Superoxide dismutase and ascorbate peroxidase genes in Antarctic endemic brown alga Ascoseira mirabilis (Ascoseirales, Phaeophyceae): data mining of a de novo transcriptome
Abstract The Antarctic endemic brown alga Ascoseira mirabilis is physically and physiologically well adapted to the extreme polar environment. To better understand the molecular strategies associated with stress adaptation, the transcriptome of A. mirabilis was sequenced, and its antioxidant enzyme...
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crdegruyter:10.1515/bot-2020-0025 2024-09-09T19:08:46+00:00 Superoxide dismutase and ascorbate peroxidase genes in Antarctic endemic brown alga Ascoseira mirabilis (Ascoseirales, Phaeophyceae): data mining of a de novo transcriptome Liu, Chenlin Wang, Xiuliang Chinese National Antarctic Research Exploration National Key R&D programme of China 2020 http://dx.doi.org/10.1515/bot-2020-0025 https://www.degruyter.com/view/journals/botm/63/6/article-p541.xml https://www.degruyter.com/document/doi/10.1515/bot-2020-0025/xml https://www.degruyter.com/document/doi/10.1515/bot-2020-0025/pdf en eng Walter de Gruyter GmbH Botanica Marina volume 63, issue 6, page 541-549 ISSN 1437-4323 0006-8055 journal-article 2020 crdegruyter https://doi.org/10.1515/bot-2020-0025 2024-07-29T04:11:24Z Abstract The Antarctic endemic brown alga Ascoseira mirabilis is physically and physiologically well adapted to the extreme polar environment. To better understand the molecular strategies associated with stress adaptation, the transcriptome of A. mirabilis was sequenced, and its antioxidant enzyme genes were identified and compared with other algae. A total of 126,576 unigenes with a mean length of 734 bp and N50 of 1174 bp was assembled from the transcriptome data. Phylogenetic analysis revealed that dramatic evolutionary changes had occurred in the brown algal superoxide dismutases (SODs) and class I peroxidases that were not consistent with the phylogeny of the species. Fe/Mn SODs are more prevalent in brown algae than in red and green algae. Two additional Fe-SODs in A. mirabilis were phylogenetically closely related to those of green algae, but not to those in other brown algae. We also identified three A. mirabilis ascorbate peroxidase (APx) genes that had a different origin from other brown algal APxs. The SOD and APx genes specifically identified in A. mirabilis will be crucial for understanding the evolution of the algal antioxidant enzymes that contribute to ecological success under extreme environmental conditions in the Antarctic region. Article in Journal/Newspaper Antarc* Antarctic De Gruyter Antarctic The Antarctic Botanica Marina 63 6 541 549 |
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De Gruyter |
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English |
description |
Abstract The Antarctic endemic brown alga Ascoseira mirabilis is physically and physiologically well adapted to the extreme polar environment. To better understand the molecular strategies associated with stress adaptation, the transcriptome of A. mirabilis was sequenced, and its antioxidant enzyme genes were identified and compared with other algae. A total of 126,576 unigenes with a mean length of 734 bp and N50 of 1174 bp was assembled from the transcriptome data. Phylogenetic analysis revealed that dramatic evolutionary changes had occurred in the brown algal superoxide dismutases (SODs) and class I peroxidases that were not consistent with the phylogeny of the species. Fe/Mn SODs are more prevalent in brown algae than in red and green algae. Two additional Fe-SODs in A. mirabilis were phylogenetically closely related to those of green algae, but not to those in other brown algae. We also identified three A. mirabilis ascorbate peroxidase (APx) genes that had a different origin from other brown algal APxs. The SOD and APx genes specifically identified in A. mirabilis will be crucial for understanding the evolution of the algal antioxidant enzymes that contribute to ecological success under extreme environmental conditions in the Antarctic region. |
author2 |
Chinese National Antarctic Research Exploration National Key R&D programme of China |
format |
Article in Journal/Newspaper |
author |
Liu, Chenlin Wang, Xiuliang |
spellingShingle |
Liu, Chenlin Wang, Xiuliang Superoxide dismutase and ascorbate peroxidase genes in Antarctic endemic brown alga Ascoseira mirabilis (Ascoseirales, Phaeophyceae): data mining of a de novo transcriptome |
author_facet |
Liu, Chenlin Wang, Xiuliang |
author_sort |
Liu, Chenlin |
title |
Superoxide dismutase and ascorbate peroxidase genes in Antarctic endemic brown alga Ascoseira mirabilis (Ascoseirales, Phaeophyceae): data mining of a de novo transcriptome |
title_short |
Superoxide dismutase and ascorbate peroxidase genes in Antarctic endemic brown alga Ascoseira mirabilis (Ascoseirales, Phaeophyceae): data mining of a de novo transcriptome |
title_full |
Superoxide dismutase and ascorbate peroxidase genes in Antarctic endemic brown alga Ascoseira mirabilis (Ascoseirales, Phaeophyceae): data mining of a de novo transcriptome |
title_fullStr |
Superoxide dismutase and ascorbate peroxidase genes in Antarctic endemic brown alga Ascoseira mirabilis (Ascoseirales, Phaeophyceae): data mining of a de novo transcriptome |
title_full_unstemmed |
Superoxide dismutase and ascorbate peroxidase genes in Antarctic endemic brown alga Ascoseira mirabilis (Ascoseirales, Phaeophyceae): data mining of a de novo transcriptome |
title_sort |
superoxide dismutase and ascorbate peroxidase genes in antarctic endemic brown alga ascoseira mirabilis (ascoseirales, phaeophyceae): data mining of a de novo transcriptome |
publisher |
Walter de Gruyter GmbH |
publishDate |
2020 |
url |
http://dx.doi.org/10.1515/bot-2020-0025 https://www.degruyter.com/view/journals/botm/63/6/article-p541.xml https://www.degruyter.com/document/doi/10.1515/bot-2020-0025/xml https://www.degruyter.com/document/doi/10.1515/bot-2020-0025/pdf |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Botanica Marina volume 63, issue 6, page 541-549 ISSN 1437-4323 0006-8055 |
op_doi |
https://doi.org/10.1515/bot-2020-0025 |
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Botanica Marina |
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63 |
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6 |
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541 |
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549 |
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