Yolk proteins in salmon ( Salmo salar) oocytes, eyed eggs, and alevins differing in viability
The developmental stages of oocytes, eyed eggs, and alevins from salmon (Salmo salar) were compared for their yolk protein composition. In oocytes, SDS–polyacrylamide gel electrophoresis showed high amounts of a protein with the molecular weight (M r ) of 94 000. In eyed eggs, the 94 000 protein dec...
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Canadian Science Publishing
1990
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crcansciencepubl:10.1139/z90-130 2024-09-15T18:33:02+00:00 Yolk proteins in salmon ( Salmo salar) oocytes, eyed eggs, and alevins differing in viability Olin, Thomas Decken, Alexandra von der 1990 http://dx.doi.org/10.1139/z90-130 http://www.nrcresearchpress.com/doi/pdf/10.1139/z90-130 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Zoology volume 68, issue 5, page 895-900 ISSN 0008-4301 1480-3283 journal-article 1990 crcansciencepubl https://doi.org/10.1139/z90-130 2024-06-27T04:11:01Z The developmental stages of oocytes, eyed eggs, and alevins from salmon (Salmo salar) were compared for their yolk protein composition. In oocytes, SDS–polyacrylamide gel electrophoresis showed high amounts of a protein with the molecular weight (M r ) of 94 000. In eyed eggs, the 94 000 protein decreased and was undetectable in the alevins. Furthermore, in eyed eggs the proteins of 67 000, 30 000, and 27 000 increased, while in the alevins the concentration of the 67 000 protein decreased and that of the 39 000 increased. Vitellogenin-specific antigen sites analyzed by immunoblotting were most pronounced with the proteins of 94 000, 67 000, 39 000, 30 000, 23 000, and 19 000. Separation of the yolk proteins by HPLC gave four peaks at 280 nm for all three developmental stages. Each peak consisted of several proteins as analyzed by SDS–polyacrylamide gel electrophoresis. The 7-day-old alevins sampled from groups of different parental origin showed differences in the amount of the 67 000 and 23 000 proteins. Expectancy of survival within the group in connection with a slow disappearance of the 67 000 and 23 000 proteins was statistically significant. A fast disappearance may be used as an indication of, but not as the reason for, a high mortality within one group of alevins. Article in Journal/Newspaper Salmo salar Canadian Science Publishing Canadian Journal of Zoology 68 5 895 900 |
institution |
Open Polar |
collection |
Canadian Science Publishing |
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crcansciencepubl |
language |
English |
description |
The developmental stages of oocytes, eyed eggs, and alevins from salmon (Salmo salar) were compared for their yolk protein composition. In oocytes, SDS–polyacrylamide gel electrophoresis showed high amounts of a protein with the molecular weight (M r ) of 94 000. In eyed eggs, the 94 000 protein decreased and was undetectable in the alevins. Furthermore, in eyed eggs the proteins of 67 000, 30 000, and 27 000 increased, while in the alevins the concentration of the 67 000 protein decreased and that of the 39 000 increased. Vitellogenin-specific antigen sites analyzed by immunoblotting were most pronounced with the proteins of 94 000, 67 000, 39 000, 30 000, 23 000, and 19 000. Separation of the yolk proteins by HPLC gave four peaks at 280 nm for all three developmental stages. Each peak consisted of several proteins as analyzed by SDS–polyacrylamide gel electrophoresis. The 7-day-old alevins sampled from groups of different parental origin showed differences in the amount of the 67 000 and 23 000 proteins. Expectancy of survival within the group in connection with a slow disappearance of the 67 000 and 23 000 proteins was statistically significant. A fast disappearance may be used as an indication of, but not as the reason for, a high mortality within one group of alevins. |
format |
Article in Journal/Newspaper |
author |
Olin, Thomas Decken, Alexandra von der |
spellingShingle |
Olin, Thomas Decken, Alexandra von der Yolk proteins in salmon ( Salmo salar) oocytes, eyed eggs, and alevins differing in viability |
author_facet |
Olin, Thomas Decken, Alexandra von der |
author_sort |
Olin, Thomas |
title |
Yolk proteins in salmon ( Salmo salar) oocytes, eyed eggs, and alevins differing in viability |
title_short |
Yolk proteins in salmon ( Salmo salar) oocytes, eyed eggs, and alevins differing in viability |
title_full |
Yolk proteins in salmon ( Salmo salar) oocytes, eyed eggs, and alevins differing in viability |
title_fullStr |
Yolk proteins in salmon ( Salmo salar) oocytes, eyed eggs, and alevins differing in viability |
title_full_unstemmed |
Yolk proteins in salmon ( Salmo salar) oocytes, eyed eggs, and alevins differing in viability |
title_sort |
yolk proteins in salmon ( salmo salar) oocytes, eyed eggs, and alevins differing in viability |
publisher |
Canadian Science Publishing |
publishDate |
1990 |
url |
http://dx.doi.org/10.1139/z90-130 http://www.nrcresearchpress.com/doi/pdf/10.1139/z90-130 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
Canadian Journal of Zoology volume 68, issue 5, page 895-900 ISSN 0008-4301 1480-3283 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/z90-130 |
container_title |
Canadian Journal of Zoology |
container_volume |
68 |
container_issue |
5 |
container_start_page |
895 |
op_container_end_page |
900 |
_version_ |
1810474790593495040 |