The effects of temperature acclimation on protein synthesis rates and nucleic acid content of juvenile cod ( Gadus morhua L.)
Juvenile cod were acclimated to cold (5 °C) and warm (15 °C) water temperatures and fed sandeel at a similar ration size (3% body weight∙day −1 ) for at least 40 days. After this acclimation period, there were no significant differences in either weight-specific growth rate or weight-specific tissue...
Published in: | Canadian Journal of Zoology |
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Canadian Science Publishing
1992
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crcansciencepubl:10.1139/z92-282 2024-09-30T14:35:17+00:00 The effects of temperature acclimation on protein synthesis rates and nucleic acid content of juvenile cod ( Gadus morhua L.) Foster, A. R. Houlihan, D. F. Hall, S. J. Burren, L. J. 1992 http://dx.doi.org/10.1139/z92-282 http://www.nrcresearchpress.com/doi/pdf/10.1139/z92-282 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Zoology volume 70, issue 11, page 2095-2102 ISSN 0008-4301 1480-3283 journal-article 1992 crcansciencepubl https://doi.org/10.1139/z92-282 2024-09-05T04:11:16Z Juvenile cod were acclimated to cold (5 °C) and warm (15 °C) water temperatures and fed sandeel at a similar ration size (3% body weight∙day −1 ) for at least 40 days. After this acclimation period, there were no significant differences in either weight-specific growth rate or weight-specific tissue protein synthesis rates (ventricle, gill, stomach, and intestine) between the cold- and warm-acclimated fish. However, every cold-acclimated tissue examined had a significantly higher RNA concentration (μg RNA∙g tissue −1 ) than the respective warm-acclimated tissue. Cold-acclimated ventricle and intestine had significantly reduced RNA activities (i.e., translational efficiency, g protein synthesized∙g RNA −1 ∙day −1 ) compared with the warm-acclimated tissues. In contrast, the mean RNA activities of cold-acclimated stomach and gill were not significantly different from those of the same tissues in the warm-acclimated fish. These alterations in RNA activity and RNA concentration with temperature acclimation probably represent a thermal compensatory mechanism for protein synthesis and growth in cod at 5 °C. Positive linear relationships were observed between tissue protein synthesis rates and tissue RNA concentrations (μg RNA∙g tissue −1 ). RNA/protein ratios (μg RNA∙mg protein −1 ) gave a positive (but statistically insignificant) trend with protein synthesis rates. In contrast, a negative trend (statistically insignificant) was observed between tissue protein synthesis rates and tissue RNA/DNA ratios (μg RNA∙μg DNA −1 ). The use of RNA measurements as biochemical correlates of growth rate in juvenile cod is discussed. Article in Journal/Newspaper Gadus morhua Canadian Science Publishing Canadian Journal of Zoology 70 11 2095 2102 |
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Open Polar |
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Canadian Science Publishing |
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English |
description |
Juvenile cod were acclimated to cold (5 °C) and warm (15 °C) water temperatures and fed sandeel at a similar ration size (3% body weight∙day −1 ) for at least 40 days. After this acclimation period, there were no significant differences in either weight-specific growth rate or weight-specific tissue protein synthesis rates (ventricle, gill, stomach, and intestine) between the cold- and warm-acclimated fish. However, every cold-acclimated tissue examined had a significantly higher RNA concentration (μg RNA∙g tissue −1 ) than the respective warm-acclimated tissue. Cold-acclimated ventricle and intestine had significantly reduced RNA activities (i.e., translational efficiency, g protein synthesized∙g RNA −1 ∙day −1 ) compared with the warm-acclimated tissues. In contrast, the mean RNA activities of cold-acclimated stomach and gill were not significantly different from those of the same tissues in the warm-acclimated fish. These alterations in RNA activity and RNA concentration with temperature acclimation probably represent a thermal compensatory mechanism for protein synthesis and growth in cod at 5 °C. Positive linear relationships were observed between tissue protein synthesis rates and tissue RNA concentrations (μg RNA∙g tissue −1 ). RNA/protein ratios (μg RNA∙mg protein −1 ) gave a positive (but statistically insignificant) trend with protein synthesis rates. In contrast, a negative trend (statistically insignificant) was observed between tissue protein synthesis rates and tissue RNA/DNA ratios (μg RNA∙μg DNA −1 ). The use of RNA measurements as biochemical correlates of growth rate in juvenile cod is discussed. |
format |
Article in Journal/Newspaper |
author |
Foster, A. R. Houlihan, D. F. Hall, S. J. Burren, L. J. |
spellingShingle |
Foster, A. R. Houlihan, D. F. Hall, S. J. Burren, L. J. The effects of temperature acclimation on protein synthesis rates and nucleic acid content of juvenile cod ( Gadus morhua L.) |
author_facet |
Foster, A. R. Houlihan, D. F. Hall, S. J. Burren, L. J. |
author_sort |
Foster, A. R. |
title |
The effects of temperature acclimation on protein synthesis rates and nucleic acid content of juvenile cod ( Gadus morhua L.) |
title_short |
The effects of temperature acclimation on protein synthesis rates and nucleic acid content of juvenile cod ( Gadus morhua L.) |
title_full |
The effects of temperature acclimation on protein synthesis rates and nucleic acid content of juvenile cod ( Gadus morhua L.) |
title_fullStr |
The effects of temperature acclimation on protein synthesis rates and nucleic acid content of juvenile cod ( Gadus morhua L.) |
title_full_unstemmed |
The effects of temperature acclimation on protein synthesis rates and nucleic acid content of juvenile cod ( Gadus morhua L.) |
title_sort |
effects of temperature acclimation on protein synthesis rates and nucleic acid content of juvenile cod ( gadus morhua l.) |
publisher |
Canadian Science Publishing |
publishDate |
1992 |
url |
http://dx.doi.org/10.1139/z92-282 http://www.nrcresearchpress.com/doi/pdf/10.1139/z92-282 |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_source |
Canadian Journal of Zoology volume 70, issue 11, page 2095-2102 ISSN 0008-4301 1480-3283 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/z92-282 |
container_title |
Canadian Journal of Zoology |
container_volume |
70 |
container_issue |
11 |
container_start_page |
2095 |
op_container_end_page |
2102 |
_version_ |
1811638606396129280 |