Thermal adaptation in a marine-derived tropical strain of Fusarium equiseti and polar strains of Pseudogymnoascus spp. under different nutrient sources
Abstract We documented relative growth rates (RGRs) and activities of extracellular hydrolytic enzymes (EHEs) of one marine-derived tropical strain of Fusarium equiseti originally isolated from Malaysia and two polar strains of Pseudogymnoascus spp. from the Arctic and Antarctic under various temper...
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crdegruyter:10.1515/bot-2017-0049 2024-05-19T07:31:07+00:00 Thermal adaptation in a marine-derived tropical strain of Fusarium equiseti and polar strains of Pseudogymnoascus spp. under different nutrient sources Tajuddin, Natasha Rizman-Idid, Mohammed Convey, Peter Alias, Siti Aisyah 2017 http://dx.doi.org/10.1515/bot-2017-0049 http://www.degruyter.com/view/j/botm.2018.61.issue-1/bot-2017-0049/bot-2017-0049.xml https://www.degruyter.com/document/doi/10.1515/bot-2017-0049/xml https://www.degruyter.com/document/doi/10.1515/bot-2017-0049/pdf en eng Walter de Gruyter GmbH Botanica Marina volume 61, issue 1, page 9-20 ISSN 1437-4323 0006-8055 journal-article 2017 crdegruyter https://doi.org/10.1515/bot-2017-0049 2024-05-02T06:51:59Z Abstract We documented relative growth rates (RGRs) and activities of extracellular hydrolytic enzymes (EHEs) of one marine-derived tropical strain of Fusarium equiseti originally isolated from Malaysia and two polar strains of Pseudogymnoascus spp. from the Arctic and Antarctic under various temperatures and different nutrient conditions. RGRs and relative enzyme activities (RAs) of protease, amylase and cellulase were screened in seawater nutrient assay plates augmented with either skim milk, soluble starch or carboxymethylcellulose with trypan blue, respectively, across culture temperatures between 5°C and 40°C. Measures of RGR were fitted into third-degree polynomial and Brière-2 temperature-dependent models to estimate optimum temperatures for growth ( T opt ) and maximum growth rates ( RGR max ), and were used to calculate temperature coefficients ( Q 10 ) and activation energies ( E a ) for growth. All studied strains showed highest RGR and RA when grown using a skim milk nutrient assay. T opt for growth was 25°C in F. equiseti and 20°C in Pseudogymnoascus spp. Only F. equiseti showed cellulase activity. These data suggest a preference for protein-based substrates over plant-derived substrates for metabolism in these fungal strains. The tropical F. equiseti could utilise higher levels of thermal energy for growth than the polar strains of Pseudogymnoascus spp., implying adaptation of these fungi to different bioclimatic regions. Article in Journal/Newspaper Antarc* Antarctic Arctic De Gruyter Botanica Marina 61 1 9 20 |
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English |
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Abstract We documented relative growth rates (RGRs) and activities of extracellular hydrolytic enzymes (EHEs) of one marine-derived tropical strain of Fusarium equiseti originally isolated from Malaysia and two polar strains of Pseudogymnoascus spp. from the Arctic and Antarctic under various temperatures and different nutrient conditions. RGRs and relative enzyme activities (RAs) of protease, amylase and cellulase were screened in seawater nutrient assay plates augmented with either skim milk, soluble starch or carboxymethylcellulose with trypan blue, respectively, across culture temperatures between 5°C and 40°C. Measures of RGR were fitted into third-degree polynomial and Brière-2 temperature-dependent models to estimate optimum temperatures for growth ( T opt ) and maximum growth rates ( RGR max ), and were used to calculate temperature coefficients ( Q 10 ) and activation energies ( E a ) for growth. All studied strains showed highest RGR and RA when grown using a skim milk nutrient assay. T opt for growth was 25°C in F. equiseti and 20°C in Pseudogymnoascus spp. Only F. equiseti showed cellulase activity. These data suggest a preference for protein-based substrates over plant-derived substrates for metabolism in these fungal strains. The tropical F. equiseti could utilise higher levels of thermal energy for growth than the polar strains of Pseudogymnoascus spp., implying adaptation of these fungi to different bioclimatic regions. |
format |
Article in Journal/Newspaper |
author |
Tajuddin, Natasha Rizman-Idid, Mohammed Convey, Peter Alias, Siti Aisyah |
spellingShingle |
Tajuddin, Natasha Rizman-Idid, Mohammed Convey, Peter Alias, Siti Aisyah Thermal adaptation in a marine-derived tropical strain of Fusarium equiseti and polar strains of Pseudogymnoascus spp. under different nutrient sources |
author_facet |
Tajuddin, Natasha Rizman-Idid, Mohammed Convey, Peter Alias, Siti Aisyah |
author_sort |
Tajuddin, Natasha |
title |
Thermal adaptation in a marine-derived tropical strain of Fusarium equiseti and polar strains of Pseudogymnoascus spp. under different nutrient sources |
title_short |
Thermal adaptation in a marine-derived tropical strain of Fusarium equiseti and polar strains of Pseudogymnoascus spp. under different nutrient sources |
title_full |
Thermal adaptation in a marine-derived tropical strain of Fusarium equiseti and polar strains of Pseudogymnoascus spp. under different nutrient sources |
title_fullStr |
Thermal adaptation in a marine-derived tropical strain of Fusarium equiseti and polar strains of Pseudogymnoascus spp. under different nutrient sources |
title_full_unstemmed |
Thermal adaptation in a marine-derived tropical strain of Fusarium equiseti and polar strains of Pseudogymnoascus spp. under different nutrient sources |
title_sort |
thermal adaptation in a marine-derived tropical strain of fusarium equiseti and polar strains of pseudogymnoascus spp. under different nutrient sources |
publisher |
Walter de Gruyter GmbH |
publishDate |
2017 |
url |
http://dx.doi.org/10.1515/bot-2017-0049 http://www.degruyter.com/view/j/botm.2018.61.issue-1/bot-2017-0049/bot-2017-0049.xml https://www.degruyter.com/document/doi/10.1515/bot-2017-0049/xml https://www.degruyter.com/document/doi/10.1515/bot-2017-0049/pdf |
genre |
Antarc* Antarctic Arctic |
genre_facet |
Antarc* Antarctic Arctic |
op_source |
Botanica Marina volume 61, issue 1, page 9-20 ISSN 1437-4323 0006-8055 |
op_doi |
https://doi.org/10.1515/bot-2017-0049 |
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Botanica Marina |
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61 |
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1 |
container_start_page |
9 |
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20 |
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1799468958346117120 |