Determination of gender in cetaceans by the polymerase chain reaction
We determined the gender of a variety of cetacean species, including both ondotocetes and mysticetes, using the polymerase chain reaction for amplification of the sex chromosome specific regions ZFY/ZFX and SRY. This quick and simple method requires extremely small amounts of tissue, and therefore a...
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Canadian Science Publishing
1992
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Online Access: | http://dx.doi.org/10.1139/z92-292 http://www.nrcresearchpress.com/doi/pdf/10.1139/z92-292 |
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crcansciencepubl:10.1139/z92-292 2024-06-23T07:51:33+00:00 Determination of gender in cetaceans by the polymerase chain reaction Palsbøll, Per J. Vader, Anna Bakke, Ingrid El-Gewely, M. Raafat 1992 http://dx.doi.org/10.1139/z92-292 http://www.nrcresearchpress.com/doi/pdf/10.1139/z92-292 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Zoology volume 70, issue 11, page 2166-2170 ISSN 0008-4301 1480-3283 journal-article 1992 crcansciencepubl https://doi.org/10.1139/z92-292 2024-05-30T08:13:47Z We determined the gender of a variety of cetacean species, including both ondotocetes and mysticetes, using the polymerase chain reaction for amplification of the sex chromosome specific regions ZFY/ZFX and SRY. This quick and simple method requires extremely small amounts of tissue, and therefore allows gender to be determined from skin biopsies taken from free-ranging specimens. In the fin whale, Balaenoptera physalus, no gender-specific bands were observed when the ZFY/ZFX system was used, but when the SRY system was used, sex was accurately determined. Previous studies in other mammals have also shown the SRY system to be more reliable in sex determination. We therefore recommend amplification of the SRY region alone or in parallel with the ZFY/ZFX regions, as described here, as a test for gender in cetaceans and other mammals. Article in Journal/Newspaper Balaenoptera physalus Fin whale Canadian Science Publishing Canadian Journal of Zoology 70 11 2166 2170 |
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Canadian Science Publishing |
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crcansciencepubl |
language |
English |
description |
We determined the gender of a variety of cetacean species, including both ondotocetes and mysticetes, using the polymerase chain reaction for amplification of the sex chromosome specific regions ZFY/ZFX and SRY. This quick and simple method requires extremely small amounts of tissue, and therefore allows gender to be determined from skin biopsies taken from free-ranging specimens. In the fin whale, Balaenoptera physalus, no gender-specific bands were observed when the ZFY/ZFX system was used, but when the SRY system was used, sex was accurately determined. Previous studies in other mammals have also shown the SRY system to be more reliable in sex determination. We therefore recommend amplification of the SRY region alone or in parallel with the ZFY/ZFX regions, as described here, as a test for gender in cetaceans and other mammals. |
format |
Article in Journal/Newspaper |
author |
Palsbøll, Per J. Vader, Anna Bakke, Ingrid El-Gewely, M. Raafat |
spellingShingle |
Palsbøll, Per J. Vader, Anna Bakke, Ingrid El-Gewely, M. Raafat Determination of gender in cetaceans by the polymerase chain reaction |
author_facet |
Palsbøll, Per J. Vader, Anna Bakke, Ingrid El-Gewely, M. Raafat |
author_sort |
Palsbøll, Per J. |
title |
Determination of gender in cetaceans by the polymerase chain reaction |
title_short |
Determination of gender in cetaceans by the polymerase chain reaction |
title_full |
Determination of gender in cetaceans by the polymerase chain reaction |
title_fullStr |
Determination of gender in cetaceans by the polymerase chain reaction |
title_full_unstemmed |
Determination of gender in cetaceans by the polymerase chain reaction |
title_sort |
determination of gender in cetaceans by the polymerase chain reaction |
publisher |
Canadian Science Publishing |
publishDate |
1992 |
url |
http://dx.doi.org/10.1139/z92-292 http://www.nrcresearchpress.com/doi/pdf/10.1139/z92-292 |
genre |
Balaenoptera physalus Fin whale |
genre_facet |
Balaenoptera physalus Fin whale |
op_source |
Canadian Journal of Zoology volume 70, issue 11, page 2166-2170 ISSN 0008-4301 1480-3283 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/z92-292 |
container_title |
Canadian Journal of Zoology |
container_volume |
70 |
container_issue |
11 |
container_start_page |
2166 |
op_container_end_page |
2170 |
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1802642663428587520 |