Tlr2, Tjp1 genes expression during wound healing dynamics – with melanin treatment
Abstract Wound healing is the complex and dynamic process of replacing devitalized and missing cellular structures and tissue layers. We have previously shown that melanin, herein, produced by the Antarctic black yeast fungi Pseudonadsoniella brunea (Nadsoniella nigra sp. X-1), has expressed a cyto-...
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crdegruyter:10.1515/cipms-2017-0016 2023-05-15T14:13:36+02:00 Tlr2, Tjp1 genes expression during wound healing dynamics – with melanin treatment Dranitsina, Alevtina Dvorshchenko, Kateryna Taburets, Olesya Grebinyk, Dmytro Ostapchenko, Ludmila Beregova, Tetyana 2017 http://dx.doi.org/10.1515/cipms-2017-0016 http://content.sciendo.com/view/journals/cipms/30/2/article-p81.xml https://www.sciendo.com/article/10.1515/cipms-2017-0016 en eng Walter de Gruyter GmbH http://creativecommons.org/licenses/by-nc-nd/4.0 CC-BY-NC-ND Current Issues in Pharmacy and Medical Sciences volume 30, issue 2, page 81-85 ISSN 2300-6676 2084-980X Pharmacology Molecular Biology General Medicine Biochemistry journal-article 2017 crdegruyter https://doi.org/10.1515/cipms-2017-0016 2022-04-14T05:06:05Z Abstract Wound healing is the complex and dynamic process of replacing devitalized and missing cellular structures and tissue layers. We have previously shown that melanin, herein, produced by the Antarctic black yeast fungi Pseudonadsoniella brunea (Nadsoniella nigra sp. X-1), has expressed a cyto-protective effect, promoted rapid wound healing of various ethiology and can be offered as a new dermatropic drug. The current study was conducted on a rat model of purulent necrotic wound. In each model, one group was a control, while in the others, wound healing occurred without drug application or with administration of 0,5% carbopol or with both 0,5% carbopol and 0,1% melanin. The pro-oxidant-antioxidant balance in skin gomogenate in dynamics on 3, 6, 9, 14 and day of full epithelization was estimated using the spectrophotometric biochemical method. Moreover, so as to understand the role played by the Tlr2 and Tjp1 in the process of wound healing and scar formation, Tlr2, Tjp1gene expression and genetic mRNA was determined with quantitative RT-PCR. The application of our pharmacological composition stimulated the decrease of Tlr2 and Tjp1 gene expression against the background of suppression of free radical processes (reduction of superoxide anion radical content) with epithelization and without scarring. The results of this study have shown the positive effects of melanin on wound healing. The obtained results indicate the advisability of applying melanin for the treatment of inflammatory processes. Article in Journal/Newspaper Antarc* Antarctic De Gruyter (via Crossref) Antarctic The Antarctic Current Issues in Pharmacy and Medical Sciences 30 2 81 85 |
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Open Polar |
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De Gruyter (via Crossref) |
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English |
topic |
Pharmacology Molecular Biology General Medicine Biochemistry |
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Pharmacology Molecular Biology General Medicine Biochemistry Dranitsina, Alevtina Dvorshchenko, Kateryna Taburets, Olesya Grebinyk, Dmytro Ostapchenko, Ludmila Beregova, Tetyana Tlr2, Tjp1 genes expression during wound healing dynamics – with melanin treatment |
topic_facet |
Pharmacology Molecular Biology General Medicine Biochemistry |
description |
Abstract Wound healing is the complex and dynamic process of replacing devitalized and missing cellular structures and tissue layers. We have previously shown that melanin, herein, produced by the Antarctic black yeast fungi Pseudonadsoniella brunea (Nadsoniella nigra sp. X-1), has expressed a cyto-protective effect, promoted rapid wound healing of various ethiology and can be offered as a new dermatropic drug. The current study was conducted on a rat model of purulent necrotic wound. In each model, one group was a control, while in the others, wound healing occurred without drug application or with administration of 0,5% carbopol or with both 0,5% carbopol and 0,1% melanin. The pro-oxidant-antioxidant balance in skin gomogenate in dynamics on 3, 6, 9, 14 and day of full epithelization was estimated using the spectrophotometric biochemical method. Moreover, so as to understand the role played by the Tlr2 and Tjp1 in the process of wound healing and scar formation, Tlr2, Tjp1gene expression and genetic mRNA was determined with quantitative RT-PCR. The application of our pharmacological composition stimulated the decrease of Tlr2 and Tjp1 gene expression against the background of suppression of free radical processes (reduction of superoxide anion radical content) with epithelization and without scarring. The results of this study have shown the positive effects of melanin on wound healing. The obtained results indicate the advisability of applying melanin for the treatment of inflammatory processes. |
format |
Article in Journal/Newspaper |
author |
Dranitsina, Alevtina Dvorshchenko, Kateryna Taburets, Olesya Grebinyk, Dmytro Ostapchenko, Ludmila Beregova, Tetyana |
author_facet |
Dranitsina, Alevtina Dvorshchenko, Kateryna Taburets, Olesya Grebinyk, Dmytro Ostapchenko, Ludmila Beregova, Tetyana |
author_sort |
Dranitsina, Alevtina |
title |
Tlr2, Tjp1 genes expression during wound healing dynamics – with melanin treatment |
title_short |
Tlr2, Tjp1 genes expression during wound healing dynamics – with melanin treatment |
title_full |
Tlr2, Tjp1 genes expression during wound healing dynamics – with melanin treatment |
title_fullStr |
Tlr2, Tjp1 genes expression during wound healing dynamics – with melanin treatment |
title_full_unstemmed |
Tlr2, Tjp1 genes expression during wound healing dynamics – with melanin treatment |
title_sort |
tlr2, tjp1 genes expression during wound healing dynamics – with melanin treatment |
publisher |
Walter de Gruyter GmbH |
publishDate |
2017 |
url |
http://dx.doi.org/10.1515/cipms-2017-0016 http://content.sciendo.com/view/journals/cipms/30/2/article-p81.xml https://www.sciendo.com/article/10.1515/cipms-2017-0016 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Current Issues in Pharmacy and Medical Sciences volume 30, issue 2, page 81-85 ISSN 2300-6676 2084-980X |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/4.0 |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1515/cipms-2017-0016 |
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Current Issues in Pharmacy and Medical Sciences |
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30 |
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2 |
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81 |
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85 |
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