Taxonomy of Kudoa species (Myxosporea), using a small-subunit ribosomal DNA sequence
Myxosporeans in the genus Kudoa infect the flesh of many marine fishes and often cause unsightly lesions and softening of the flesh texture. We are particularly interested in K. thyrsites because it is associated with soft flesh in the Atlantic salmon (Salmo solar), an important commercial species i...
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1997
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crcansciencepubl:10.1139/z97-846 2024-06-23T07:51:16+00:00 Taxonomy of Kudoa species (Myxosporea), using a small-subunit ribosomal DNA sequence Hervio, D. M. L. Khattra, J. Devlin, R. H. Kent, M. L. Sakanari, J. Yokoyama, H. 1997 http://dx.doi.org/10.1139/z97-846 http://www.nrcresearchpress.com/doi/pdf/10.1139/z97-846 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Zoology volume 75, issue 12, page 2112-2119 ISSN 0008-4301 1480-3283 journal-article 1997 crcansciencepubl https://doi.org/10.1139/z97-846 2024-05-24T13:05:53Z Myxosporeans in the genus Kudoa infect the flesh of many marine fishes and often cause unsightly lesions and softening of the flesh texture. We are particularly interested in K. thyrsites because it is associated with soft flesh in the Atlantic salmon (Salmo solar), an important commercial species in Canada. Sequences of the small-subunit (SSU) rDNA (about 1600 base pairs) were obtained from K. miniauriculata, K. amanuensis, and K. poniformis. We aligned these sequences with one obtained from coho salmon (Oncorhynchus kisutch) and designed "Kudoa general" primers (KUD1f and KUD2r). These primers, in combination with other general primers, were then used to obtain the SSU rDNA sequence of K. thyrsites from two host species, Atlantic salmon and tubesnout (Aulorhynchus flavidus), from British Columbia, Canada. Sequence comparisons of these isolates indicated that Kudoa species cluster by geographic location rather than by morphology of spores. The three species from the eastern Pacific were approximately 97% identical, whereas K. amamiensis (from Japan) was about 91% identical with these species. Sequence comparisons of K. thyrsites from Atlantic salmon and tubesnout revealed a difference of only 0.07% between these isolates. Comparison of SSU rDNA sequences from the four Kudoa species and Henneguya salminicolo analyzed in this study with those from other available myxosporean genera (Myxidium and Myxoholus) showed that taxonomic divisions at the order and suborder levels were consistent with classical views of the taxonomy of the Myxosporea. Using specific regions of the SSU rDNA, we also developed a sensitive and specific polymerase chain reaction test for detection of K. thyrsites. Article in Journal/Newspaper Atlantic salmon Canadian Science Publishing British Columbia ENVELOPE(-125.003,-125.003,54.000,54.000) Canada Pacific Canadian Journal of Zoology 75 12 2112 2119 |
institution |
Open Polar |
collection |
Canadian Science Publishing |
op_collection_id |
crcansciencepubl |
language |
English |
description |
Myxosporeans in the genus Kudoa infect the flesh of many marine fishes and often cause unsightly lesions and softening of the flesh texture. We are particularly interested in K. thyrsites because it is associated with soft flesh in the Atlantic salmon (Salmo solar), an important commercial species in Canada. Sequences of the small-subunit (SSU) rDNA (about 1600 base pairs) were obtained from K. miniauriculata, K. amanuensis, and K. poniformis. We aligned these sequences with one obtained from coho salmon (Oncorhynchus kisutch) and designed "Kudoa general" primers (KUD1f and KUD2r). These primers, in combination with other general primers, were then used to obtain the SSU rDNA sequence of K. thyrsites from two host species, Atlantic salmon and tubesnout (Aulorhynchus flavidus), from British Columbia, Canada. Sequence comparisons of these isolates indicated that Kudoa species cluster by geographic location rather than by morphology of spores. The three species from the eastern Pacific were approximately 97% identical, whereas K. amamiensis (from Japan) was about 91% identical with these species. Sequence comparisons of K. thyrsites from Atlantic salmon and tubesnout revealed a difference of only 0.07% between these isolates. Comparison of SSU rDNA sequences from the four Kudoa species and Henneguya salminicolo analyzed in this study with those from other available myxosporean genera (Myxidium and Myxoholus) showed that taxonomic divisions at the order and suborder levels were consistent with classical views of the taxonomy of the Myxosporea. Using specific regions of the SSU rDNA, we also developed a sensitive and specific polymerase chain reaction test for detection of K. thyrsites. |
format |
Article in Journal/Newspaper |
author |
Hervio, D. M. L. Khattra, J. Devlin, R. H. Kent, M. L. Sakanari, J. Yokoyama, H. |
spellingShingle |
Hervio, D. M. L. Khattra, J. Devlin, R. H. Kent, M. L. Sakanari, J. Yokoyama, H. Taxonomy of Kudoa species (Myxosporea), using a small-subunit ribosomal DNA sequence |
author_facet |
Hervio, D. M. L. Khattra, J. Devlin, R. H. Kent, M. L. Sakanari, J. Yokoyama, H. |
author_sort |
Hervio, D. M. L. |
title |
Taxonomy of Kudoa species (Myxosporea), using a small-subunit ribosomal DNA sequence |
title_short |
Taxonomy of Kudoa species (Myxosporea), using a small-subunit ribosomal DNA sequence |
title_full |
Taxonomy of Kudoa species (Myxosporea), using a small-subunit ribosomal DNA sequence |
title_fullStr |
Taxonomy of Kudoa species (Myxosporea), using a small-subunit ribosomal DNA sequence |
title_full_unstemmed |
Taxonomy of Kudoa species (Myxosporea), using a small-subunit ribosomal DNA sequence |
title_sort |
taxonomy of kudoa species (myxosporea), using a small-subunit ribosomal dna sequence |
publisher |
Canadian Science Publishing |
publishDate |
1997 |
url |
http://dx.doi.org/10.1139/z97-846 http://www.nrcresearchpress.com/doi/pdf/10.1139/z97-846 |
long_lat |
ENVELOPE(-125.003,-125.003,54.000,54.000) |
geographic |
British Columbia Canada Pacific |
geographic_facet |
British Columbia Canada Pacific |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Canadian Journal of Zoology volume 75, issue 12, page 2112-2119 ISSN 0008-4301 1480-3283 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/z97-846 |
container_title |
Canadian Journal of Zoology |
container_volume |
75 |
container_issue |
12 |
container_start_page |
2112 |
op_container_end_page |
2119 |
_version_ |
1802642305353515008 |