Complement activation by salivary agglutinin is secretor status dependent
Abstract After mucosal damage or gingival inflammation, complement proteins leak into the oral cavity and mix with salivary proteins such as salivary agglutinin (SAG/gp-340/DMBT1). This protein is encoded by the gene Deleted in Malignant Brain Tumors 1 ( DMBT1 ), and it aggregates bacteria, viruses...
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Walter de Gruyter GmbH
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crdegruyter:10.1515/hsz-2014-0200 2023-05-15T13:27:51+02:00 Complement activation by salivary agglutinin is secretor status dependent Gunput, Sabrina T.G. Ligtenberg, Antoon J.M. Terlouw, Bas Brouwer, Mieke Veerman, Enno C.I. Wouters, Diana 2014 http://dx.doi.org/10.1515/hsz-2014-0200 http://www.degruyter.com/view/j/bchm.2015.396.issue-1/hsz-2014-0200/hsz-2014-0200.xml https://www.degruyter.com/document/doi/10.1515/hsz-2014-0200/xml https://www.degruyter.com/document/doi/10.1515/hsz-2014-0200/pdf en eng Walter de Gruyter GmbH Biological Chemistry volume 396, issue 1, page 35-43 ISSN 1437-4315 1431-6730 Clinical Biochemistry Molecular Biology Biochemistry journal-article 2014 crdegruyter https://doi.org/10.1515/hsz-2014-0200 2022-06-16T13:41:10Z Abstract After mucosal damage or gingival inflammation, complement proteins leak into the oral cavity and mix with salivary proteins such as salivary agglutinin (SAG/gp-340/DMBT1). This protein is encoded by the gene Deleted in Malignant Brain Tumors 1 ( DMBT1 ), and it aggregates bacteria, viruses and fungi, and activates the lectin pathway of the complement system. In the lectin pathway, carbohydrate structures on pathogens or altered self cells are recognized. SAG is highly glycosylated, partly on the basis of the donor’s blood group status. Whereas secretors express Lewis b, Lewis y, and antigens from the ABO-blood group system on SAG, non-secretors do not. Through mannose-binding lectin (MBL) binding and C4 deposition assays, we aimed to identify the chemical structures on SAG that are responsible for complement activation. The complement-activating properties of SAG were completely abolished by oxidation of its carbohydrate moiety. SAG-mediated activation of complement was also inhibited in the presence of saccharides such as fucose and Lewis b carbohydrates, and also after pretreatment with the fucose-binding lectin, Anguilla anguilla agglutinin. Complement activation was significantly ( p <0.01) higher in secretors than in non-secretors. Our results suggest that fucose-rich oligosaccharide sidechains, such as Lewis b antigens, are involved in the activation of complement by SAG. Article in Journal/Newspaper Anguilla anguilla De Gruyter (via Crossref) Biological Chemistry 396 1 35 43 |
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De Gruyter (via Crossref) |
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English |
topic |
Clinical Biochemistry Molecular Biology Biochemistry |
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Clinical Biochemistry Molecular Biology Biochemistry Gunput, Sabrina T.G. Ligtenberg, Antoon J.M. Terlouw, Bas Brouwer, Mieke Veerman, Enno C.I. Wouters, Diana Complement activation by salivary agglutinin is secretor status dependent |
topic_facet |
Clinical Biochemistry Molecular Biology Biochemistry |
description |
Abstract After mucosal damage or gingival inflammation, complement proteins leak into the oral cavity and mix with salivary proteins such as salivary agglutinin (SAG/gp-340/DMBT1). This protein is encoded by the gene Deleted in Malignant Brain Tumors 1 ( DMBT1 ), and it aggregates bacteria, viruses and fungi, and activates the lectin pathway of the complement system. In the lectin pathway, carbohydrate structures on pathogens or altered self cells are recognized. SAG is highly glycosylated, partly on the basis of the donor’s blood group status. Whereas secretors express Lewis b, Lewis y, and antigens from the ABO-blood group system on SAG, non-secretors do not. Through mannose-binding lectin (MBL) binding and C4 deposition assays, we aimed to identify the chemical structures on SAG that are responsible for complement activation. The complement-activating properties of SAG were completely abolished by oxidation of its carbohydrate moiety. SAG-mediated activation of complement was also inhibited in the presence of saccharides such as fucose and Lewis b carbohydrates, and also after pretreatment with the fucose-binding lectin, Anguilla anguilla agglutinin. Complement activation was significantly ( p <0.01) higher in secretors than in non-secretors. Our results suggest that fucose-rich oligosaccharide sidechains, such as Lewis b antigens, are involved in the activation of complement by SAG. |
format |
Article in Journal/Newspaper |
author |
Gunput, Sabrina T.G. Ligtenberg, Antoon J.M. Terlouw, Bas Brouwer, Mieke Veerman, Enno C.I. Wouters, Diana |
author_facet |
Gunput, Sabrina T.G. Ligtenberg, Antoon J.M. Terlouw, Bas Brouwer, Mieke Veerman, Enno C.I. Wouters, Diana |
author_sort |
Gunput, Sabrina T.G. |
title |
Complement activation by salivary agglutinin is secretor status dependent |
title_short |
Complement activation by salivary agglutinin is secretor status dependent |
title_full |
Complement activation by salivary agglutinin is secretor status dependent |
title_fullStr |
Complement activation by salivary agglutinin is secretor status dependent |
title_full_unstemmed |
Complement activation by salivary agglutinin is secretor status dependent |
title_sort |
complement activation by salivary agglutinin is secretor status dependent |
publisher |
Walter de Gruyter GmbH |
publishDate |
2014 |
url |
http://dx.doi.org/10.1515/hsz-2014-0200 http://www.degruyter.com/view/j/bchm.2015.396.issue-1/hsz-2014-0200/hsz-2014-0200.xml https://www.degruyter.com/document/doi/10.1515/hsz-2014-0200/xml https://www.degruyter.com/document/doi/10.1515/hsz-2014-0200/pdf |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Biological Chemistry volume 396, issue 1, page 35-43 ISSN 1437-4315 1431-6730 |
op_doi |
https://doi.org/10.1515/hsz-2014-0200 |
container_title |
Biological Chemistry |
container_volume |
396 |
container_issue |
1 |
container_start_page |
35 |
op_container_end_page |
43 |
_version_ |
1766400720882368512 |