_source |
Polish Polar Research volume 37, issue 3, page 403-419 ISSN 2081-8262 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/4.0 |
op_doi |
https://doi.org/10.1515/popore-2016-0021 |
container_title |
Polish Polar Research |
container_volume |
37 |
container_issue |
3 |
container_start_page |
403 |
op_container_end_page |
419 |
_version_ |
1809818707023626240 |